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anti-PHLPP1 antibody :: PHLPP1 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS9130452
Western blot
Unit / Price
0.05 mL  /  $230 +1 FREE 8GB USB
0.1 mL  /  $305 +1 FREE 8GB USB
0.2 mL  /  $460 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

PHLPP1, Polyclonal Antibody

★Popular Item★
 Also Known As   

PHLPP1 Polyclonal Antibody

 Product Synonym Names    PHLPP;PLEKHE1;PPM3A;SCOP
 Product Gene Name   

anti-PHLPP1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    609396
 3D Structure    ModBase 3D Structure for O60346
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 Clonality    Polyclonal
 Isotype    IgG
 Species Reactivity    Human,Mouse,Rat
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 Purity/Purification    Affinity purification
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 Source    Rabbit
 Immunogen    Recombinant protein of human PHLPP1
 Category    Primary antibody
 Observed MW    185kDa
 Calculated MW    133kDa/184kDa
 Buffer    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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 Preparation and Storage    Store at -20 degree C. Avoid freeze / thaw cycles.
 Other Notes    Small volumes of anti-PHLPP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-PHLPP1 antibody

   This gene encodes a member of the serine/threonine phosphatase family. The encoded protein promotes apoptosis by dephosphorylating and inactivating the serine/threonine kinase Akt, and functions as a tumor suppressor in multiple types of cancer. Increased expression of this gene may also play a role in obesity and type 2 diabetes by interfering with Akt-mediated insulin signaling.
 Product Categories/Family for anti-PHLPP1 antibody    Polyclonal
 Applications Tested/Suitable for anti-PHLPP1 antibody   

Western Blot (WB)

 Application Notes for anti-PHLPP1 antibody    WB: 1:500 - 1:2000
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 Western blot of anti-PHLPP1 antibody    Western blot analysis of extracts of various cell lines, using PHLPP1 antibody (MBS9130452) at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (Please inquire). Exposure time: 10s.
anti-PHLPP1 antibody Western blot image
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NCBI/Uniprot data below describe general gene information for PHLPP1. It may not necessarily be applicable to this product.
 NCBI GI #    291219891
 NCBI GeneID    23239
 NCBI Accession #    NP_919431.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_194449.3 [Other Products]
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 UniProt Primary Accession #    O60346 [Other Products]
 UniProt Secondary Accession #    Q641Q7; Q6P4C4; Q6PJI6; Q86TN6; Q96FK2; Q9NUY1; A1A4F5 [Other Products]
 UniProt Related Accession #    O60346 [Other Products]
 Molecular Weight    Calculated MW: 185kDa
Molecular Weight: 1717
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 NCBI Official Full Name    PH domain leucine-rich repeat-containing protein phosphatase 1
 NCBI Official Synonym Full Names    PH domain and leucine rich repeat protein phosphatase 1
 NCBI Official Symbol    PHLPP1 [Similar Products]
 NCBI Official Synonym Symbols   
SCOP; PHLPP; PPM3A; PLEKHE1
[Similar Products]
 NCBI Protein Information    PH domain leucine-rich repeat-containing protein phosphatase 1
 UniProt Protein Name    PH domain leucine-rich repeat-containing protein phosphatase 1
 UniProt Synonym Protein Names   
Pleckstrin homology domain-containing family E member 1; PH domain-containing family E member 1; Suprachiasmatic nucleus circadian oscillatory protein; hSCOP
 Protein Family    PH domain leucine-rich repeat protein phosphatase
 UniProt Gene Name    PHLPP1 [Similar Products]
 UniProt Synonym Gene Names    KIAA0606; PHLPP; PLEKHE1; SCOP; PH domain-containing family E member 1; hSCOP [Similar Products]
 UniProt Entry Name    PHLP1_HUMAN
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 NCBI Summary for PHLPP1    This gene encodes a member of the serine/threonine phosphatase family. The encoded protein promotes apoptosis by dephosphorylating and inactivating the serine/threonine kinase Akt, and functions as a tumor suppressor in multiple types of cancer. Increased expression of this gene may also play a role in obesity and type 2 diabetes by interfering with Akt-mediated insulin signaling. [provided by RefSeq, Dec 2011]
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 UniProt Comments for PHLPP1    PHLPP: a pleckstrin homology domain leucine-rich repeat protein phosphoserine and ¿threonine phosphatase. A tumor suppressor that controls the onset and progression of various cancers because it controls the balance between cell survival and apoptosis. Catalyzes the dephosphorylation of the hydrophobic motif on the AGC kinases Akt, PKC, and S6 kinase, and the inhibitory site on the kinase Mst1. The dephosphorylation of PKCA and PKCB is destabilizing, leading to its degradation. The dephosphorylation of p70S6K at T390 reduces its activity, thus reducing the efficiency of protein translation. Dephosphorylation of Mst1 by PHLPP of an autoinhibitory site (T387) activates Mst1, resulting in the activation of the pro-apoptotic MAPK pathway. Ubiquitously expressed in non-cancerous tissues. Insensitive to inhibition by okadaic acid. PHLPP loss occurs at high frequency in colorectal cancer, and leads to the development of prostate tumors in mice. PHLPP degradation by the 26 S proteasome is controlled by a beta-TrCP-containing Skp¿Cullin 1¿F-box protein (SCF) complex E3 ubiquitin ligase in a phosphorylation-dependent manner. This degradation requires phosphorylation of PHLPP1 by CK I and GSK-3beta. Activation of the PI3K/Akt pathway suppresses the degradation of PHLPP1 by inhibiting GSK-3beta activity. PHLPP2 activity is highly sensitive to a common polymorphism (L-1016-S) that reduces its rate of dephosphorylation of the hydrophobic motif of Akt and PKC, rendering it ineffective at inducing apoptosis. This variant is expressed by 30% of the human population. Interacts with the nucleotide free form of KRas via its LRR repeats. Interacts with AKT2, AKT3 and PKCB isoform beta-II.

Protein type: EC 3.1.3.16; Tumor suppressor; Protein phosphatase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 18q21.33

Cellular Component: cytosol; nucleus; plasma membrane

Molecular Function: metal ion binding; phosphoprotein phosphatase activity; protein binding

Biological Process: apoptosis; entrainment of circadian clock; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; innate immune response; negative regulation of protein kinase B signaling cascade; nerve growth factor receptor signaling pathway; phosphoinositide-mediated signaling; protein amino acid dephosphorylation; regulation of apoptosis; regulation of JNK cascade; regulation of MAPKKK cascade; regulation of T cell anergy
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 Research Articles on PHLPP1    1. Aberrant expression of PHLPP1 and PHLPP2 correlates with poor prognosis in patients with hypopharyngeal squamous cell carcinoma
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-PHLPP1 antibodyDiseases associated with anti-PHLPP1 antibody
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 DAP12 Interactions Pathway antibodies  DAP12 Interactions Pathway Diagram
 DAP12 Signaling Pathway antibodies  DAP12 Signaling Pathway Diagram
 Downstream Signal Transduction Pathway antibodies  Downstream Signal Transduction Pathway Diagram
 Downstream Signaling Events Of B Cell Receptor (BCR) Pathway antibodies  Downstream Signaling Events Of B Cell Receptor (BCR) Pathway Diagram
 Downstream Signaling Of Activated FGFR1 Pathway antibodies  Downstream Signaling Of Activated FGFR1 Pathway Diagram
 Downstream Signaling Of Activated FGFR2 Pathway antibodies  Downstream Signaling Of Activated FGFR2 Pathway Diagram
 Downstream Signaling Of Activated FGFR3 Pathway antibodies  Downstream Signaling Of Activated FGFR3 Pathway Diagram
 Downstream Signaling Of Activated FGFR4 Pathway antibodies  Downstream Signaling Of Activated FGFR4 Pathway Diagram
 Fc Epsilon Receptor (FCERI) Signaling Pathway antibodies  Fc Epsilon Receptor (FCERI) Signaling Pathway Diagram
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >13 publications with PHLPP1 and Carcinoma
 Neoplasm Metastasis Antibodies  >6 publications with PHLPP1 and Neoplasm Metastasis
 Nervous System Diseases Antibodies  >6 publications with PHLPP1 and Nervous System Diseases
 Breast Neoplasms Antibodies  >5 publications with PHLPP1 and Breast Neoplasms
 Cardiovascular Diseases Antibodies  >4 publications with PHLPP1 and Cardiovascular Diseases
 Immune System Diseases Antibodies  >4 publications with PHLPP1 and Immune System Diseases
 Liver Diseases Antibodies  >4 publications with PHLPP1 and Liver Diseases
 Neoplasms, Experimental Antibodies  >4 publications with PHLPP1 and Neoplasms, Experimental
 Cell Transformation, Neoplastic Antibodies  >4 publications with PHLPP1 and Cell Transformation, Neoplastic
 Hypertrophy Antibodies  >3 publications with PHLPP1 and Hypertrophy
Organs/Tissues associated with anti-PHLPP1 antibody
 Organ/Tissue Name  Pubmed Publications
 Prostate Antibodies  >15 publications with PHLPP1 and Prostate
 Brain Antibodies  >14 publications with PHLPP1 and Brain
 Muscle Antibodies  >5 publications with PHLPP1 and Muscle
 Liver Antibodies  >5 publications with PHLPP1 and Liver
 Lung Antibodies  >4 publications with PHLPP1 and Lung
 Blood Antibodies  >4 publications with PHLPP1 and Blood
 Eye Antibodies  >4 publications with PHLPP1 and Eye
 Nerve Antibodies  >3 publications with PHLPP1 and Nerve
 Lymph Node Antibodies  >3 publications with PHLPP1 and Lymph Node
 Lymph Antibodies  >3 publications with PHLPP1 and Lymph
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