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anti-PIAS2 antibody :: Rabbit PIAS2 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS150305
Unit / Price
0.02 mg  /  $140 +1 FREE 8GB USB
0.1 mg  /  $355 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

PIAS2, Polyclonal Antibody

 Also Known As   

PIAS2 Antibody

 Product Synonym Names    PIAS2; DIP; MIZ; MIZ1; SIZ2; ARIP3; PIASX; ZMIZ4; PIASX-BETA; PIASX-ALPHA; E3 SUMO-protein ligase PIAS2; Androgen receptor-interacting protein 3; protein inhibitor of activated STAT, 2
 Product Gene Name   

anti-PIAS2 antibody

[Similar Products]
 Antibody/Peptide Pairs    PIAS2 peptide (MBS153232) is used for blocking the activity of PIAS2 antibody (MBS150305)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    603567
 3D Structure    ModBase 3D Structure for O75928
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human, Mouse, Rat
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 Purity/Purification    PIAS2 Antibody is affinity chromatography purified via peptide column.
 Form/Format    Liquid
 Concentration    1 mg/mL (lot specific)
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 Conjugate    Unconjugated
 Immunogen    PIAS2 antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of human PIAS2.
 Buffer    PIAS2 Antibody is supplied in PBS containing 0.02% sodium azide.
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 Preparation and Storage    PIAS2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
 Other Notes    Small volumes of anti-PIAS2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-PIAS2 antibody

   PIAS2 Antibody: The PIAS (protein inhibitor of activated STAT) proteins play a crucial role as transcriptional coregulators in various cellular pathways, including the STAT, p53 and the steroid hormone signaling pathway. The PIAS protein family includes at least five evolutionarily conserved genes, including PIAS2. The major function of the PIAS proteins is the control of gene transcription and can also act as small ubiquitin-like-modifier (SUMO) E3 ligases. PIAS2 binds specifically to STAT4 following IL-12 stimulation and inhibits STAT4-mediated gene activation in human T cells. PIAS2 is a potent transcriptional activator of Bcl-2, but together with Bcl-6 can suppress the expression of Bcl-2.
 Applications Tested/Suitable for anti-PIAS2 antibody   

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

 Application Notes for anti-PIAS2 antibody    PIAS2 antibody can be used for detection of PIAS2 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
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 Western Blot (WB) of anti-PIAS2 antibody    Western blot analysis of PIAS2 in rat brain tissue lysate with PIAS2 antibody at (A) 1 and (B) 2 μg/mL.
anti-PIAS2 antibody Western Blot (WB) (WB) image
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 Immunofluorescence (IF) of anti-PIAS2 antibody    Immunofluorescence of PIAS2 in rat brain tissue with PIAS2 antibody at 20 μg/mL.
anti-PIAS2 antibody Immunofluorescence (IF) image
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 Immunohistochemistry (IHC) of anti-PIAS2 antibody    Immunohistochemistry of PIAS2 in rat brain tissue with PIAS2 antibody at 5 μg/mL.
anti-PIAS2 antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for PIAS2. It may not necessarily be applicable to this product.
 NCBI GI #    119621890
 NCBI GeneID    9063
 NCBI Accession #    EAX01485 [Other Products]
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 UniProt Primary Accession #    O75928 [Other Products]
 UniProt Secondary Accession #    O75927; Q96BT5; Q96KE3 [Other Products]
 UniProt Related Accession #    O75928 [Other Products]
 Molecular Weight    45,057 Da
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 NCBI Official Full Name    protein inhibitor of activated STAT, 2, isoform CRA_b
 NCBI Official Synonym Full Names    protein inhibitor of activated STAT, 2
 NCBI Official Symbol    PIAS2 [Similar Products]
 NCBI Official Synonym Symbols   
DIP; MIZ; MIZ1; SIZ2; ARIP3; PIASX; ZMIZ4; PIASX-BETA; PIASX-ALPHA
[Similar Products]
 NCBI Protein Information    E3 SUMO-protein ligase PIAS2; DAB2-interacting protein; zinc finger, MIZ-type containing 4; msx-interacting zinc finger protein; protein inhibitor of activated STAT X; androgen receptor-interacting protein 3
 UniProt Protein Name    E3 SUMO-protein ligase PIAS2
 UniProt Synonym Protein Names   
Androgen receptor-interacting protein 3; ARIP3; DAB2-interacting protein; DIP; Msx-interacting zinc finger protein; Miz1; PIAS-NY protein; Protein inhibitor of activated STAT x; Protein inhibitor of activated STAT2
 Protein Family    E3 SUMO-protein ligase
 UniProt Gene Name    PIAS2 [Similar Products]
 UniProt Synonym Gene Names    PIASX; ARIP3; DIP; Miz1 [Similar Products]
 UniProt Entry Name    PIAS2_HUMAN
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 NCBI Summary for PIAS2    This gene encodes a member of the protein inhibitor of activated STAT (PIAS) family. PIAS proteins function as SUMO E3 ligases and play important roles in many cellular processes by mediating the sumoylation of target proteins. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Isoforms of the encoded protein enhance the sumoylation of specific target proteins including the p53 tumor suppressor protein, c-Jun, and the androgen receptor. A pseudogene of this gene is located on the short arm of chromosome 4. The symbol MIZ1 has also been associated with ZBTB17 which is a different gene located on chromosome 1. [provided by RefSeq, Aug 2011]
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 UniProt Comments for PIAS2    PIAS2: Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIAS2-beta, but not isoform PIAS2-alpha, promotes MDM2 sumoylation. Isoform PIAS2-alpha promotes PARK7 sumoylation. Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2- alpha. Belongs to the PIAS family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.-; SUMO conjugating system; Transcription, coactivator/corepressor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 18q21.1

Cellular Component: PML body; nuclear speck; nucleus

Molecular Function: protein binding; DNA binding; androgen receptor binding; zinc ion binding; ubiquitin protein ligase binding; transcription coactivator activity; transcription factor binding; ligase activity

Biological Process: protein sumoylation; transcription, DNA-dependent; regulation of osteoblast differentiation; positive regulation of transcription, DNA-dependent; negative regulation of transcription factor activity; androgen receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter
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 Research Articles on PIAS2    1. PIASxalpha is a novel SUMO E3 ligase for PTEN, and it positively regulates PTEN protein level in tumor suppression.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-PIAS2 antibodyPathways associated with anti-PIAS2 antibody
 Reference Product  PubMed Publications
 SUMO1 antibody  >22 publications with PIAS2 and SUMO1
 STAT1 antibody  >4 publications with PIAS2 and STAT1
 MYC antibody  >3 publications with PIAS2 and MYC
 SMAD4 antibody  >1 publications with PIAS2 and SMAD4
 STAT4 antibody  >1 publications with PIAS2 and STAT4
 Products by Pathway  Pathway Diagram
 IL12 Signaling Mediated By STAT4 Pathway antibodies  IL12 Signaling Mediated By STAT4 Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Pathways In Cancer antibodies  Pathways In Cancer Diagram
 Small Cell Lung Cancer Pathway antibodies  Small Cell Lung Cancer Pathway Diagram
 Small Cell Lung Cancer Pathway antibodies  Small Cell Lung Cancer Pathway Diagram
 Sumoylation By RanBP2 Regulates Transcriptional Repression Pathway antibodies  Sumoylation By RanBP2 Regulates Transcriptional Repression Pathway Diagram
 TGF-beta Receptor Signaling Pathway antibodies  TGF-beta Receptor Signaling Pathway Diagram
 Ubiquitin Mediated Proteolysis Pathway antibodies  Ubiquitin Mediated Proteolysis Pathway Diagram
 Ubiquitin Mediated Proteolysis Pathway antibodies  Ubiquitin Mediated Proteolysis Pathway Diagram
Diseases associated with anti-PIAS2 antibodyOrgans/Tissues associated with anti-PIAS2 antibody
 Disease Name  Pubmed Publications
 Immune System Diseases Antibodies  >3 publications with PIAS2 and Immune System Diseases
 Brain Diseases Antibodies  >1 publications with PIAS2 and Brain Diseases
 Chromosome Aberrations Antibodies  >1 publications with PIAS2 and Chromosome Aberrations
 Fibrosis Antibodies  >1 publications with PIAS2 and Fibrosis
 Nerve Degeneration Antibodies  >1 publications with PIAS2 and Nerve Degeneration
 Inflammation Antibodies  >1 publications with PIAS2 and Inflammation
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >3 publications with PIAS2 and Bone
 Brain Antibodies  >3 publications with PIAS2 and Brain
 Muscle Antibodies  >2 publications with PIAS2 and Muscle
 Liver Antibodies  >1 publications with PIAS2 and Liver
 Pancreas Antibodies  >1 publications with PIAS2 and Pancreas
 Testis Antibodies  >1 publications with PIAS2 and Testis
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