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| Product Name
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PIK3CA/P110alpha, Polyclonal Antibody ★Popular Item★
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| Also Known As
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Goat anti-PIK3CA/P110alpha Antibody
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| Product Synonym Names
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PIK3CA; p110-alpha; phosphoinositide-3-kinase, catalytic, alpha polypeptide; MGC142161; MGC142163; PI3K; PI3-kinase p110 subunit alpha; PtdIns-3-kinase p110; phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha; phosphatidylinositol 3-kinase, catalyti; PIK3CA antibody; p110-alpha antibody; phosphoinositide-3-kinase; catalytic; alpha polypeptide antibody; MGC142161 antibody; MGC142163 antibody; PI3K antibody; PI3-kinase p110 subunit alpha antibody; PtdIns-3-kinase p110 antibody; phosphatidylinositol 3-kinase; catalytic; 110-KD; alpha antibody; phosphatidylinositol 3-kinase; catalytic; alpha polypeptide antibody; phosphatidylinositol-4; 5-bisphosphate 3-kinase catalytic subunit; alpha isoform antibody; PIK3CA / P110alpha
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| Product Gene Name
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[Similar Products]
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| Product Synonym Gene Name
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P110alpha
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[Similar Products]
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| Antibody/Peptide Pairs
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PIK3CA / P110alpha peptide (MBS426574) is used for blocking the activity of PIK3CA/P110alpha antibody (MBS421100)
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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 TOP
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| Immunogen Sequence
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HQWLKDKNKG EIYD
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| OMIM
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114480
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| Clonality
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Polyclonal
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| Host
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Goat
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| Species Reactivity
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Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
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| Purity/Purification
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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| Form/Format
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Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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| Concentration
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100ug specific antibody in 200ul (lot specific)
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| Immunogen
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Peptide with sequence C-HQWLKDKNKGEIYD, from the internal region of the protein sequence according to NP_006209.2.
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| Epitope
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Internal region
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| Preparation and Storage
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Aliquot and store at -20 degree C. Minimize freezing and thawing.
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| ISO Certification
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Manufactured in an ISO 9001:2015 Certified Laboratory.
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| Other Notes
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Small volumes of anti-PIK3CA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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| Applications Tested/Suitable for anti-PIK3CA antibody
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Peptide ELISA (EIA), Immunohistochemistry (IHC)
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| Application Notes for anti-PIK3CA antibody
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Peptide ELISA: Antibody detection limit dilution 1: 128000.
Immunohistochemistry: In paraffin embedded Human Brain (Cerebral Cortex) shows cytoplasm stained in the cell bodies. Recommended concentration, 2-3ug/ml. This antibody was successfully used on Human epithelial ovarian carcinoma: Abubaker et al. Mol Cancer.2009 Jul 28;8:51. (PMID: 19638206).
Western Blot: Preliminary experiments in lysates of cell lines A431, HeLa and Jurkat gave no specific signal but low background (at antibody concentration up to 1ug/ml).
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| Immunohistochemistry (IHC) of anti-PIK3CA antibody
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(2.5ug/ml) staining of paraffin embedded Human Brain (Cerebellar Cortex). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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| NCBI/Uniprot data below describe general gene information for PIK3CA. It may not necessarily be applicable to this product. |
| NCBI GI #
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54792082
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| NCBI GeneID
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5290
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| NCBI Accession #
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NP_006209.2
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[Other Products]
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| NCBI GenBank Nucleotide #
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NM_006218.3
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[Other Products]
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| UniProt Secondary Accession #
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Q14CW1; Q99762
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[Other Products]
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| UniProt Related Accession #
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P42336
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[Other Products]
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| Molecular Weight
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124,284 Da
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TOP
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| NCBI Official Full Name
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phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
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| NCBI Official Synonym Full Names
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phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha
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| NCBI Official Symbol
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PIK3CA
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[Similar Products]
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| NCBI Official Synonym Symbols
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MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; PI3K-alpha; p110-alpha
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[Similar Products]
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| NCBI Protein Information
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phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
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| UniProt Protein Name
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Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
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| UniProt Synonym Protein Names
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Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha; PtdIns-3-kinase subunit p110-alpha; p110alpha; Phosphoinositide-3-kinase catalytic alpha polypeptide; Serine/threonine protein kinase PIK3CA (EC:2.7.11.1
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| Protein Family
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Phosphatidylinositol 4,5-bisphosphate 3-kinase
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| UniProt Gene Name
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PIK3CA
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[Similar Products]
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| UniProt Synonym Gene Names
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PI3-kinase subunit alpha; PI3K-alpha; PI3Kalpha; PtdIns-3-kinase subunit alpha; PtdIns-3-kinase subunit p110-alpha; p110alpha
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[Similar Products]
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| UniProt Entry Name
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PK3CA_HUMAN
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| NCBI Summary for PIK3CA
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Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. A pseudogene of this gene has been defined on chromosome 22. [provided by RefSeq, Apr 2016]
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| UniProt Comments for PIK3CA
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PIK3CA: Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4- phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5- bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. Involved in signaling via insulin-receptor substrate (IRS) proteins. Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. Required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF. Regulates invadopodia formation in breast cancer cells through the PDPK1- AKT1 pathway. Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway. Has also serine-protein kinase activity: phosphorylates PIK3R1 (p85alpha regulatory subunit), EIF4EBP1 and HRAS. Heterodimer of a catalytic subunit PIK3CA and a p85 regulatory subunit (PIK3R1, PIK3R2 or PIK3R3). Interacts with IRS1 in nuclear extracts. Interacts with RUFY3. Interacts with RASD2. Interacts with APPL1. Interacts with HRAS1 and KRAS. Interaction with HRAS1/KRAS is required for PI3K pathway signaling and cell proliferation stimulated by EGF and FGF2. Belongs to the PI3/PI4-kinase family.
Protein type: Motility/polarity/chemotaxis; EC 2.7.11.1; EC 2.7.1.153; Oncoprotein; Kinase, lipid; Carbohydrate Metabolism - inositol phosphate
Chromosomal Location of Human Ortholog: 3q26.3
Cellular Component: cytosol; lamellipodium; phosphoinositide 3-kinase complex; plasma membrane
Molecular Function: 1-phosphatidylinositol-3-kinase activity; ATP binding; insulin receptor substrate binding; kinase activity; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; phosphatidylinositol-4-phosphate 3-kinase activity; phosphoinositide 3-kinase activity; protein binding; protein kinase activator activity; protein serine/threonine kinase activity
Biological Process: activation of protein kinase activity; angiogenesis; cardiac muscle contraction; endothelial cell migration; epidermal growth factor receptor signaling pathway; glucose metabolic process; leukocyte migration; liver development; negative regulation of neuron apoptosis; phosphatidylinositol biosynthetic process; phosphoinositide phosphorylation; phosphoinositide-mediated signaling; platelet activation; positive regulation of peptidyl-serine phosphorylation; protein amino acid phosphorylation; protein kinase B signaling cascade; regulation of cellular respiration; regulation of gene expression; regulation of multicellular organism growth; regulation of phosphoinositide 3-kinase cascade; T cell costimulation; T cell receptor signaling pathway; vascular endothelial growth factor receptor signaling pathway; vasculature development
Disease: Breast Cancer; Colorectal Cancer; Congenital Lipomatous Overgrowth, Vascular Malformations, And Epidermal Nevi; Cowden Syndrome 5; Gastric Cancer; Hepatocellular Carcinoma; Keratosis, Seborrheic; Lung Cancer; Megalencephaly-capillary Malformation-polymicrogyria Syndrome; Nevus, Epidermal; Ovarian Cancer
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| Research Articles on PIK3CA
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1. Data show that microRNA miR-451 target tuberous sclerosis 1 (TSC1) gene and activates the PI3-kinase/Akt protein/mTOR protein signaling in multiple myeloma (MM) Side population (SP) cells.
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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| Products associated with anti-PIK3CA antibody | Pathways associated with anti-PIK3CA antibody |
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| Diseases associated with anti-PIK3CA antibody | Organs/Tissues associated with anti-PIK3CA antibody |
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Antibody 
