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anti-PIK3CA antibody :: Goat PIK3CA/P110alpha Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS421100
Immunohistochemistry (IHC)
Unit / Price
0.1 mg  /  $300 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

PIK3CA/P110alpha, Polyclonal Antibody

★Popular Item★
 Also Known As   

Goat anti-PIK3CA/P110alpha Antibody

 Product Synonym Names    PIK3CA; p110-alpha; phosphoinositide-3-kinase, catalytic, alpha polypeptide; MGC142161; MGC142163; PI3K; PI3-kinase p110 subunit alpha; PtdIns-3-kinase p110; phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha; phosphatidylinositol 3-kinase, catalyti; PIK3CA antibody; p110-alpha antibody; phosphoinositide-3-kinase; catalytic; alpha polypeptide antibody; MGC142161 antibody; MGC142163 antibody; PI3K antibody; PI3-kinase p110 subunit alpha antibody; PtdIns-3-kinase p110 antibody; phosphatidylinositol 3-kinase; catalytic; 110-KD; alpha antibody; phosphatidylinositol 3-kinase; catalytic; alpha polypeptide antibody; phosphatidylinositol-4; 5-bisphosphate 3-kinase catalytic subunit; alpha isoform antibody; PIK3CA / P110alpha
 Product Gene Name   

anti-PIK3CA antibody

[Similar Products]
 Product Synonym Gene Name    P110alpha [Similar Products]
 Antibody/Peptide Pairs    PIK3CA / P110alpha peptide (MBS426574) is used for blocking the activity of PIK3CA/P110alpha antibody (MBS421100)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence    HQWLKDKNKG EIYD
 OMIM    114480
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 Clonality    Polyclonal
 Host    Goat
 Species Reactivity    Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
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 Purity/Purification    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
 Form/Format    Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
 Concentration    100ug specific antibody in 200ul (lot specific)
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 Immunogen    Peptide with sequence C-HQWLKDKNKGEIYD, from the internal region of the protein sequence according to NP_006209.2.
 Epitope    Internal region
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 Preparation and Storage    Aliquot and store at -20 degree C. Minimize freezing and thawing.
 ISO Certification    Manufactured in an ISO 9001:2015 Certified Laboratory.
 Other Notes    Small volumes of anti-PIK3CA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-PIK3CA antibody   

Peptide ELISA (EIA), Immunohistochemistry (IHC)

 Application Notes for anti-PIK3CA antibody    Peptide ELISA: Antibody detection limit dilution 1: 128000.
Immunohistochemistry: In paraffin embedded Human Brain (Cerebral Cortex) shows cytoplasm stained in the cell bodies. Recommended concentration, 2-3ug/ml. This antibody was successfully used on Human epithelial ovarian carcinoma: Abubaker et al. Mol Cancer.2009 Jul 28;8:51. (PMID: 19638206).
Western Blot: Preliminary experiments in lysates of cell lines A431, HeLa and Jurkat gave no specific signal but low background (at antibody concentration up to 1ug/ml).
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 Immunohistochemistry (IHC) of anti-PIK3CA antibody    (2.5ug/ml) staining of paraffin embedded Human Brain (Cerebellar Cortex). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
anti-PIK3CA antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for PIK3CA. It may not necessarily be applicable to this product.
 NCBI GI #    54792082
 NCBI GeneID    5290
 NCBI Accession #    NP_006209.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_006218.3 [Other Products]
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 UniProt Secondary Accession #    Q14CW1; Q99762 [Other Products]
 UniProt Related Accession #    P42336 [Other Products]
 Molecular Weight    124,284 Da
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 NCBI Official Full Name    phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
 NCBI Official Synonym Full Names    phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha
 NCBI Official Symbol    PIK3CA [Similar Products]
 NCBI Official Synonym Symbols   
MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; PI3K-alpha; p110-alpha
[Similar Products]
 NCBI Protein Information    phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
 UniProt Protein Name    Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
 UniProt Synonym Protein Names   
Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha; PtdIns-3-kinase subunit p110-alpha; p110alpha; Phosphoinositide-3-kinase catalytic alpha polypeptide; Serine/threonine protein kinase PIK3CA (EC:2.7.11.1
 Protein Family    Phosphatidylinositol 4,5-bisphosphate 3-kinase
 UniProt Gene Name    PIK3CA [Similar Products]
 UniProt Synonym Gene Names    PI3-kinase subunit alpha; PI3K-alpha; PI3Kalpha; PtdIns-3-kinase subunit alpha; PtdIns-3-kinase subunit p110-alpha; p110alpha [Similar Products]
 UniProt Entry Name    PK3CA_HUMAN
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 NCBI Summary for PIK3CA    Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. A pseudogene of this gene has been defined on chromosome 22. [provided by RefSeq, Apr 2016]
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 UniProt Comments for PIK3CA    PIK3CA: Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4- phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5- bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. Involved in signaling via insulin-receptor substrate (IRS) proteins. Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. Required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF. Regulates invadopodia formation in breast cancer cells through the PDPK1- AKT1 pathway. Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway. Has also serine-protein kinase activity: phosphorylates PIK3R1 (p85alpha regulatory subunit), EIF4EBP1 and HRAS. Heterodimer of a catalytic subunit PIK3CA and a p85 regulatory subunit (PIK3R1, PIK3R2 or PIK3R3). Interacts with IRS1 in nuclear extracts. Interacts with RUFY3. Interacts with RASD2. Interacts with APPL1. Interacts with HRAS1 and KRAS. Interaction with HRAS1/KRAS is required for PI3K pathway signaling and cell proliferation stimulated by EGF and FGF2. Belongs to the PI3/PI4-kinase family.

Protein type: Motility/polarity/chemotaxis; EC 2.7.11.1; EC 2.7.1.153; Oncoprotein; Kinase, lipid; Carbohydrate Metabolism - inositol phosphate

Chromosomal Location of Human Ortholog: 3q26.3

Cellular Component: cytosol; lamellipodium; phosphoinositide 3-kinase complex; plasma membrane

Molecular Function: 1-phosphatidylinositol-3-kinase activity; ATP binding; insulin receptor substrate binding; kinase activity; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; phosphatidylinositol-4-phosphate 3-kinase activity; phosphoinositide 3-kinase activity; protein binding; protein kinase activator activity; protein serine/threonine kinase activity

Biological Process: activation of protein kinase activity; angiogenesis; cardiac muscle contraction; endothelial cell migration; epidermal growth factor receptor signaling pathway; glucose metabolic process; leukocyte migration; liver development; negative regulation of neuron apoptosis; phosphatidylinositol biosynthetic process; phosphoinositide phosphorylation; phosphoinositide-mediated signaling; platelet activation; positive regulation of peptidyl-serine phosphorylation; protein amino acid phosphorylation; protein kinase B signaling cascade; regulation of cellular respiration; regulation of gene expression; regulation of multicellular organism growth; regulation of phosphoinositide 3-kinase cascade; T cell costimulation; T cell receptor signaling pathway; vascular endothelial growth factor receptor signaling pathway; vasculature development

Disease: Breast Cancer; Colorectal Cancer; Congenital Lipomatous Overgrowth, Vascular Malformations, And Epidermal Nevi; Cowden Syndrome 5; Gastric Cancer; Hepatocellular Carcinoma; Keratosis, Seborrheic; Lung Cancer; Megalencephaly-capillary Malformation-polymicrogyria Syndrome; Nevus, Epidermal; Ovarian Cancer
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 Research Articles on PIK3CA    1. Data show that microRNA miR-451 target tuberous sclerosis 1 (TSC1) gene and activates the PI3-kinase/Akt protein/mTOR protein signaling in multiple myeloma (MM) Side population (SP) cells.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-PIK3CA antibodyPathways associated with anti-PIK3CA antibody
 Reference Product  PubMed Publications
 KRAS antibody  >1031 publications with PIK3CA and KRAS
 PTEN antibody  >760 publications with PIK3CA and PTEN
 NRAS antibody  >315 publications with PIK3CA and NRAS
 AKT1 antibody  >286 publications with PIK3CA and AKT1
 HRAS antibody  >131 publications with PIK3CA and HRAS
 PIK3R1 antibody  >62 publications with PIK3CA and PIK3R1
 IRS1 antibody  >11 publications with PIK3CA and IRS1
 IRS2 antibody  >8 publications with PIK3CA and IRS2
 Products by Pathway  Pathway Diagram
 3-phosphoinositide Biosynthesis Pathway antibodies  3-phosphoinositide Biosynthesis Pathway Diagram
 3-phosphoinositide Biosynthesis Pathway antibodies  3-phosphoinositide Biosynthesis Pathway Diagram
 AGE-RAGE Signaling Pathway In Diabetic Complications antibodies  AGE-RAGE Signaling Pathway In Diabetic Complications Diagram
 AGE-RAGE Signaling Pathway In Diabetic Complications antibodies  AGE-RAGE Signaling Pathway In Diabetic Complications Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
Diseases associated with anti-PIK3CA antibodyOrgans/Tissues associated with anti-PIK3CA antibody
 Disease Name  Pubmed Publications
 Adenocarcinoma Antibodies  >482 publications with PIK3CA and Adenocarcinoma
 Colorectal Neoplasms Antibodies  >451 publications with PIK3CA and Colorectal Neoplasms
 Breast Neoplasms Antibodies  >398 publications with PIK3CA and Breast Neoplasms
 Lung Neoplasms Antibodies  >259 publications with PIK3CA and Lung Neoplasms
 Carcinoma, Squamous Cell Antibodies  >241 publications with PIK3CA and Carcinoma, Squamous Cell
 Neoplasm Metastasis Antibodies  >226 publications with PIK3CA and Neoplasm Metastasis
 Neoplasms, Experimental Antibodies  >202 publications with PIK3CA and Neoplasms, Experimental
 Sarcoma Antibodies  >96 publications with PIK3CA and Sarcoma
 Liver Neoplasms Antibodies  >80 publications with PIK3CA and Liver Neoplasms
 Neoplasm Invasiveness Antibodies  >80 publications with PIK3CA and Neoplasm Invasiveness
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >364 publications with PIK3CA and Lung
 Blood Antibodies  >145 publications with PIK3CA and Blood
 Lymph Node Antibodies  >112 publications with PIK3CA and Lymph Node
 Liver Antibodies  >110 publications with PIK3CA and Liver
 Brain Antibodies  >96 publications with PIK3CA and Brain
 Vascular Antibodies  >77 publications with PIK3CA and Vascular
 Prostate Antibodies  >36 publications with PIK3CA and Prostate
 Ovary Antibodies  >29 publications with PIK3CA and Ovary
 Heart Antibodies  >24 publications with PIK3CA and Heart
 Kidney Antibodies  >18 publications with PIK3CA and Kidney
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