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anti-PIWIL1 antibody :: Rabbit PIWI-L1 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS150841
Unit / Price
0.1 mg  /  $365 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

PIWI-L1 (PIWIL1), Polyclonal Antibody

 Also Known As   

PIWI-L1 Antibody

 Product Synonym Names    PIWI-L1; HIWI; MIWI; PIWI; CT80.1; HIWI; Piwi-like protein 1; piwi-like 1 (Drosophila)
 Product Gene Name   

anti-PIWIL1 antibody

[Similar Products]
 Antibody/Peptide Pairs    PIWI-L1 peptide (MBS152665) is used for blocking the activity of PIWI-L1 antibody (MBS150841)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    605571
 3D Structure    ModBase 3D Structure for Q96J94
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human, Mouse, Rat
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 Purity/Purification    PIWI-L1 Antibody is affinity chromatography purified via peptide column.
 Form/Format    Liquid
 Concentration    1 mg/mL (lot specific)
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 Conjugate    Unconjugated
 Immunogen    PIWI-L1 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human PIWI-L1.
 Buffer    PIWI-L1 Antibody is supplied in PBS containing 0.02% sodium azide.
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 Preparation and Storage    PIWI-L1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
 Other Notes    Small volumes of anti-PIWIL1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-PIWIL1 antibody

   PIWI-L1 Antibody: PIWI-L1 is a member of the PIWI subfamily of Argonaute proteins, evolutionarily conserved proteins containing both PAZ and Piwi motifs that are crucial for the biogenesis and function of small non-coding RNAs and play important roles in stem cell self-renewal, RNA silencing, and translational regulation in diverse organisms. PIWI-L1 is thought to play a role as an intrinsic regulator of the self-renewal capacity of germline and hematopoietic stem cells as removing PIWI-L1 protein from single germline stem cells significantly decreases the rate of their division. Recent studies have shown that overexpression of PIWI-L1 (as well as other PIWI-like proteins) are potential biomarkers for colon and other cancers.
 Applications Tested/Suitable for anti-PIWIL1 antibody   

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

 Application Notes for anti-PIWIL1 antibody    PIWI-L1 antibody can be used for detection of PIWI-L1 by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL.
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 Western Blot (WB) of anti-PIWIL1 antibody    Western blot analysis of PIWI-L1 in HepG2 cell lysate with PIWI-L1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
anti-PIWIL1 antibody Western Blot (WB) (WB) image
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 Immunofluorescence (IF) of anti-PIWIL1 antibody    Immunofluorescence of PIWI-L1 in rat liver tissue with PIWI-L1 antibody at 20 μg/mL.
anti-PIWIL1 antibody Immunofluorescence (IF) image
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 Immunohistochemistry (IHC) of anti-PIWIL1 antibody    Immunohistochemistry of PIWI-L1 in rat liver tissue with PIWI-L1 antibody at 10 μg/mL.
anti-PIWIL1 antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for PIWIL1. It may not necessarily be applicable to this product.
 NCBI GI #    14579645
 NCBI GeneID    9271
 NCBI Accession #    AAK69348 [Other Products]
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 UniProt Primary Accession #    Q96J94 [Other Products]
 UniProt Secondary Accession #    O95404; Q8NA60; Q8TBY5; Q96JD5; A4F266 [Other Products]
 UniProt Related Accession #    Q96J94 [Other Products]
 Molecular Weight    89,484 Da
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 NCBI Official Full Name    PIWI protein
 NCBI Official Synonym Full Names    piwi-like RNA-mediated gene silencing 1
 NCBI Official Symbol    PIWIL1 [Similar Products]
 NCBI Official Synonym Symbols   
HIWI; MIWI; PIWI; CT80.1
[Similar Products]
 NCBI Protein Information    piwi-like protein 1; piwi homolog
 UniProt Protein Name    Piwi-like protein 1
 Protein Family    Piwi-like protein
 UniProt Gene Name    PIWIL1 [Similar Products]
 UniProt Synonym Gene Names    HIWI [Similar Products]
 UniProt Entry Name    PIWL1_HUMAN
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 NCBI Summary for PIWIL1    This gene encodes a member of the PIWI subfamily of Argonaute proteins, evolutionarily conserved proteins containing both PAZ and Piwi motifs that play important roles in stem cell self-renewal, RNA silencing, and translational regulation in diverse organisms. The encoded protein may play a role as an intrinsic regulator of the self-renewal capacity of germline and hematopoietic stem cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]
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 UniProt Comments for PIWIL1    PIWIL1: Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds methylated piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer- independent mechanism and are primarily derived from transposons and other repeated sequence elements. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Probable component of some RISC complex, which mediates RNA cleavage and translational silencing. Also plays a role in the formation of chromatoid bodies and is required for some miRNAs stability. Isoform 3 may be a negative developmental regulator. Interacts (via Piwi domain) with DICER1, suggesting that it forms ribonucleoprotein RISC complexes. This interaction is regulated by HSP90AB1 activity. Interacts with MAEL, KIF17, PABPC1, PRMT5 and WDR77. Interacts (when methylated on arginine residues) with TDRD1, TDRKH/TDRD2, RNF17/TDRD4, TDRD6, TDRD7 and TDRD9. Isoform 3 is down-regulated in CD34(+) hematopoietic cells during differentiation. Detected in most fetal and adult tissues. Expressed in testes, specifically in germline cells; detected in spermatocytes and spermatids during spermatogenesis. Increased expression in testicular tumors originating from embryonic germ cells with retention of germ cells phenotype. No expression in testicular tumors of somatic origin, such as Sertoli cell and Leydig cell tumors. Overexpressed in gastric cancer cells. Isoform 3 is ubiquitously expressed, and specifically in CD34+ hematopoietic progenitor cells but not in more differentiated cells. Belongs to the argonaute family. Piwi subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, peripheral

Chromosomal Location of Human Ortholog: 12q24.33

Cellular Component: polysome; mRNA cap complex; cytoplasm

Molecular Function: mRNA binding; protein binding; single-stranded RNA binding; protein kinase binding

Biological Process: regulation of translation; meiotic cell cycle; multicellular organismal development; gene expression; RNA-mediated gene silencing; spermatid development
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 Research Articles on PIWIL1    1. A important role of HIWI and DPPA2 in tumorigenesis and also in lymph node metastasis of tumor cells.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-PIWIL1 antibodyPathways associated with anti-PIWIL1 antibody
 Reference Product  PubMed Publications
 TDRD1 antibody  >9 publications with PIWIL1 and TDRD1
 MAEL antibody  >3 publications with PIWIL1 and MAEL
 TDRKH antibody  >2 publications with PIWIL1 and TDRKH
 CREM antibody  >2 publications with PIWIL1 and CREM
 MOV10L1 antibody  >2 publications with PIWIL1 and MOV10L1
 DICER1 antibody  >2 publications with PIWIL1 and DICER1
 SND1 antibody  >1 publications with PIWIL1 and SND1
 Products by Pathway  Pathway Diagram
 Dorso-ventral Axis Formation Pathway antibodies  Dorso-ventral Axis Formation Pathway Diagram
 Dorso-ventral Axis Formation Pathway antibodies  Dorso-ventral Axis Formation Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 PIWI-interacting RNA (piRNA) Biogenesis Pathway antibodies  PIWI-interacting RNA (piRNA) Biogenesis Pathway Diagram
 Regulatory RNA Pathways antibodies  Regulatory RNA Pathways Diagram
Diseases associated with anti-PIWIL1 antibodyOrgans/Tissues associated with anti-PIWIL1 antibody
 Disease Name  Pubmed Publications
 Infertility, Male Antibodies  >9 publications with PIWIL1 and Infertility, Male
 Adenocarcinoma Antibodies  >8 publications with PIWIL1 and Adenocarcinoma
 Neoplasm Metastasis Antibodies  >8 publications with PIWIL1 and Neoplasm Metastasis
 Ovarian Neoplasms Antibodies  >2 publications with PIWIL1 and Ovarian Neoplasms
 Gonadal Disorders Antibodies  >2 publications with PIWIL1 and Gonadal Disorders
 Uterine Cervical Neoplasms Antibodies  >2 publications with PIWIL1 and Uterine Cervical Neoplasms
 Hyperplasia Antibodies  >1 publications with PIWIL1 and Hyperplasia
 Fetal Weight Antibodies  >1 publications with PIWIL1 and Fetal Weight
 Fetal Death Antibodies  >1 publications with PIWIL1 and Fetal Death
 Prostatic Diseases Antibodies  >1 publications with PIWIL1 and Prostatic Diseases
 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >47 publications with PIWIL1 and Testis
 Brain Antibodies  >4 publications with PIWIL1 and Brain
 Uterus Antibodies  >1 publications with PIWIL1 and Uterus
 Muscle Antibodies  >1 publications with PIWIL1 and Muscle
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