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anti-POU2F1 antibody :: Goat POU2F1/OCT1 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS422485
Western Blot (WB)
Unit / Price
0.1 mg  /  $300 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

POU2F1/OCT1, Polyclonal Antibody

★Popular Item★
 Also Known As   

Goat anti-POU2F1/OCT1 Antibody

 Product Synonym Names    POU2F1; POU class 2 homeobox 1; OCT1; OTF1; OTTHUMP00000032348; Octamer-binding transcription factor-1; POU domain, class 2, transcription factor 1; OCT1 antibody; Octamer-binding transcription factor-1 antibody; OTF1 antibody; OTTHUMP00000032348 antibody; POU class 2 homeobox 1 antibody; POU domain; class 2; transcription factor 1 antibody; POU2F1 antibody; POU2F1 / OCT1
 Product Gene Name   

anti-POU2F1 antibody

[Similar Products]
 Product Synonym Gene Name    OCT1 [Similar Products]
 Antibody/Peptide Pairs    POU2F1 / OCT1 peptide (MBS427744) is used for blocking the activity of POU2F1/OCT1 antibody (MBS422485)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence    NVQSKSNEES GDSQQ
 OMIM    164175
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 Clonality    Polyclonal
 Host    Goat
 Species Reactivity    Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Pig
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 Purity/Purification    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
 Form/Format    Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
 Concentration    100ug specific antibody in 200ul (lot specific)
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 Immunogen    Peptide with sequence C-NVQSKSNEESGDSQQ, from the internal region (near N Terminus) of the protein sequence according to NP_002688.2.
 Epitope    Internal region (near N Terminus)
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 Preparation and Storage    Aliquot and store at -20 degree C. Minimize freezing and thawing.
 ISO Certification    Manufactured in an ISO 9001:2015 Certified Laboratory.
 Other Notes    Small volumes of anti-POU2F1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-POU2F1 antibody   

Peptide ELISA (EIA), Western Blot (WB)

 Application Notes for anti-POU2F1 antibody    Peptide ELISA: Antibody detection limit dilution 1: 4000.
Western Blot: Approx 100kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 76.5kDa according to NP_002688.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.5-2ug/ml.
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 Western Blot (WB) of anti-POU2F1 antibody    (0.5ug/ml) staining of nuclear HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-POU2F1 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for POU2F1. It may not necessarily be applicable to this product.
 NCBI GI #    42476164
 NCBI GeneID    5451
 NCBI Accession #    NP_002688.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_002697.2 [Other Products]
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 UniProt Secondary Accession #    Q5TBT7; Q6PK46; Q8NEU9; Q9BPV1; B1AL91; B1AL93; B4E029; J3KP77 [Other Products]
 UniProt Related Accession #    P14859 [Other Products]
 Molecular Weight    72,082 Da
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 NCBI Official Full Name    POU domain, class 2, transcription factor 1
 NCBI Official Synonym Full Names    POU class 2 homeobox 1
 NCBI Official Symbol    POU2F1 [Similar Products]
 NCBI Official Synonym Symbols   
OCT1; OTF1; oct-1B
[Similar Products]
 NCBI Protein Information    POU domain, class 2, transcription factor 1
 UniProt Protein Name    POU domain, class 2, transcription factor 1
 UniProt Synonym Protein Names   
NF-A1; Octamer-binding protein 1; Oct-1; Octamer-binding transcription factor 1; OTF-1
 Protein Family    POU domain, class 2, transcription factor
 UniProt Gene Name    POU2F1 [Similar Products]
 UniProt Synonym Gene Names    OCT1; OTF1; Oct-1; OTF-1 [Similar Products]
 UniProt Entry Name    PO2F1_HUMAN
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 NCBI Summary for POU2F1    The OCT1 transcription factor was among the first identified members of the POU transcription factor family (summarized by Sturm et al., 1993 [PubMed 8314572]). Members of this family contain the POU domain, a 160-amino acid region necessary for DNA binding to the octameric sequence ATGCAAAT.[supplied by OMIM, Jul 2010]
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 UniProt Comments for POU2F1    Oct1: a ubiquitous transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR. Phosphorylation apparently inhibits its interaction with DNA. Four splice variant isoforms have been described. Isoform 2 is lymphocyte specific.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 1q24.2

Cellular Component: endoplasmic reticulum; intracellular membrane-bound organelle; nucleoplasm; nucleus

Molecular Function: protein binding; sequence-specific DNA binding

Biological Process: negative regulation of transcription, DNA-dependent; snRNA transcription from RNA polymerase II promoter
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 Research Articles on POU2F1    1. OCT1 siRNA significantly reduced the proliferation rate of SW620 and LoVo cells, inhibited the migration and invasion, and could reverse EMT in these two CRC cells, indicating that OCT1 plays a critical role in Colorectal cancer progression
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-POU2F1 antibodyPathways associated with anti-POU2F1 antibody
 Reference Product  PubMed Publications
 HCFC1 antibody  >388 publications with POU2F1 and HCFC1
 SP1 antibody  >42 publications with POU2F1 and SP1
 POU2AF1 antibody  >28 publications with POU2F1 and POU2AF1
 NF1 antibody  >7 publications with POU2F1 and NF1
 SOX2 antibody  >7 publications with POU2F1 and SOX2
 TBP antibody  >4 publications with POU2F1 and TBP
 BRCA1 antibody  >4 publications with POU2F1 and BRCA1
 SNAPC4 antibody  >2 publications with POU2F1 and SNAPC4
 PRKDC antibody  >1 publications with POU2F1 and PRKDC
 Products by Pathway  Pathway Diagram
 ATF-2 Transcription Factor Network Pathway antibodies  ATF-2 Transcription Factor Network Pathway Diagram
 Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies  Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
 Calcium Signaling In The CD4+ TCR Pathway antibodies  Calcium Signaling In The CD4+ TCR Pathway Diagram
 FOXA1 Transcription Factor Network Pathway antibodies  FOXA1 Transcription Factor Network Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Glucocorticoid Receptor Regulatory Network Pathway antibodies  Glucocorticoid Receptor Regulatory Network Pathway Diagram
 RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway antibodies  RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway Diagram
 RNA Polymerase III Abortive And Retractive Initiation Pathway antibodies  RNA Polymerase III Abortive And Retractive Initiation Pathway Diagram
 RNA Polymerase III Transcription Pathway antibodies  RNA Polymerase III Transcription Pathway Diagram
 RNA Polymerase III Transcription Initiation Pathway antibodies  RNA Polymerase III Transcription Initiation Pathway Diagram
Diseases associated with anti-POU2F1 antibodyOrgans/Tissues associated with anti-POU2F1 antibody
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >15 publications with POU2F1 and Necrosis
 Inflammation Antibodies  >13 publications with POU2F1 and Inflammation
 Nervous System Diseases Antibodies  >10 publications with POU2F1 and Nervous System Diseases
 Kidney Diseases Antibodies  >7 publications with POU2F1 and Kidney Diseases
 Lung Diseases Antibodies  >6 publications with POU2F1 and Lung Diseases
 Liver Diseases Antibodies  >5 publications with POU2F1 and Liver Diseases
 Brain Diseases Antibodies  >5 publications with POU2F1 and Brain Diseases
 Carcinoma, Hepatocellular Antibodies  >4 publications with POU2F1 and Carcinoma, Hepatocellular
 Liver Neoplasms Antibodies  >3 publications with POU2F1 and Liver Neoplasms
 Cholestasis Antibodies  >3 publications with POU2F1 and Cholestasis
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >134 publications with POU2F1 and Blood
 Liver Antibodies  >42 publications with POU2F1 and Liver
 Kidney Antibodies  >36 publications with POU2F1 and Kidney
 Brain Antibodies  >33 publications with POU2F1 and Brain
 Embryonic Tissue Antibodies  >18 publications with POU2F1 and Embryonic Tissue
 Vascular Antibodies  >14 publications with POU2F1 and Vascular
 Lung Antibodies  >14 publications with POU2F1 and Lung
 Spleen Antibodies  >12 publications with POU2F1 and Spleen
 Muscle Antibodies  >11 publications with POU2F1 and Muscle
 Thymus Antibodies  >8 publications with POU2F1 and Thymus
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