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anti-PRKAA1 antibody :: Rabbit PRKAA1 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS127673
Western Blot (WB)
Unit / Price
0.05 mL  /  $230 +1 FREE 8GB USB
0.1 mL  /  $305 +1 FREE 8GB USB
0.2 mL  /  $460 +1 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

PRKAA1, Polyclonal Antibody

★Popular Item★
 Also Known As   

PRKAA1 Polyclonal Antibody

 Product Synonym Names    AMPK: PRKAA1; AMPK; AMPKa1; MGC33776; MGC57364
 Product Gene Name   

anti-PRKAA1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    602739
 3D Structure    ModBase 3D Structure for Q13131
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human, Mouse, Rat
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 Purity/Purification    Affinity Purification
 Concentration    1mg/ml (lot specific)
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 Species    Human
 Immunogen    Recombinant Protein
 Immunogen    Recombinant protein of human PRKAA1
 Calculated Molecular Weight    63kDa
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 Preparation and Storage    Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
 Other Notes    Small volumes of anti-PRKAA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-PRKAA1 antibody

   AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals and plays a key role in the regulation of energy homeostasis (1). AMPK is a heterotrimeric complex composed of a catalytic alpha subunit and regulatory beta and gamma subunits, each of which is encoded by two or three distinct genes (alpha1, 2; beta1, 2; gamma1, 2, 3) (2). The kinase is activated by an elevated AMP/ATP ratio due to cellular and environmental stress, such as heat shock, hypoxia, and ischemia (1). The tumor suppressor LKB1, in association with accessory proteins STRAD and MO25, phosphorylates AMPKalpha at Thr172 in the activation loop, and this phosphorylation is required for AMPK activation (3-5). AMPKalpha is also phosphorylated at Thr258 and Ser485 (for alpha1; Ser491 for alpha2). The upstream kinase and the biological significance of these phosphorylation events have yet to be elucidated (6). The beta1 subunit is post-translationally modified by myristoylation and multi-site phosphorylation including Ser24/25, Ser96, Ser101, Ser108, and Ser182 (6,7). Phosphorylation at Ser108 of the beta1 subunit seems to be required for the activation of AMPK enzyme, while phosphorylation at Ser24/25 and Ser182 affects AMPK localization (7). Several mutations in AMPKgamma subunits have been identified, most of which are located in the putative AMP/ATP binding sites (CBS or Bateman domains). Mutations at these sites lead to reduction of AMPK activity and cause glycogen accumulation in heart or skeletal muscle (1,2). Accumulating evidence indicates that AMPK not only regulates the metabolism of fatty acids and glycogen, but also modulates protein synthesis and cell growth through EF2 and TSC2/mTOR pathways, as well as blood flow via eNOS/nNOS (1).
 Product Categories/Family for anti-PRKAA1 antibody    Polyclonal
 Applications Tested/Suitable for anti-PRKAA1 antibody   

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

 Application Notes for anti-PRKAA1 antibody    WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:20 - 1:100
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 Western Blot (WB) of anti-PRKAA1 antibody    Western blot analysis of extracts of various cell lines, using PRKAA1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
anti-PRKAA1 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for PRKAA1. It may not necessarily be applicable to this product.
 NCBI GI #    254763436
 NCBI GeneID    5562
 NCBI Accession #    Q13131.4 [Other Products]
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 UniProt Primary Accession #    Q13131 [Other Products]
 UniProt Secondary Accession #    O00286; Q5D0E1; Q86VS1; Q9UNQ4; A8MTQ6; B2R7E1 [Other Products]
 UniProt Related Accession #    Q13131 [Other Products]
 Molecular Weight    559
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 NCBI Official Full Name    5'-AMP-activated protein kinase catalytic subunit alpha-1
 NCBI Official Synonym Full Names    protein kinase, AMP-activated, alpha 1 catalytic subunit
 NCBI Official Symbol    PRKAA1 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    5'-AMP-activated protein kinase catalytic subunit alpha-1; ACACA kinase; AMPK alpha 1; HMGCR kinase; AMPK subunit alpha-1; tau-protein kinase PRKAA1; acetyl-CoA carboxylase kinase; AMP -activate kinase alpha 1 subunit; hydroxymethylglutaryl-CoA reductase kinase; AMP-activated protein kinase, catalytic, alpha-1; 5'-AMP-activated protein kinase, catalytic alpha-1 chain
 UniProt Protein Name    5'-AMP-activated protein kinase catalytic subunit alpha-1
 UniProt Synonym Protein Names   
Acetyl-CoA carboxylase kinase (EC:; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase (EC:; HMGCR kinase; Tau-protein kinase PRKAA1 (EC:
 UniProt Gene Name    PRKAA1 [Similar Products]
 UniProt Synonym Gene Names    AMPK1; AMPK subunit alpha-1; ACACA kinase; HMGCR kinase [Similar Products]
 UniProt Entry Name    AAPK1_HUMAN
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 NCBI Summary for PRKAA1    The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
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 UniProt Comments for PRKAA1    AMPKA1: a catalytic subunit of AMP-activated protein kinase (AMPK). Acts as an energy sensor, playing a key role in regulating cellular energy metabolism. A protein kinase of the CAMKL family whose activation is regulated by the balance between ADP/AMP/ATP, and intracellular Ca(2+) levels. Acts as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-ADP and -AMP rise in response to fuel limitation and/or hypoxia. Activates energy-producing pathways and inhibits energy-consuming processes. Restores ATP levels in cells by switching off anabolic and switching on catabolic pathways. Activated primarily by rising ADP levels and not, as previously thought, solely by AMP. AMPK resembles an adenylate charge regulatory system in which anabolic and catabolic pathways are regulated by adenine nucleotide ratios. Acts via direct phosphorylation of a number of metabolic enzymes and transcription regulators. Activated by at least two distinct upstream kinases: the tumor suppressor LKB1 and CaMKK2. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton, probably by indirectly activating myosin. AMPK is a heterotrimer of an alpha catalytic subunit (AMPKA1 or -2), a beta (AMPKB1 or -2) and a gamma non-catalytic subunit (AMPKG1, -2 or -3). Different possible combinations of subunits give rise to 12 different holoenzymes. Binding of ADP or AMP to non-catalytic gamma subunit (PRKAG1, -2 or -3) results in allosteric activation. AMPK is activated by antihyperglycemic drug metformin, a drug prescribed to patients with type 2 diabetes: in vivo, metformin seems to mainly inhibit liver gluconeogenesis. However, metformin can be used to activate AMPK in muscle and other cells in culture or ex vivo. Selectively inhibited by compound C (6-[4-(2-Piperidin-1-yl-ethoxy)-phenyl)]-3-pyridin-4-yl-pyyrazolo[1,5-a] pyrimidine. Activated by resveratrol, a natural polyphenol present in red wine, and S17834, a synthetic polyphenol. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; Autophagy; EC; EC; EC; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; EC; CAMK group; CAMKL family; AMPK subfamily

Chromosomal Location of Human Ortholog: 5p12

Cellular Component: nucleoplasm; cytoplasm; apical plasma membrane; intracellular; nucleus; cytosol; AMP-activated protein kinase complex

Molecular Function: protein C-terminus binding; AMP-activated protein kinase activity; cAMP-dependent protein kinase activity; metal ion binding; [acetyl-CoA carboxylase] kinase activity; histone serine kinase activity; protein kinase activity; protein binding; tau-protein kinase activity; chromatin binding; kinase binding; ATP binding; [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity

Biological Process: protein heterooligomerization; activation of MAPK activity; lipid biosynthetic process; rhythmic process; positive regulation of cholesterol biosynthetic process; signal transduction; glucose homeostasis; protein amino acid phosphorylation; response to caffeine; cellular response to glucose starvation; cellular response to nutrient levels; negative regulation of lipid catabolic process; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; response to gamma radiation; cell cycle arrest; negative regulation of glucosylceramide biosynthetic process; fatty acid biosynthetic process; positive regulation of autophagy; response to UV; negative regulation of TOR signaling pathway; Wnt receptor signaling pathway; transcription, DNA-dependent; glucose metabolic process; regulation of circadian rhythm; cholesterol biosynthetic process; fatty acid homeostasis; cold acclimation; positive regulation of glycolysis; insulin receptor signaling pathway; response to hypoxia; autophagy; fatty acid oxidation; response to activity; negative regulation of apoptosis
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 Research Articles on PRKAA1    1. AMPK is downstream of VEGFR2 and inhibition of AMPK signaling abrogated decorin-evoked autophagy.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-PRKAA1 antibodyPathways associated with anti-PRKAA1 antibody
 Reference Product  PubMed Publications
 PRKAA2 antibody  >76 publications with PRKAA1 and PRKAA2
 STK11 antibody  >19 publications with PRKAA1 and STK11
 PRKAG1 antibody  >18 publications with PRKAA1 and PRKAG1
 PRKAB2 antibody  >17 publications with PRKAA1 and PRKAB2
 PRKAB1 antibody  >17 publications with PRKAA1 and PRKAB1
 PRKAG3 antibody  >14 publications with PRKAA1 and PRKAG3
 PRKAG2 antibody  >10 publications with PRKAA1 and PRKAG2
 TSC2 antibody  >2 publications with PRKAA1 and TSC2
 Products by Pathway  Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 Circadian Rhythm Pathway antibodies  Circadian Rhythm Pathway Diagram
 Circadian Rhythm Pathway antibodies  Circadian Rhythm Pathway Diagram
 Energy Metabolism Pathway antibodies  Energy Metabolism Pathway Diagram
 Energy Dependent Regulation Of MTOR By LKB1-AMPK Pathway antibodies  Energy Dependent Regulation Of MTOR By LKB1-AMPK Pathway Diagram
Diseases associated with anti-PRKAA1 antibodyOrgans/Tissues associated with anti-PRKAA1 antibody
 Disease Name  Pubmed Publications
 Death Antibodies  >13 publications with PRKAA1 and Death
 Cardiovascular Diseases Antibodies  >12 publications with PRKAA1 and Cardiovascular Diseases
 Fatty Liver Antibodies  >11 publications with PRKAA1 and Fatty Liver
 Inflammation Antibodies  >10 publications with PRKAA1 and Inflammation
 Heart Diseases Antibodies  >10 publications with PRKAA1 and Heart Diseases
 Necrosis Antibodies  >7 publications with PRKAA1 and Necrosis
 Nervous System Diseases Antibodies  >6 publications with PRKAA1 and Nervous System Diseases
 Insulin Resistance Antibodies  >6 publications with PRKAA1 and Insulin Resistance
 Liver Diseases Antibodies  >5 publications with PRKAA1 and Liver Diseases
 Hypertrophy Antibodies  >4 publications with PRKAA1 and Hypertrophy
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >51 publications with PRKAA1 and Muscle
 Blood Antibodies  >24 publications with PRKAA1 and Blood
 Liver Antibodies  >22 publications with PRKAA1 and Liver
 Vascular Antibodies  >18 publications with PRKAA1 and Vascular
 Adipose Tissue Antibodies  >15 publications with PRKAA1 and Adipose Tissue
 Brain Antibodies  >12 publications with PRKAA1 and Brain
 Lung Antibodies  >11 publications with PRKAA1 and Lung
 Connective Tissue Antibodies  >9 publications with PRKAA1 and Connective Tissue
 Pancreas Antibodies  >7 publications with PRKAA1 and Pancreas
 Nerve Antibodies  >6 publications with PRKAA1 and Nerve
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