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anti-PRKAA2 antibody :: Rabbit anti-Human PRKAA2 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS7045064
Unit / Price
0.05 mg  /  $160 +1 FREE 8GB USB
0.1 mg  /  $235 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

PRKAA2, Polyclonal Antibody

★Popular Item★
 Also Known As   

PRKAA2 Antibody

 Product Synonym Names    5'-AMP-activated protein kinase catalytic subunit alpha-2; Acetyl-CoA carboxylase kinase (EC:2.7.11.27)
 Product Gene Name   

anti-PRKAA2 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    600497
 3D Structure    ModBase 3D Structure for P54646
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human
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 Purity/Purification    >95%, Protein G purified
 Form/Format    Liquid
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 Species    Human
 Immunogen    Recombinant human 5'-AMP-activated protein kinase catalytic subunit alpha-2 protein
 Conjugate    Non-conjugated
 Buffer    Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
 Santa Cruz Alternative    Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-19131 / sc-19129
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 Preparation and Storage    Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of anti-PRKAA2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-PRKAA2 antibody

   Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3. Involved in insulin receptor/INSR internalization (PubMed:25687571). AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160. Regulates transcription and chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2, FOXO3, histone H2B, HDAC5, MEF2C, MLXIPL/ChREBP, EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A. Acts as a key regulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the cytoplasm. In response to stress, phosphorylates 'Ser-36' of histone H2B (H2BS36ph), leading to promote transcription. Acts as a key regulator of cell growth and proliferation by phosphorylating TSC2, RPTOR and ATG1/ULK1: in response to nutrient limitation, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2. In response to nutrient limitation, promotes autophagy by phosphorylating and activating ATG1/ULK1. AMPK also acts as a regulator of circadian rhythm by mediating phosphorylation of CRY1, leading to destabilize it. May regulate the Wnt signaling pathway by phosphorylating CTNNB1, leading to stabilize it. Also phosphorylates CFTR, EEF2K, KLC1, NOS3 and SLC12A1. Plays an important role in the differential regulation of pro-autophagy (composed of PIK3C3, BECN1, PIK3R4 and UVRAG or ATG14) and non-autophagy (composed of PIK3C3, BECN1 and PIK3R4) complexes, in response to glucose starvation. Can inhibit the non-autophagy complex by phosphorylating PIK3C3 and can activate the pro-autophagy complex by phosphorylating BECN1 (By similarity).
 Applications Tested/Suitable for anti-PRKAA2 antibody   

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

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 Immunohistochemistry (IHC) of anti-PRKAA2 antibody    Immunohistochemistry of paraffin-embedded human kidney tissue using MBS7045064 at dilution of 1:100
anti-PRKAA2 antibody Immunohistochemistry (IHC) (IHC) image
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 Immunofluorescence (IF) of anti-PRKAA2 antibody    Immunofluorescent analysis of MCF7 cells using MBS7045064 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
anti-PRKAA2 antibody Immunofluorescence (IF) image
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 Western Blot (WB) of anti-PRKAA2 antibody    Western Blot
Positive WB detected in:Hela whole cell lysate,Mouse heart tissue,Mouse lung tissue,Mouse kidney tissue,Mouse brain tissue,Mouse skeletal muscle tissue
All lanes: PRKAA2 antibody at 2.7ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 63 kDa
Observed band size: 63,48 kDa

anti-PRKAA2 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for PRKAA2. It may not necessarily be applicable to this product.
 NCBI GI #    46877068
 NCBI GeneID    5563
 NCBI Accession #    NP_006243.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_006252.3 [Other Products]
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 UniProt Primary Accession #    P54646 [Other Products]
 UniProt Secondary Accession #    Q9H1E8; Q9UD43 [Other Products]
 UniProt Related Accession #    P54646; AAB32732 [Other Products]
 Molecular Weight    62,320 Da
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 NCBI Official Full Name    5'-AMP-activated protein kinase catalytic subunit alpha-2
 NCBI Official Synonym Full Names    protein kinase AMP-activated catalytic subunit alpha 2
 NCBI Official Symbol    PRKAA2 [Similar Products]
 NCBI Official Synonym Symbols   
AMPK; AMPK2; PRKAA; AMPKa2
[Similar Products]
 NCBI Protein Information    5'-AMP-activated protein kinase catalytic subunit alpha-2
 UniProt Protein Name    5'-AMP-activated protein kinase catalytic subunit alpha-2
 UniProt Synonym Protein Names   
Acetyl-CoA carboxylase kinase (EC:2.7.11.27
 UniProt Gene Name    PRKAA2 [Similar Products]
 UniProt Synonym Gene Names    AMPK; AMPK2; HMGCR kinase [Similar Products]
 UniProt Entry Name    AAPK2_HUMAN
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 NCBI Summary for PRKAA2    The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. [provided by RefSeq, Jul 2008]
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 UniProt Comments for PRKAA2    AMPKA2: a catalytic subunit of AMP-activated protein kinase (AMPK). Acts as an energy sensor, playing a key role in regulating cellular energy metabolism. A protein kinase of the CAMKL family whose activation is regulated by the balance between ADP/AMP/ATP, and intracellular Ca(2+) levels. Acts as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-ADP and -AMP rise in response to fuel limitation and/or hypoxia. Activates energy-producing pathways and inhibits energy-consuming processes. Restores ATP levels in cells by switching off anabolic and switching on catabolic pathways. Activated primarily by rising ADP levels and not, as previously thought, solely by AMP. AMPK resembles an adenylate charge regulatory system in which anabolic and catabolic pathways are regulated by adenine nucleotide ratios. Acts via direct phosphorylation of metabolic enzymes and transcription regulators. Regulates fatty acid synthesis by phosphorylating acetyl-CoA carboxylase. Regulates cholesterol synthesis by phosphorylating and inactivating hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Activated by at least two distinct upstream kinases: the tumor suppressor LKB1 and CaMKK2. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton, probably by indirectly activating myosin. AMPK is a heterotrimer of an alpha catalytic subunit (AMPKA1 or -2), a beta (AMPKB1 or -2) and a gamma non-catalytic subunit (AMPKG1, -2 or -3). Different possible combinations of subunits give rise to 12 different holoenzymes. Binding of ADP or AMP to non-catalytic gamma subunit (PRKAG1, -2 or -3) results in allosteric activation. AMPK is activated by antihyperglycemic drug metformin, a drug prescribed to patients with type 2 diabetes: in vivo, metformin seems to mainly inhibit liver gluconeogenesis. However, metformin can be used to activate AMPK in muscle and other cells in culture or ex vivo. Selectively inhibited by compound C (6-[4-(2-Piperidin-1-yl-ethoxy)-phenyl)]-3-pyridin-4-yl-pyyrazolo[1,5-a] pyrimidine. Activated by resveratrol, a natural polyphenol present in red wine, and S17834, a synthetic polyphenol. Salicylate/aspirin directly activates kinase activity. Studies in the mouse suggest that AMPK2 may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

Protein type: EC 2.7.11.1; Autophagy; Protein kinase, CAMK; Kinase, protein; EC 2.7.11.31; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.27; CAMK group; CAMKL family; AMPK subfamily

Chromosomal Location of Human Ortholog: 1p31

Cellular Component: AMP-activated protein kinase complex; cytoplasm; cytosol; nucleoplasm; nucleus

Molecular Function: AMP-activated protein kinase activity; chromatin binding; histone serine kinase activity; protein binding; protein kinase activity; protein serine/threonine kinase activity; protein serine/threonine/tyrosine kinase activity

Biological Process: carnitine shuttle; cell cycle arrest; cellular response to glucose starvation; cellular response to nutrient levels; fatty acid homeostasis; glucose homeostasis; lipid biosynthetic process; macroautophagy; negative regulation of apoptosis; negative regulation of TOR signaling pathway; positive regulation of autophagy; positive regulation of glycolysis; positive regulation of macroautophagy; protein amino acid phosphorylation; regulation of circadian rhythm; regulation of fatty acid biosynthetic process; regulation of macroautophagy; response to stress; signal transduction
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 Research Articles on PRKAA2    1. These data are in accordance with R6 binding directly to AMPKbeta2 when both proteins detach from the diminishing glycogen particle, which is simultaneous with increased AMPKbeta2 Thr-148 autophosphorylation.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-PRKAA2 antibodyDiseases associated with anti-PRKAA2 antibody
 Products by Pathway  Pathway Diagram
 AMPK Inhibits ChREBP Transcriptional Activation Activity Pathway antibodies  AMPK Inhibits ChREBP Transcriptional Activation Activity Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Activation Of PPARGC1A (PGC-1alpha) By Phosphorylation Pathway antibodies  Activation Of PPARGC1A (PGC-1alpha) By Phosphorylation Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Circadian Rhythm Pathway antibodies  Circadian Rhythm Pathway Diagram
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >14 publications with PRKAA2 and Cardiovascular Diseases
 Insulin Resistance Antibodies  >13 publications with PRKAA2 and Insulin Resistance
 Inflammation Antibodies  >10 publications with PRKAA2 and Inflammation
 Disease Models, Animal Antibodies  >10 publications with PRKAA2 and Disease Models, Animal
 Heart Diseases Antibodies  >9 publications with PRKAA2 and Heart Diseases
 Liver Diseases Antibodies  >6 publications with PRKAA2 and Liver Diseases
 Fibrosis Antibodies  >5 publications with PRKAA2 and Fibrosis
 Cardiomyopathies Antibodies  >5 publications with PRKAA2 and Cardiomyopathies
 Fatty Liver Antibodies  >4 publications with PRKAA2 and Fatty Liver
 Hyperglycemia Antibodies  >4 publications with PRKAA2 and Hyperglycemia
Organs/Tissues associated with anti-PRKAA2 antibody
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >69 publications with PRKAA2 and Muscle
 Liver Antibodies  >23 publications with PRKAA2 and Liver
 Heart Antibodies  >18 publications with PRKAA2 and Heart
 Brain Antibodies  >9 publications with PRKAA2 and Brain
 Lung Antibodies  >8 publications with PRKAA2 and Lung
 Pancreas Antibodies  >7 publications with PRKAA2 and Pancreas
 Ovary Antibodies  >5 publications with PRKAA2 and Ovary
 Embryonic Tissue Antibodies  >3 publications with PRKAA2 and Embryonic Tissue
 Kidney Antibodies  >3 publications with PRKAA2 and Kidney
 Intestine Antibodies  >2 publications with PRKAA2 and Intestine
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