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anti-PYCARD antibody :: Rabbit anti-Human PYCARD Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS9412980
Unit / Price
0.1 mL  /  $255 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

PYCARD, Polyclonal Antibody

★Popular Item★
 Also Known As   

PYCARD Antibody

 Product Synonym Names    PYCARD; ASC; CARD5; MGC10332; TMS
 Product Gene Name   

anti-PYCARD antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    606838
 3D Structure    ModBase 3D Structure for Q9ULZ3
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 Clonality    Polyclonal
 Host    Rabbit
 Species Reactivity    Human
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 Specificity    The antibody detects endogenous level of total PYCARD protein.
 Purity/Purification    Antibodies were purified by affinity purification using immunogen.
 Form/Format    Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
 Concentration    1.0 mg/ml (lot specific)
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 Immunogen Type    Recombinant Protein
 Immunogen Description    Recombinant protein of human PYCARD.
 Target Name    PYCARD
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 Preparation and Storage    Store at -20 degree C
 Other Notes    Small volumes of anti-PYCARD antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-PYCARD antibody

   TMS1 (target of methylation-induced silencing)/ASC (apoptosis-associated speck-like protein containing a CARD), also referred to as PYCARD and CARD5, is a 22-kDa pro-apoptotic protein containing an N-terminal pyrin domain (PYD) and a C-terminal caspase recruitment domain (CARD) (1-2). The TMS1 gene was originally found to be aberrantly methylated and silenced in breast cancer cells (2), and has since been found to be silenced in a number of other cancers, including ovarian cancer (3), glioblastoma (4), melanoma (5), gastric cancer (6), lung cancer (7), and prostate cancer (8). Expression of TMS1 can be induced by pro-apoptotic/inflammatory stimuli (9). During apoptosis TMS1 is re-distributed from the cytosol to the mitochondria and associates with mitochondrial Bax to trigger cytochrome c release and subsequent apoptosis (10). TMS1 has also been found to be a critical component of inflammatory signaling where it associates with and activates caspase-1 in response to pro-inflammatory signals (11).
 Product Categories/Family for anti-PYCARD antibody    Total protein Ab
 Applications Tested/Suitable for anti-PYCARD antibody   

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

 Application Notes for anti-PYCARD antibody    Western blotting: 1:500 - 1:2000
Immunohistochemistry: 1:50 - 1:100
Immunofluorescence: 1:50 - 1:200
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 Western Blot (WB) of anti-PYCARD antibody    Western blot analysis of extracts of various cell lines, using PYCARD antibody.
anti-PYCARD antibody Western Blot (WB) (WB) image
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 Immunohistochemistry (IHC) of anti-PYCARD antibody    Immunohistochemical analysis of paraffin-embedded human kidney using PYCARD antibody at dilution of 1:200 (200x lens).
anti-PYCARD antibody Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of anti-PYCARD antibody    Immunohistochemical analysis of paraffin-embedded rat spleen using PYCARD antibody at dilution of 1:200 (400x lens).
anti-PYCARD antibody Immunohistochemistry (IHC) (IHC) image
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 Immunofluorescence (IF) of anti-PYCARD antibody    Immunofluorescence analysis of HeLa cell using PYCARD antibody. Blue: DAPI for nuclear staining.
anti-PYCARD antibody Immunofluorescence (IF) image
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NCBI/Uniprot data below describe general gene information for PYCARD. It may not necessarily be applicable to this product.
 NCBI GI #    10835256
 NCBI GeneID    29108
 NCBI Accession #    NP_037390.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_013258.4 [Other Products]
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 UniProt Primary Accession #    Q9ULZ3 [Other Products]
 UniProt Secondary Accession #    Q96D12; Q9BSZ5; Q9HBD0; Q9NXJ8 [Other Products]
 UniProt Related Accession #    Q9ULZ3 [Other Products]
 Molecular Weight    15,030 Da
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 NCBI Official Full Name    apoptosis-associated speck-like protein containing a CARD isoform a
 NCBI Official Synonym Full Names    PYD and CARD domain containing
 NCBI Official Symbol    PYCARD [Similar Products]
 NCBI Official Synonym Symbols   
ASC; TMS; TMS1; CARD5; TMS-1
[Similar Products]
 NCBI Protein Information    apoptosis-associated speck-like protein containing a CARD
 UniProt Protein Name    Apoptosis-associated speck-like protein containing a CARD
 UniProt Synonym Protein Names   
Caspase recruitment domain-containing protein 5; PYD and CARD domain-containing protein; Target of methylation-induced silencing 1
 Protein Family    Apoptosis-associated speck-like protein
 UniProt Gene Name    PYCARD [Similar Products]
 UniProt Synonym Gene Names    ASC; CARD5; TMS1; hASC [Similar Products]
 UniProt Entry Name    ASC_HUMAN
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 NCBI Summary for PYCARD    This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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 UniProt Comments for PYCARD    PYCARD: Promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. May be a component of the inflammasome, a protein complex which also includes NALP2, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases. Forms complexes with other DAPIN domain-containing proteins. Interacts with CIAS1/PYPAF1 and PYDC1. Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Detected in the leukemia cell lines HL-60 and U-937, but not in Jurkat T- cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and MOLT-4 lymphocytic leukemia cells. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Tumor suppressor; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: cell soma; mitochondrion; endoplasmic reticulum; cytoplasm; extracellular region; nucleolus; IkappaB kinase complex; nucleus; cytosol

Molecular Function: protein binding; protein homodimerization activity; protease binding; caspase activator activity; Pyrin domain binding

Biological Process: apoptosis; myeloid dendritic cell activation during immune response; positive regulation of apoptosis; positive regulation of caspase activity; regulation of protein stability; positive regulation of JNK cascade; positive regulation of interleukin-1 beta secretion; signal transduction; defense response to Gram-negative bacterium; positive regulation of activated T cell proliferation; activation of NF-kappaB transcription factor; tumor necrosis factor-mediated signaling pathway; positive regulation of phagocytosis; inflammatory response; defense response to virus; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; positive regulation of adaptive immune response; caspase activation; interleukin-1 beta production; positive regulation of interleukin-6 production; negative regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of tumor necrosis factor production; negative regulation of interferon-beta production; myeloid dendritic cell activation; positive regulation of interferon-gamma production; positive regulation of actin filament polymerization; inhibition of NF-kappaB transcription factor; regulation of inflammatory response; innate immune response; regulation of autophagy; positive regulation of transcription factor activity; positive regulation of T cell activation; positive regulation of defense response to virus by host; activation of innate immune response; positive regulation of antigen processing and presentation of peptide antigen via MHC class II
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 Research Articles on PYCARD    1. The proteins (HSP90b, TSM1 and L-plastin) in the current study may hold potential in differentiating between melanoma and benign nevi in diagnostically challenging cases.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-PYCARD antibodyPathways associated with anti-PYCARD antibody
 Reference Product  PubMed Publications
 CASP1 antibody  >238 publications with PYCARD and CASP1
 NLRP3 antibody  >173 publications with PYCARD and NLRP3
 NLRC4 antibody  >31 publications with PYCARD and NLRC4
 AIM2 antibody  >26 publications with PYCARD and AIM2
 NLRP1 antibody  >17 publications with PYCARD and NLRP1
 CASP5 antibody  >8 publications with PYCARD and CASP5
 MEFV antibody  >8 publications with PYCARD and MEFV
 TP53 antibody  >4 publications with PYCARD and TP53
 CARD8 antibody  >4 publications with PYCARD and CARD8
 PSTPIP1 antibody  >2 publications with PYCARD and PSTPIP1
 Products by Pathway  Pathway Diagram
 C-type Lectin Receptors (CLRs) Pathway antibodies  C-type Lectin Receptors (CLRs) Pathway Diagram
 CLEC7A (Dectin-1) Signaling Pathway antibodies  CLEC7A (Dectin-1) Signaling Pathway Diagram
 CLEC7A/inflammasome Pathway antibodies  CLEC7A/inflammasome Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Cytosolic DNA-sensing Pathway antibodies  Cytosolic DNA-sensing Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Inflammasomes Pathway antibodies  Inflammasomes Pathway Diagram
 Influenza A Pathway antibodies  Influenza A Pathway Diagram
 Influenza A Pathway antibodies  Influenza A Pathway Diagram
Diseases associated with anti-PYCARD antibodyOrgans/Tissues associated with anti-PYCARD antibody
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >157 publications with PYCARD and Inflammation
 Necrosis Antibodies  >57 publications with PYCARD and Necrosis
 Disease Models, Animal Antibodies  >33 publications with PYCARD and Disease Models, Animal
 Nervous System Diseases Antibodies  >22 publications with PYCARD and Nervous System Diseases
 Brain Diseases Antibodies  >19 publications with PYCARD and Brain Diseases
 Adenocarcinoma Antibodies  >14 publications with PYCARD and Adenocarcinoma
 Kidney Diseases Antibodies  >13 publications with PYCARD and Kidney Diseases
 Liver Diseases Antibodies  >10 publications with PYCARD and Liver Diseases
 Colorectal Neoplasms Antibodies  >8 publications with PYCARD and Colorectal Neoplasms
 Fibrosis Antibodies  >8 publications with PYCARD and Fibrosis
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >92 publications with PYCARD and Blood
 Bone Antibodies  >44 publications with PYCARD and Bone
 Brain Antibodies  >29 publications with PYCARD and Brain
 Kidney Antibodies  >25 publications with PYCARD and Kidney
 Lung Antibodies  >24 publications with PYCARD and Lung
 Liver Antibodies  >20 publications with PYCARD and Liver
 Vascular Antibodies  >13 publications with PYCARD and Vascular
 Intestine Antibodies  >13 publications with PYCARD and Intestine
 Skin Antibodies  >13 publications with PYCARD and Skin
 Connective Tissue Antibodies  >5 publications with PYCARD and Connective Tissue
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