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anti-Pxn antibody :: Rabbit Paxillin (Ser-178) Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS472047
Testing Data
Unit / Price
0.1 mL  /  $380 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Paxillin (Ser-178) (Pxn), Polyclonal Antibody

★Popular Item★
 Also Known As   

Anti-Paxillin (Ser-178), phospho-specific

 Product Synonym Names    Paxillin (Ser-178); phospho-specific; Paxillin
 Product Gene Name   

anti-Pxn antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 12; NC_005111.2 (42322193..42370507, complement). Location: 12q16
 3D Structure    ModBase 3D Structure for Q66H76
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 Clonality    Polyclonal
 Host    Rabbit
 Species Reactivity    Human, Rat, Mouse
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 Specificity    This antibody detects a 68kDa* protein corresponding to the molecular mass of phosphorylated paxillin on SDS-PAGE immunoblots of EGF treated A431 cells, but not in A431 control cells. Similar results were seen in calyculin A treated human A431 and aortic endothelial cells.
 Purity/Purification    Affinity Purified
 Form/Format    Rabbit polyclonal, affinity-purified antibody is supplied in 100 ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
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 Immunogen    Phospho-Paxillin (Ser-178) synthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 178 of human paxillin. This human sequence is highly conserved in rat and mouse paxillin.
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 Preparation and Storage    Store at -20 degree C. Do not aliquot. Stable for 1 year.
 Other Notes    Small volumes of anti-Pxn antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-Pxn antibody

   Paxillin, a focal adhesion protein, is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and SH3-/SH2-binding domains that participate in a variety of protein-protein interactions with kinases, GTPase-activating proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at both tyrosine and serine sites. Serine phosphorylation of paxillin occurs in response to growth-factor activation and fibronectins. Both JNK1 and cdc2 kinases can phosphorylate serine 178 in paxillin. The mutant form of paxillin (S178A) decreases the migration of keratocytes and epithelial cells. Thus, phosphorylation paxillin at serine 178 may be important during cell migration.
 Applications Tested/Suitable for anti-Pxn antibody   

ELISA, Western Blot

 Application Notes for anti-Pxn antibody    ELISA: 1:2000
Western Blot: 1:500
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 Testing Data of anti-Pxn antibody    anti-Pxn antibody Testing Data image
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NCBI/Uniprot data below describe general gene information for Pxn. It may not necessarily be applicable to this product.
 NCBI GI #    58865866
 NCBI GeneID    360820
 NCBI Accession #    NP_001012147.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001012147.1 [Other Products]
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 UniProt Primary Accession #    Q66H76 [Other Products]
 UniProt Related Accession #    Q1EG89; Q66H76 [Other Products]
 Molecular Weight    63888 Da [Similar Products]
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 NCBI Official Full Name    paxillin
 NCBI Official Synonym Full Names    paxillin
 NCBI Official Symbol    Pxn [Similar Products]
 NCBI Official Synonym Symbols   
Pxn
[Similar Products]
 NCBI Protein Information    paxillin; myocardial ischemic preconditioning associated protein 7
 UniProt Protein Name    Paxillin
 Protein Family    Paxillin
 UniProt Gene Name    Pxn [Similar Products]
 UniProt Entry Name    PAXI_RAT
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 UniProt Comments for Pxn    Function: Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion)

Subunit structure: Binds to vinculin, to the SH3 domain of c-SRC and to focal adhesion kinase. Interacts with GIT1 and NUDT16L1/SDOS. Component of cytoplasmic complexes, which also contain GIT1, ARHGEF6 and PAK1. Binds ASAP2 and PDCD10

By similarity. Interacts with PARVA and TGFB1I1. Ref.2 Ref.3

Subcellular location: Cytoplasm › cytoskeleton

By similarity. Cell junction › focal adhesion

Post-translational modification: Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens

By similarity. Ref.4

Sequence similarities: Belongs to the paxillin family.Contains 4 LIM zinc-binding domains.
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Product References and Citations for anti-Pxn antibody

   • Huang, C. et al. (2003) Nature 424:219-223.
• Huang, C. et al. (2004) Cell Cycle 3(1):4-6.
• Woodrow, M.A. (2003) Exp. Cell. Res. 287(2):325-338.
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 Research Articles on Pxn    1. Talin and paxillin showed a major distributional change between different hormone regimes, estrogen and progesterone
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-Pxn antibodyPathways associated with anti-Pxn antibody
 Reference Product  PubMed Publications
 Ilk antibody  >8 publications with Pxn and Ilk
 Products by Pathway  Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 EGFR1 Signaling Pathway antibodies  EGFR1 Signaling Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Gab1 Signalosome Pathway antibodies  Gab1 Signalosome Pathway Diagram
 IL-3 Signaling Pathway antibodies  IL-3 Signaling Pathway Diagram
Diseases associated with anti-Pxn antibodyOrgans/Tissues associated with anti-Pxn antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >171 publications with Pxn and Neoplasms
 Neoplasm Metastasis Antibodies  >32 publications with Pxn and Neoplasm Metastasis
 Breast Neoplasms Antibodies  >29 publications with Pxn and Breast Neoplasms
 Inflammation Antibodies  >23 publications with Pxn and Inflammation
 Cell Transformation, Neoplastic Antibodies  >21 publications with Pxn and Cell Transformation, Neoplastic
 Adenocarcinoma Antibodies  >19 publications with Pxn and Adenocarcinoma
 Leukemia Antibodies  >16 publications with Pxn and Leukemia
 Lung Diseases Antibodies  >16 publications with Pxn and Lung Diseases
 Neoplasms, Experimental Antibodies  >16 publications with Pxn and Neoplasms, Experimental
 Necrosis Antibodies  >14 publications with Pxn and Necrosis
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >65 publications with Pxn and Muscle
 Lung Antibodies  >36 publications with Pxn and Lung
 Kidney Antibodies  >36 publications with Pxn and Kidney
 Embryonic Tissue Antibodies  >29 publications with Pxn and Embryonic Tissue
 Brain Antibodies  >26 publications with Pxn and Brain
 Heart Antibodies  >19 publications with Pxn and Heart
 Colon Antibodies  >18 publications with Pxn and Colon
 Eye Antibodies  >17 publications with Pxn and Eye
 Prostate Antibodies  >12 publications with Pxn and Prostate
 Placenta Antibodies  >7 publications with Pxn and Placenta
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