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anti-PRLR antibody :: Rabbit Prolactin Receptor Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS176232
Unit / Price
0.1 mg  /  $245 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Prolactin Receptor (PRLR), Polyclonal Antibody

★Popular Item★
 Also Known As   

Anti-Prolactin Receptor antibody

 Product Synonym Names    Prolactin receptor; prolactin receptor; AI987712 antibody; CLONE SPM213 antibody; CPRLP antibody; Delta 4-delta 7/11 truncated prolactin receptor antibody; Delta 4-SF1b truncated prolactin receptor antibody; hPRL receptor antibody; hPRLrI antibody; Lactogen receptor antibody; MGC105486 antibody; OPR antibody; OTTHUMP00000115998 antibody; Pr-1 antibody; Pr-3 antibody; PRL R antibody; PRL-R antibody; PRLR antibody; Prlr-rs1 antibody; PRLR_HUMAN antibody; Prolactin receptor a antibody; Prolactin receptor antibody; Prolactin receptor delta 7/11 antibody; RATPRLR antibody; Secreted prolactin binding protein antibody; Truncated testis-specific box 1-C prolactin receptor antibody; wu:fj65c07 antibody
 Product Gene Name   

anti-PRLR antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 3D Structure    ModBase 3D Structure for Q08501
 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Reacts with: Human, Mouse
Predicted to work with: Rat
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 Specificity    No cross reactivity with other proteins
 Purity/Purification    Immunogen affinity purified.
 Form/Format    Lyophilized
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 Immunogen    A synthetic peptide corresponding to a sequence at the C-terminus of mouse Prolactin Receptor (591-605aa QLGRLDYLDPTCFMH), identical to the related rat sequence, and different from the related human sequence by three amino acids.
 Gene Name    Prolactin receptor
 Contents    Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
 Reconstitution    Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
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 Preparation and Storage    At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of anti-PRLR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-PRLR antibody

   Description: Rabbit IgG polyclonal antibody for Prolactin receptor(PRLR) detection. Tested with WB, IHC-P in Human, Mouse, Rat.
Background: PRLR(Prolactin Receptor), is a cytokine receptor. By somatic cell hybrid analysis and by in situ hybridization, Arden et al.(1989, 1990) demonstrated that the prolactin receptor gene resides in the same chromosomal region as the growth hormone receptor gene, which has been mapped to 5p13-p12. Cunningham et al.(1990) demonstrated that zinc greatly increases the affinity of GH for the extracellular binding domain of PRLR, although it is not required for binding of GH to the growth hormone receptor or for binding of prolactin to the prolactin receptor. By mutational analysis, they showed that a cluster of 3 residues(histidine-18, histidine-21, and glutamic acid-174) in GH and histidine-188 in PRLR(conserved in all PRL receptors but not GH receptors) are likely zinc-ion ligands.
 Applications Tested/Suitable for anti-PRLR antibody   

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

 Application Notes for anti-PRLR antibody    Western Blot:
Concentration: 0.1-0.5ug/ml
Tested Species: Hu
Predicted Species: Ms, Rat

Immunohistochemistry (Paraffin-embedded Section):
Concentration: 0.5-1ug/ml
Tested Species: Hu, Rat
Predicted Species: Ms
Antigen Retrieval: By Heat

Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
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 Western Blot (WB) of anti-PRLR antibody    Figure 1. Western blot analysis of PRLR using anti- PRLR antibody (MBS176232).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat PC-12 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- PRLR antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PRLR at approximately 90KD. The expected band size for PRLR is at 70KD.
anti-PRLR antibody Western Blot (WB) (WB) image
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 Immunohistochemistry (IHC) of anti-PRLR antibody    Anti-PRLR antibody, MBS176232, IHC(P)
IHC(P): Human Mammary Cancer Tissue
anti-PRLR antibody Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of anti-PRLR antibody    Anti-PRLR antibody, MBS176232, IHC(P)
IHC(P): Rat Testis Tissue
anti-PRLR antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for PRLR. It may not necessarily be applicable to this product.
 NCBI GI #    730343
 NCBI GeneID    19116
 NCBI Accession #    Q08501.1 [Other Products]
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 UniProt Primary Accession #    Q08501 [Other Products]
 UniProt Secondary Accession #    P15212; P15213; Q62099 [Other Products]
 UniProt Related Accession #    Q08501 [Other Products]
 Molecular Weight    33,633 Da
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 NCBI Official Full Name    Prolactin receptor
 NCBI Official Synonym Full Names    prolactin receptor
 NCBI Official Symbol    Prlr [Similar Products]
 NCBI Official Synonym Symbols   
Pr-1; Pr-3; AI987712; Prlr-rs1
[Similar Products]
 NCBI Protein Information    prolactin receptor; PRL-R; prolactin receptor related sequence 1
 UniProt Protein Name    Prolactin receptor
 Protein Family    Prolactin receptor
 UniProt Gene Name    Prlr [Similar Products]
 UniProt Synonym Gene Names    PRL-R [Similar Products]
 UniProt Entry Name    PRLR_MOUSE
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 UniProt Comments for PRLR    PRLR: receptor for the anterior pituitary hormone prolactin and placental lactogen I and II. Interacts with Cyclophilin A, differentially regulating various signaling pathways from the PrlR. Prolactin signaling is attenuated by threonine and tyrosine phosphorylation. Two alternative splice isoforms have been identified.

Protein type: Receptor, cytokine; Membrane protein, integral

Cellular Component: cell surface; membrane; integral to membrane; intracellular

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; metal ion binding; peptide hormone binding; protein kinase binding; prolactin receptor activity

Biological Process: lactation; regulation of cell adhesion; transmembrane receptor protein tyrosine kinase activation (dimerization); regulation of epithelial cell differentiation; JAK-STAT cascade
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 Research Articles on PRLR    1. This study showed in knockout mice showed no effect of PRL and PRL-R gene ablation on heat and cold hyperalgesia in male mice, while heat hyperlgesia were reduced 3-72 h post-surgery in female PRL and PRL-R knockout mice
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-PRLR antibodyDiseases associated with anti-PRLR antibody
 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Growth Hormone Receptor Signaling Pathway antibodies  Growth Hormone Receptor Signaling Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Neuroactive Ligand-receptor Interaction Pathway antibodies  Neuroactive Ligand-receptor Interaction Pathway Diagram
 Neuroactive Ligand-receptor Interaction Pathway antibodies  Neuroactive Ligand-receptor Interaction Pathway Diagram
 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >82 publications with PRLR and Breast Neoplasms
 Carcinoma Antibodies  >42 publications with PRLR and Carcinoma
 Mammary Neoplasms, Animal Antibodies  >18 publications with PRLR and Mammary Neoplasms, Animal
 Neoplasms, Experimental Antibodies  >12 publications with PRLR and Neoplasms, Experimental
 Infertility, Female Antibodies  >11 publications with PRLR and Infertility, Female
 Liver Diseases Antibodies  >10 publications with PRLR and Liver Diseases
 Necrosis Antibodies  >8 publications with PRLR and Necrosis
 Mammary Neoplasms, Experimental Antibodies  >8 publications with PRLR and Mammary Neoplasms, Experimental
 Weight Gain Antibodies  >8 publications with PRLR and Weight Gain
 Inflammation Antibodies  >7 publications with PRLR and Inflammation
Organs/Tissues associated with anti-PRLR antibody
 Organ/Tissue Name  Pubmed Publications
 Mammary Gland Antibodies  >82 publications with PRLR and Mammary Gland
 Liver Antibodies  >58 publications with PRLR and Liver
 Brain Antibodies  >55 publications with PRLR and Brain
 Ovary Antibodies  >53 publications with PRLR and Ovary
 Kidney Antibodies  >43 publications with PRLR and Kidney
 Pituitary Gland Antibodies  >43 publications with PRLR and Pituitary Gland
 Embryonic Tissue Antibodies  >28 publications with PRLR and Embryonic Tissue
 Adipose Tissue Antibodies  >27 publications with PRLR and Adipose Tissue
 Connective Tissue Antibodies  >25 publications with PRLR and Connective Tissue
 Prostate Antibodies  >21 publications with PRLR and Prostate
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