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anti-RBBP4 antibody :: Rabbit RBBP4 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS840864
Testing Data
Unit / Price
0.1 mg  /  $385 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

RBBP4, Polyclonal Antibody

 Also Known As   

RBBP4 Antibody

 Product Synonym Names    NURF55; RBAP48
 Product Gene Name   

anti-RBBP4 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    602923
 3D Structure    ModBase 3D Structure for Q09028
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human, Mouse, Rat
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 Purity/Purification    Affinity purified
 Form/Format    100 ug of antibody in 100 ul PBS containing 0.02% sodium azide, 50% glycerol, pH 7.3
Appearance: Colorless liquid
 Concentration    1 mg/ml (lot specific)
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 Immunogen    Recombinant protein of human RBBP4
 Positive Control    Western Blot: Various cell lysates
 Handling    The antibody solution should be gently mixed before use.
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 Preparation and Storage    At -20 degree C
Shelf Life: 12 months
 Other Notes    Small volumes of anti-RBBP4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-RBBP4 antibody

   Polyclonal antibody to detect RBBP4 in human, mouse and rat samples.

Background: Retinoblastoma associated proteins 46 and 48 (RBAP46 and RBAP48? also known as RBBP7 and RBBP4) were first characterized in human cells as proteins that bind to the retinoblastoma (Rb) tumor suppressor protein. Since then, these proteins have been shown to be components of many protein  complexes involved in  chromatin  regulation, including the chromatin  assembly factor 1 (CAF-1) complex and type B histone acetyltransferase complex HAT1, both of which function in chromatin  assembly during DNA  replication. RBAP46 and RBAP48 are also found in the nucleosome remodeling factor complex NURF, the nucleosome remodeling and histone de-acetylation complex NuRD, and the Sin3/HDAC histone de-acetylation complex. More recently, RBAP46 and RBAP48 were identified as components of the polycomb repressor complex PRC2, which also contains EED and Ezh2. RBAP46 and RBAP48 bind to the histone fold region of histone H4 and are believed to target these chromatin remodeling, histone acetylation, and histone de-acetylation complexes to their histone substrates.
 Product Categories/Family for anti-RBBP4 antibody    Epigenetics; Writer Enzymes; Histone Acetyltransferases (HATs); Antibodies & Blocking PeptidesAntibodies & Supporting Tools; Primary Antibodies (A-Z)
 Applications Tested/Suitable for anti-RBBP4 antibody   

Western Blot (WB), Immunohistochemistry (IHC)

 Application Notes for anti-RBBP4 antibody    Western blot: 1:500 - 1:2000, IHC: 1:50 - 1:200.
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 Testing Data of anti-RBBP4 antibody    anti-RBBP4 antibody Testing Data image
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NCBI/Uniprot data below describe general gene information for RBBP4. It may not necessarily be applicable to this product.
 NCBI GI #    207029415
 NCBI GeneID    5928
 NCBI Accession #    NP_001128727.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001135255.1 [Other Products]
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 UniProt Primary Accession #    Q09028 [Other Products]
 UniProt Secondary Accession #    P31149; Q53H02; Q96BV9; B2R6G9; B4DRH0; D3DPQ3 [Other Products]
 UniProt Related Accession #    Q09028 [Other Products]
 Molecular Weight    43,482 Da
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 NCBI Official Full Name    histone-binding protein RBBP4 isoform b
 NCBI Official Synonym Full Names    retinoblastoma binding protein 4
 NCBI Official Symbol    RBBP4 [Similar Products]
 NCBI Official Synonym Symbols   
NURF55; RBAP48; lin-53
[Similar Products]
 NCBI Protein Information    histone-binding protein RBBP4
 UniProt Protein Name    Histone-binding protein RBBP4
 UniProt Synonym Protein Names   
Chromatin assembly factor 1 subunit C; CAF-1 subunit C; Chromatin assembly factor I p48 subunit; CAF-I 48 kDa subunit; CAF-I p48; Nucleosome-remodeling factor subunit RBAP48; Retinoblastoma-binding protein 4; RBBP-4; Retinoblastoma-binding protein p48
 Protein Family    Histone-binding protein
 UniProt Gene Name    RBBP4 [Similar Products]
 UniProt Synonym Gene Names    RBAP48; CAF-1 subunit C; CAF-I 48 kDa subunit; CAF-I p48; RBBP-4 [Similar Products]
 UniProt Entry Name    RBBP4_HUMAN
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 NCBI Summary for RBBP4    This gene encodes a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. It is present in protein complexes involved in histone acetylation and chromatin assembly. It is part of the Mi-2 complex which has been implicated in chromatin remodeling and transcriptional repression associated with histone deacetylation. This encoded protein is also part of co-repressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation. This protein also seems to be involved in transcriptional repression of E2F-responsive genes. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]
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 UniProt Comments for RBBP4    RBBP4: Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex. Interacts with SUV39H1 and HDAC7. Binds directly to helix 1 of the histone fold of histone H4, a region that is not accessible when H4 is in chromatin. Subunit of the chromatin assembly factor 1 (CAF-1) complex, which is composed of RBBP4, CHAF1B and CHAF1A. Subunit of the core histone deacetylase (HDAC) complex, which is composed of HDAC1, HDAC2, RBBP4 and RBBP7. The core HDAC complex associates with SIN3A, ARID4B/SAP180, SAP18, SAP30, SAP130, SDS3/SAP45 and possibly ARID4A/RBP1 and ING1 to form the SIN3 HDAC complex. The core HDAC complex may also associate with MTA2, MBD3, CHD3 and CHD4 to form the nucleosome remodeling and histone deacetylase complex (the NuRD complex). The NuRD complex may also interact with MBD3L1 and MBD3L2. Interacts with MTA1. Subunit of the PRC2/EED-EZH2 complex, which is composed of at least EED, EZH2, RBBP4, RBBP7 and SUZ12. The PRC2/EED-EZH2 complex may also associate with HDAC1. Component of the PRC2/EED- EZH1 complex, which includes EED, EZH1, SUZ12, RBBP4 and AEBP2. Part of the nucleosome remodeling factor (NURF) complex which consists of SMARCA1; BPTF; RBBP4 and RBBP7. Interacts with the viral protein-binding domain of the retinoblastoma protein (RB1). Interacts with SPEN/MINT. Interacts with BRCA1. Interacts with CREBBP, and this interaction may be enhanced by the binding of phosphorylated CREB1 to CREBBP. Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2. Belongs to the WD repeat RBAP46/RBAP48/MSI1 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 1p35.1

Cellular Component: nucleoplasm; protein complex; Sin3 complex; ESC/E(Z) complex; nuclear chromatin; NuRD complex; nucleus

Molecular Function: DNA-dependent ATPase activity; protein binding; nucleosomal DNA binding; histone binding; histone deacetylase binding

Biological Process: establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; regulation of cell cycle; chromatin assembly; DNA replication-dependent nucleosome assembly; regulation of gene expression, epigenetic; chromatin remodeling; nucleosome assembly; negative regulation of cell proliferation; DNA replication-independent nucleosome assembly at centromere; DNA replication-independent nucleosome assembly; regulation of transcription, DNA-dependent; negative regulation of gene expression, epigenetic; ATP-dependent chromatin remodeling; gene expression; mitotic cell cycle; G2/M transition of mitotic cell cycle; DNA replication
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 Research Articles on RBBP4    1. Our RBBP4-PHF6 complex structure provides insights into the molecular basis of PHF6-NuRD complex interaction and implicates a role for PHF6 in chromatin structure modulation and gene regulation.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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