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anti-RpII215 antibody :: Rabbit RNA Polymerase II, Rpb1 subunit CTD Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS614614 anti-RpII215 antibody
Unit / Price
0.1 mL  /  $525 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

RNA Polymerase II, Rpb1 subunit CTD (RpII215), Polyclonal Antibody

 Also Known As   

RNA Polymerase II, Rpb1 subunit, phosphorylated CTD (Ser2/5) (Rpb1, Pol2)

 Product Synonym Names    Anti -RNA Polymerase II, Rpb1 subunit, phosphorylated CTD (Ser2/5) (Rpb1, Pol2)
 Product Gene Name   

anti-RpII215 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: X; NC_004354.3 (11456806..11464355, complement). Location: 10C6-10C6; 1-35.66 cM
 3D Structure    ModBase 3D Structure for P04052
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 Clonality    Polyclonal
 Host    Rabbit
 Species Reactivity    Human, Mouse, Rat
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 Specificity    Detects endogenous levels of Rpb1 only when the carboxy-termial domain heptapeptide repeat [Tyr1, Ser2, Pro3, Thr4, Ser5, Pro6, Ser7] is phosphorylated at serine 2/5. Species Crossreactivity
 Purity/Purification    Affinity Purified
Puriifed by Protein A and peptide affinity chromatography.
 Form/Format    Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.
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 Immunogen    Synthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding Ser2/5 of the Rpb1 CTD heptapeptide.
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 Preparation and Storage    May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
 Other Notes    Small volumes of anti-RpII215 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-RpII215 antibody

   RNA polymerase II is a complex of 12 proteins that participates in transcription, mRNA processing, and transcription-coupled DNA repair (1,2). The largest subunit, Rpb1, contains a unique heptapeptide: Tyr1, Ser2, Pro3, Thr4, Ser5, Pro6, Ser7, which is repeated up to 52 times in the carboxy-terminal domain CTD) of Rpb1 (3). This CTD is phosphorylated by cyclin-dependent kinases (cdks), p44/42 MAPK, DNAPKcs, and c-Abl (4). DNA damage caused by UV-light, hydrogen peroxide, or cisplatin results in ubiquitination and proteasomal degradation of Rpb1 (1,3). The kinase inhibitor 5, 6-dichloro-1-b- D-ribofuranosylbenzimidazole prevents ubiquitination of Rpb1, suggesting that CTD phosphorylation is required for proteolysis (5).
 Product Categories/Family for anti-RpII215 antibody    Antibodies; Abs to Enzymes, Polymerase
 Applications Tested/Suitable for anti-RpII215 antibody   

Western Blot (WB), Immunohistochemistry (IHC)

 Application Notes for anti-RpII215 antibody    Suitable for use in Western Blot and Immunohistochemistry.
Dilution: Western Blot: 1:1000
Immunohistochemistry (Paraffin): 1:25 (Citrate/TBST)
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NCBI/Uniprot data below describe general gene information for RpII215. It may not necessarily be applicable to this product.
 NCBI GI #    17530899
 NCBI GeneID    32100
 NCBI Accession #    NP_511124.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_078569.2 [Other Products]
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 UniProt Primary Accession #    P04052 [Other Products]
 UniProt Secondary Accession #    Q9VYX6 [Other Products]
 UniProt Related Accession #    P04052 [Other Products]
 Molecular Weight    209,168 Da [Similar Products]
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 NCBI Official Full Name    RNA polymerase II 215kD subunit
 NCBI Official Synonym Full Names    RNA polymerase II 215kD subunit
 NCBI Official Symbol    RpII215 [Similar Products]
 NCBI Official Synonym Symbols   
5; 8WG16; CG1554; CTD; DmelCG1554; dRpb1; dRPB1; H5; II; l(1)10Ca; l(1)DC912; l(1)DF912; l(1)G0040; l(1)L5; L5; POL; pol II; Pol II; Pol II CTD; Pol II Ser5P; Pol II0[ser2]; Pol II0[ser5]; Pol IIa; Pol IIo; Pol IIo[ser2]; Pol IIo[Ser2]; Pol IIo[ser5]; Pol
[Similar Products]
 NCBI Protein Information    CG1554 gene product from transcript CG1554-RA; CG1554-PA; Pol II CTD; Pol II large subunit; RNA Pol II; RNA polymerase II; RNA polymerase II 215kD subunit; RNA polymerase II large subunit; RNA polymerase IIo; RNA polymeraseII-215 kd subunit; RNA-polymerase II; RpII215-PA; Ultrabithorax-like; polymerase II CTD
 UniProt Protein Name    DNA-directed RNA polymerase II subunit RPB1
 UniProt Synonym Protein Names   
DNA-directed RNA polymerase III largest subunit
 UniProt Gene Name    RpII215 [Similar Products]
 UniProt Synonym Gene Names    RNA polymerase II subunit B1 [Similar Products]
 UniProt Entry Name    RPB1_DROME
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 UniProt Comments for RpII215    Function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing

By similarity.

Catalytic activity: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Subunit structure: Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits

By similarity.

Subcellular location: Nucleus

By similarity.

Post-translational modification: The tandem 7 residues repeats in the C-terminal domain (CTD) can be highly phosphorylated. The phosphorylation activates Pol II. Phosphorylation occurs mainly at residues 'Ser-2' and 'Ser-5' of the heptapepdtide repeat. The phosphorylation state is believed to result from the balanced action of site-specific CTD kinases and phosphatase, and a 'CTD code' that specifies the position of Pol II within the transcription cycle has been proposed.

Miscellaneous: The binding of ribonucleoside triphosphate to the RNA polymerase II transcribing complex probably involves a two-step mechanism. The initial binding seems to occur at the entry (E) site and involves a magnesium ion temporarily coordinated by three conserved aspartate residues of the two largest RNA Pol II subunits. The ribonucleoside triphosphate is transferred by a rotation to the nucleotide addition (A) site for pairing with the template DNA. The catalytic A site involves three conserved aspartate residues of the RNA Pol II largest subunit which permanently coordinate a second magnesium ion.

Sequence similarities: Belongs to the RNA polymerase beta' chain family.
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 Research Articles on RpII215    1. A novel single-nucleotide mutation in the 3' UTR of the RNPII215 gene leads to a reduced number of nuclear divisions that is accompanied by premature transcription of early zygotic genes and cellularization.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-RpII215 antibodyPathways associated with anti-RpII215 antibody
 Reference Product  PubMed Publications
 RpII140 antibody  >3 publications with RpII215 and RpII140
 RpII18 antibody  >1 publications with RpII215 and RpII18
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 Formation Of Transcription-coupled NER (TC-NER) Repair Complex Pathway antibodies  Formation Of Transcription-coupled NER (TC-NER) Repair Complex Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 MicroRNA (miRNA) Biogenesis Pathway antibodies  MicroRNA (miRNA) Biogenesis Pathway Diagram
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 Processing Of Capped Intron-Containing Pre-mRNA Pathway antibodies  Processing Of Capped Intron-Containing Pre-mRNA Pathway Diagram
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