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anti-PCGF3 antibody :: Goat RNF3 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS420175
Western Blot (WB)
Unit / Price
0.1 mg  /  $300 +1 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

RNF3 (PCGF3), Polyclonal Antibody

 Also Known As   

Goat anti-RNF3 (247 aa isoform) Antibody

 Product Synonym Names    RNF3; DONG1; RNF3A; MGC40413; ring finger protein 3; PCGF3; polycomb group ring finger 3; DKFZp686D20235; FLJ36550; FLJ43813; MGC129615; RNF3 antibody; DONG1 antibody; RNF3A antibody; MGC40413 antibody; ring finger protein 3 antibody; PCGF3 antibody; polycomb group ring finger 3 antibody; DKFZp686D20235 antibody; FLJ36550 antibody; FLJ43813 antibody; MGC129615 antibody; RNF3 (247 aa isoform)
 Product Gene Name   

anti-PCGF3 antibody

[Similar Products]
 Product Synonym Gene Name    RNF3 [Similar Products]
 Antibody/Peptide Pairs    RNF3 peptide (MBS426072) is used for blocking the activity of RNF3 antibody (MBS420175)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence    PLLLHYRPKM DLL
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 Clonality    Polyclonal
 Host    Goat
 Species Reactivity    Tested: Human; Expected from sequence similarity: Human, Mouse, Dog, Cow
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 Purity/Purification    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
 Form/Format    Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
 Concentration    100ug specific antibody in 200ul (lot specific)
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 Immunogen    Peptide with sequence C-PLLLHYRPKMDLL, from the C Terminus of the protein sequence according to NP_006306.2.
 Epitope    C Terminus
 Note    This antibody is expected to recognise the 247 aa isoform only (NP_006306), not the 132 aa isoform (NP_694982).
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 Preparation and Storage    Aliquot and store at -20 degree C. Minimize freezing and thawing.
 ISO Certification    Manufactured in an ISO 9001:2015 Certified Laboratory.
 Other Notes    Small volumes of anti-PCGF3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-PCGF3 antibody   

Peptide ELISA (EIA), Western Blot (WB)

 Application Notes for anti-PCGF3 antibody    Peptide ELISA: Antibody detection limit dilution 1: 2000.
Western Blot: Approx 35kDa band seen in Human Heart lysate [Predicted MW of approx.27kDa according to NP_006306]. Recommended for use at 0.5-2ug/ml.
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 Western Blot (WB) of anti-PCGF3 antibody    Staining (1ug/ml) of Human Heart lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
anti-PCGF3 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for PCGF3. It may not necessarily be applicable to this product.
 NCBI GI #    31742478
 NCBI GeneID    10336
 NCBI Accession #    NP_006306.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_006315.5 [Other Products]
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 UniProt Secondary Accession #    O15262; D3DVN1 [Other Products]
 UniProt Related Accession #    Q3KNV8 [Other Products]
 Molecular Weight    28,748 Da
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 NCBI Official Full Name    polycomb group RING finger protein 3
 NCBI Official Synonym Full Names    polycomb group ring finger 3
 NCBI Official Symbol    PCGF3 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    polycomb group RING finger protein 3
 UniProt Protein Name    Polycomb group RING finger protein 3
 UniProt Synonym Protein Names   
RING finger protein 3A
 Protein Family    Polycomb group RING finger protein
 UniProt Gene Name    PCGF3 [Similar Products]
 UniProt Synonym Gene Names    RNF3; RNF3A [Similar Products]
 UniProt Entry Name    PCGF3_HUMAN
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 NCBI Summary for PCGF3    The protein encoded by this gene contains a C3HC4 type RING finger, which is a motif known to be involved in protein-protein interactions. The specific function of this protein has not yet been determined. [provided by RefSeq, Jul 2008]
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 UniProt Comments for PCGF3    PCGF3: Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 4p16.3

Cellular Component: nucleus; PcG protein complex

Molecular Function: protein binding; zinc ion binding

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-PCGF3 antibody
 Products by Pathway  Pathway Diagram
 Signaling Pathways Regulating Pluripotency Of Stem Cells antibodies  Signaling Pathways Regulating Pluripotency Of Stem Cells Diagram
 Signaling Pathways Regulating Pluripotency Of Stem Cells antibodies  Signaling Pathways Regulating Pluripotency Of Stem Cells Diagram
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