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anti-RNF34 antibody :: Rabbit anti-Human RNF34 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS240834
Unit / Price
0.05 mg  /  $530 +1 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

RNF34, Polyclonal Antibody

 Also Known As   

Rabbit Polyclonal to Human RNF34

 Product Synonym Names    Anti-RNF34 Antibody (aa1-373) IHC-plus; RNF34; CARP1; Caspase regulator CARP1; CARP-1; FYVE-RING finger protein Momo; HRFI; RFI; RIF; RIFF; RING finger protein 34; RING finger protein RIFF; Human RNF34
 Product Gene Name   

anti-RNF34 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    608299
 3D Structure    ModBase 3D Structure for Q969K3
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 Clonality    Polyclonal
 Host    Rabbit
 Species Reactivity    Human
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 Specificity    Amino acids 1-373 of human RNF34 protein.
 Purity/Purification    Protein A Purified
 Form/Format    Lyophilized.
 Concentration    1 mg/ml (lot specific)
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 Target Species    Human
 Immunogen Description    Recombinant human RNF34.
 Immunogen Type    Recombinant protein
 Immunogen    RNF34 antibody was raised against recombinant human RNF34.
 Antigen Modification    aa1-373
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 Preparation and Storage    +4 degree C or -20 degree C, Avoid repeated freezing and thawing.
 Other Notes    Small volumes of anti-RNF34 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-RNF34 antibody   

Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)

 Application Notes for anti-RNF34 antibody    ELISA (1:4000 - 1:20000), IHC-P (10 ug/ml), WB (1:500 - 1:3000)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...
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 Immunohistochemistry (IHC) of anti-RNF34 antibody    Anti-RNF34 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
anti-RNF34 antibody Immunohistochemistry (IHC) (IHC) image
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 Western Blot (WB) of anti-RNF34 antibody    Anti-RNF34 Antibody - Western Blot. Western blot of Protein A Purified anti-RNF34 antibody shows detection of human RNF34 (arrowhead) in lysate. Lanes corresponding to empty vector 293T cell lysate (mock, left); RNF34 transfected lysate (middle) and RFFL transfected lysate (right), are shown using 20 ul of lysate per lane. Lysates were prepared from equivalent numbers of cells. Data presented demonstrate that this reagent is specific for RNF34. After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:1000 using 5% BLOTTO, 0.1% Tween-20 in PBS as the diluent. Incubation occurred for 1 h at room temperature. Personal Communication, Srinivasa Srinivasula, CCR-NCI, Bethesda, MD.
anti-RNF34 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for RNF34. It may not necessarily be applicable to this product.
 NCBI GI #    37595537
 NCBI GeneID    80196
 NCBI Accession #    NP_079402.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_025126.3 [Other Products]
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 UniProt Primary Accession #    Q969K3 [Other Products]
 UniProt Secondary Accession #    Q8NG47; Q9H6W8; B7Z933 [Other Products]
 UniProt Related Accession #    Q969K3 [Other Products]
 Molecular Weight    41,797 Da
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 NCBI Official Full Name    E3 ubiquitin-protein ligase RNF34 isoform 2
 NCBI Official Synonym Full Names    ring finger protein 34, E3 ubiquitin protein ligase
 NCBI Official Symbol    RNF34 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    E3 ubiquitin-protein ligase RNF34; caspase regulator CARP1; RING finger protein RIFF; FYVE-RING finger protein MOMO; caspases-8 and -10-associated RING finger protein 1; human RING finger homologous to inhibitor of apoptosis protein
 UniProt Protein Name    E3 ubiquitin-protein ligase RNF34
 UniProt Synonym Protein Names   
Caspase regulator CARP1; FYVE-RING finger protein Momo; Human RING finger homologous to inhibitor of apoptosis protein; RING finger protein 34; RING finger protein RIFF
 Protein Family    E3 ubiquitin-protein ligase
 UniProt Gene Name    RNF34 [Similar Products]
 UniProt Entry Name    RNF34_HUMAN
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 NCBI Summary for RNF34    The protein encoded by this gene contains a RINF finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein interacts with DNAJA3/hTid-1, which is a DnaJ protein reported to function as a modulator of apoptosis. Overexpression of this gene in Hela cells was shown to confer the resistance to TNF-alpha induced apoptosis, suggesting an anti-apoptotic function of this protein. This protein can be cleaved by caspase-3 during the induction of apoptosis. This protein also targets p53 and phospho-p53 for degradation. Alternatively splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2012]
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 UniProt Comments for RNF34    RNF34: Has E3 ubiquitin-protein ligase activity. Regulates the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. Protects cells against apoptosis induced by TNF. Binds phosphatidylinositol 5-phosphate and phosphatidylinositol 3- phosphate. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.-; Ubiquitin ligase; Ligase; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: cytoplasm; plasma membrane; endomembrane system; nuclear speck; nucleus; cytosol

Molecular Function: protein binding; zinc ion binding; p53 binding; ubiquitin protein ligase binding; ligase activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein ubiquitination during ubiquitin-dependent protein catabolic process; protein ubiquitination
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 Research Articles on RNF34    1. Data indicate AE induces caspase-8-mediated activation of mitochondrial death pathways by decreasing the stability of CARP mRNAs in a p53-independent manner.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-RNF34 antibodyPathways associated with anti-RNF34 antibody
 Reference Product  PubMed Publications
 DEDD antibody  >1 publications with RNF34 and DEDD
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Antigen Processing: Ubiquitination & Proteasome Degradation Pathway antibodies  Antigen Processing: Ubiquitination & Proteasome Degradation Pathway Diagram
 Class I MHC Mediated Antigen Processing & Presentation Pathway antibodies  Class I MHC Mediated Antigen Processing & Presentation Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
Diseases associated with anti-RNF34 antibodyOrgans/Tissues associated with anti-RNF34 antibody
 Disease Name  Pubmed Publications
 Colorectal Neoplasms Antibodies  >6 publications with RNF34 and Colorectal Neoplasms
 Carcinoma Antibodies  >3 publications with RNF34 and Carcinoma
 Necrosis Antibodies  >3 publications with RNF34 and Necrosis
 Nervous System Diseases Antibodies  >2 publications with RNF34 and Nervous System Diseases
 Colonic Neoplasms Antibodies  >2 publications with RNF34 and Colonic Neoplasms
 Memory Disorders Antibodies  >1 publications with RNF34 and Memory Disorders
 Congenital Abnormalities Antibodies  >1 publications with RNF34 and Congenital Abnormalities
 Adenocarcinoma Antibodies  >1 publications with RNF34 and Adenocarcinoma
 Neoplasm Metastasis Antibodies  >1 publications with RNF34 and Neoplasm Metastasis
 Inflammation Antibodies  >1 publications with RNF34 and Inflammation
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >8 publications with RNF34 and Brain
 Blood Antibodies  >4 publications with RNF34 and Blood
 Muscle Antibodies  >3 publications with RNF34 and Muscle
 Nerve Antibodies  >1 publications with RNF34 and Nerve
 Liver Antibodies  >1 publications with RNF34 and Liver
 Vascular Antibodies  >1 publications with RNF34 and Vascular
 Stomach Antibodies  >1 publications with RNF34 and Stomach
 Ovary Antibodies  >1 publications with RNF34 and Ovary
 Lymph Node Antibodies  >1 publications with RNF34 and Lymph Node
 Heart Antibodies  >1 publications with RNF34 and Heart
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