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anti-ROBO1 antibody :: Goat ROBO1/DUTT1 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS421220
Western Blot (WB)
Unit / Price
0.1 mg  /  $300 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

ROBO1/DUTT1, Polyclonal Antibody

 Also Known As   

Goat anti-ROBO1/DUTT1 (Internal) Antibody

 Product Synonym Names    ROBO1; DUTT1; roundabout, axon guidance receptor, homolog 1 (Drosophila); HGNC: 10249; FLJ21882; SAX3; axon guidance receptor; roundabout 1; MGC131599; MGC133277; ROBO1 antibody; DUTT1 antibody; roundabout; axon guidance receptor; homolog 1 (Drosophila) antibody; HGNC: 10249 antibody; FLJ21882 antibody; SAX3 antibody; axon guidance receptor antibody; roundabout 1 antibody; MGC131599 antibody; MGC133277 antibody; ROBO1 / DUTT1 (Internal)
 Product Gene Name   

anti-ROBO1 antibody

[Similar Products]
 Product Synonym Gene Name    DUTT1 [Similar Products]
 Antibody/Peptide Pairs    ROBO1 / DUTT1 peptide (MBS425260) is used for blocking the activity of ROBO1/DUTT1 antibody (MBS421220)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence    GDVDLSNKIN EMK
 OMIM    602430
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 Clonality    Polyclonal
 Host    Goat
 Species Reactivity    Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
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 Purity/Purification    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
 Form/Format    Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
 Concentration    100ug specific antibody in 200ul (lot specific)
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 Immunogen    Peptide with sequence C-GDVDLSNKINEMK, from the internal region of the protein sequence according to NP_002932.1; NP_598334.1; NP_001139316.1; NP_001139317.1.
 Epitope    Internal region
 Note    This antibody is expected to recognise all reported isoforms (NP_002932.1; NP_598334.1; NP_001139316.1; NP_001139317.1).
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 Preparation and Storage    Aliquot and store at -20 degree C. Minimize freezing and thawing.
 ISO Certification    Manufactured in an ISO 9001:2015 Certified Laboratory.
 Other Notes    Small volumes of anti-ROBO1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-ROBO1 antibody   

Peptide ELISA (EIA), Western Blot (WB)

 Application Notes for anti-ROBO1 antibody    Peptide ELISA: Antibody detection limit dilution 1: 8000.
Western Blot: Approx 250kDa band observed in Human Endothelial Cell line HUVEC lysates (calculated MW of 181kDa according to NP_002932.1). Some of the observed proteolytic breakdown products are also found in Human, Mouse and Rat Brain lysates. Recommended concentration: 1-3ug/ml.
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 Western Blot (WB) of anti-ROBO1 antibody    (1ug/ml) staining of HUVEC lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-ROBO1 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for ROBO1. It may not necessarily be applicable to this product.
 NCBI GI #    4506569
 NCBI GeneID    6091
 NCBI Accession #    NP_002932.1 [Other Products]
 NCBI Related Accession #    NP_598334.1; NP_001139316.1; NP_001139317.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_002941.3 [Other Products]
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 UniProt Secondary Accession #    Q1RMC7; Q7Z300; Q9BUS7; B2RXI1; D3DU36; E9PD49 [Other Products]
 UniProt Related Accession #    Q9Y6N7 [Other Products]
 Molecular Weight    169,661 Da
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 NCBI Official Full Name    roundabout homolog 1 isoform a
 NCBI Official Synonym Full Names    roundabout guidance receptor 1
 NCBI Official Symbol    ROBO1 [Similar Products]
 NCBI Official Synonym Symbols   
SAX3; DUTT1
[Similar Products]
 NCBI Protein Information    roundabout homolog 1
 UniProt Protein Name    Roundabout homolog 1
 UniProt Synonym Protein Names   
Deleted in U twenty twenty; H-Robo-1
 Protein Family    Roundabout
 UniProt Gene Name    ROBO1 [Similar Products]
 UniProt Synonym Gene Names    DUTT1 [Similar Products]
 UniProt Entry Name    ROBO1_HUMAN
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 NCBI Summary for ROBO1    Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
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 UniProt Comments for ROBO1    ROBO1: Receptor for SLIT1 and SLIT2 which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. May be required for lung development. Belongs to the immunoglobulin superfamily. ROBO family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 3p12

Cellular Component: axon; cell surface; cytoplasm; integral to plasma membrane; plasma membrane

Molecular Function: axon guidance receptor activity; identical protein binding; LRR domain binding; protein binding

Biological Process: axon guidance; axon midline choice point recognition; caspase activation; cell adhesion; cell migration during sprouting angiogenesis; chemorepulsion involved in postnatal olfactory bulb interneuron migration; heart development; homophilic cell adhesion; negative regulation of cell migration; negative regulation of mammary gland epithelial cell proliferation; negative regulation of negative chemotaxis; nervous system development; positive regulation of axonogenesis; positive regulation of Rho protein signal transduction
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 Research Articles on ROBO1    1. Robo1 promoted cell division cycle 42 (Cdc42) expression in HUVECs, and a distorted actin cytoskeleton in HUVECs was observed when Robo1 expression was suppressed. In conclusion, Robo1 promoted angiogenesis in HCC mediated by Cdc42.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-ROBO1 antibodyPathways associated with anti-ROBO1 antibody
 Reference Product  PubMed Publications
 SLIT2 antibody  >150 publications with ROBO1 and SLIT2
 ROBO2 antibody  >98 publications with ROBO1 and ROBO2
 SLIT1 antibody  >64 publications with ROBO1 and SLIT1
 SLIT3 antibody  >45 publications with ROBO1 and SLIT3
 CDC42 antibody  >16 publications with ROBO1 and CDC42
 SRGAP1 antibody  >5 publications with ROBO1 and SRGAP1
 SRGAP2 antibody  >1 publications with ROBO1 and SRGAP2
 Products by Pathway  Pathway Diagram
 Activation Of Rac Pathway antibodies  Activation Of Rac Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Inactivation Of Cdc42 And Rac Pathway antibodies  Inactivation Of Cdc42 And Rac Pathway Diagram
 Netrin-1 Signaling Pathway antibodies  Netrin-1 Signaling Pathway Diagram
 Regulation Of Commissural Axon Pathfinding By Slit And Robo Pathway antibodies  Regulation Of Commissural Axon Pathfinding By Slit And Robo Pathway Diagram
 Role Of Abl In Robo-Slit Signaling Pathway antibodies  Role Of Abl In Robo-Slit Signaling Pathway Diagram
 Signaling By Robo Receptor Pathway antibodies  Signaling By Robo Receptor Pathway Diagram
Diseases associated with anti-ROBO1 antibodyOrgans/Tissues associated with anti-ROBO1 antibody
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >54 publications with ROBO1 and Carcinoma
 Learning Disorders Antibodies  >29 publications with ROBO1 and Learning Disorders
 Neurobehavioral Manifestations Antibodies  >28 publications with ROBO1 and Neurobehavioral Manifestations
 Neoplasm Invasiveness Antibodies  >19 publications with ROBO1 and Neoplasm Invasiveness
 Lung Diseases Antibodies  >18 publications with ROBO1 and Lung Diseases
 Adenocarcinoma Antibodies  >18 publications with ROBO1 and Adenocarcinoma
 Neoplasm Metastasis Antibodies  >17 publications with ROBO1 and Neoplasm Metastasis
 Disease Models, Animal Antibodies  >15 publications with ROBO1 and Disease Models, Animal
 Breast Neoplasms Antibodies  >14 publications with ROBO1 and Breast Neoplasms
 Lung Neoplasms Antibodies  >14 publications with ROBO1 and Lung Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >103 publications with ROBO1 and Brain
 Embryonic Tissue Antibodies  >64 publications with ROBO1 and Embryonic Tissue
 Vascular Antibodies  >48 publications with ROBO1 and Vascular
 Blood Antibodies  >38 publications with ROBO1 and Blood
 Lung Antibodies  >25 publications with ROBO1 and Lung
 Eye Antibodies  >25 publications with ROBO1 and Eye
 Liver Antibodies  >15 publications with ROBO1 and Liver
 Muscle Antibodies  >12 publications with ROBO1 and Muscle
 Ovary Antibodies  >7 publications with ROBO1 and Ovary
 Heart Antibodies  >7 publications with ROBO1 and Heart
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