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anti-ROBO3 antibody :: Goat ROBO3/RIG1 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS420646
Western Blot (WB)
Unit / Price
0.1 mg  /  $300 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

ROBO3/RIG1, Polyclonal Antibody

 Also Known As   

Goat anti-ROBO3/RIG1 (internal) Antibody

 Product Synonym Names    Goat Anti-ROBO3 (internal) Antibody; ROBO3; RIG1; roundabout, axon guidance receptor, homolog 3 (Drosophila); FLJ21044; HGPPS; RBIG1; retinoblastoma inhibiting gene 1; roundabout, axon guidance receptor, homolog 3; ROBO3 antibody; RIG1 antibody; roundabout; axon guidance receptor; homolog 3 (Drosophila) antibody; FLJ21044 antibody; HGPPS antibody; RBIG1 antibody; retinoblastoma inhibiting gene 1 antibody; roundabout; axon guidance receptor; homolog 3 antibody; ROBO3 / RIG1 (internal)
 Product Gene Name   

anti-ROBO3 antibody

[Similar Products]
 Product Synonym Gene Name    RIG1 [Similar Products]
 Antibody/Peptide Pairs    ROBO3 / RIG1 peptide (MBS425619) is used for blocking the activity of ROBO3/RIG1 antibody (MBS420646)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence    RRKQRKELSH YT
 OMIM    607313
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 Clonality    Polyclonal
 Host    Goat
 Species Reactivity    Tested: Human; Expected from sequence similarity: Human, Dog, Pig, Cow
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 Purity/Purification    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
 Form/Format    Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
 Concentration    100ug specific antibody in 200ul (lot specific)
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 Immunogen    Peptide with sequence C-RRKQRKELSHYT, from the internal region of the protein sequence according to NP_071765.2.
 Epitope    Internal region
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 Preparation and Storage    Aliquot and store at -20 degree C. Minimize freezing and thawing.
 ISO Certification    Manufactured in an ISO 9001:2015 Certified Laboratory.
 Other Notes    Small volumes of anti-ROBO3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-ROBO3 antibody   

Peptide ELISA (EIA), Western Blot (WB)

 Application Notes for anti-ROBO3 antibody    Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 150kDa band observed in Human Brain (Cerebellum and Hippocampus) lysates (calculated MW of 148kDa according to NP_071765.2). Recommended concentration: 0.5-1ug/ml.
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 Western Blot (WB) of anti-ROBO3 antibody    (0.5ug/ml) staining of Human Brain (Cerebellum) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-ROBO3 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for ROBO3. It may not necessarily be applicable to this product.
 NCBI GI #    48476182
 NCBI GeneID    64221
 NCBI Accession #    NP_071765.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_022370.3 [Other Products]
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 UniProt Related Accession #    Q96MS0 [Other Products]
 Molecular Weight    110,676 Da
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 NCBI Official Full Name    roundabout homolog 3
 NCBI Official Synonym Full Names    roundabout guidance receptor 3
 NCBI Official Symbol    ROBO3 [Similar Products]
 NCBI Official Synonym Symbols   
HGPS; RIG1; HGPPS; RBIG1
[Similar Products]
 NCBI Protein Information    roundabout homolog 3
 UniProt Protein Name    Roundabout homolog 3
 UniProt Synonym Protein Names   
Roundabout-like protein 3
 Protein Family    Roundabout
 UniProt Gene Name    ROBO3 [Similar Products]
 UniProt Entry Name    ROBO3_HUMAN
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 NCBI Summary for ROBO3    This gene is a member of the Roundabout (ROBO) gene family that controls neurite outgrowth, growth cone guidance, and axon fasciculation. ROBO proteins are a subfamily of the immunoglobulin transmembrane receptor superfamily. SLIT proteins 1-3, a family of secreted chemorepellants, are ligands for ROBO proteins and SLIT/ROBO interactions regulate myogenesis, leukocyte migration, kidney morphogenesis, angiogenesis, and vasculogenesis in addition to neurogenesis. This gene, ROBO3, has a putative extracellular domain with five immunoglobulin (Ig)-like loops and three fibronectin (Fn) type III motifs, a transmembrane segment, and a cytoplasmic tail with three conserved signaling motifs: CC0, CC2, and CC3 (CC for conserved cytoplasmic). Unlike other ROBO family members, ROBO3 lacks motif CC1. The ROBO3 gene regulates axonal navigation at the ventral midline of the neural tube. In mouse, loss of Robo3 results in a complete failure of commissural axons to cross the midline throughout the spinal cord and the hindbrain. Mutations ROBO3 result in horizontal gaze palsy with progressive scoliosis (HGPPS); an autosomal recessive disorder characterized by congenital absence of horizontal gaze, progressive scoliosis, and failure of the corticospinal and somatosensory axon tracts to cross the midline in the medulla. Alternative transcript variants have been described but have not been experimentally validated. [provided by RefSeq, Dec 2009]
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 UniProt Comments for ROBO3    ROBO3: Thought to be involved during neural development in axonal navigation at the ventral midline of the neural tube. In spinal chord development plays a role in guiding commissural axons probably by preventing premature sensitivity to Slit proteins thus inhibiting Slit signaling through ROBO1. Required for hindbrain axon midline crossing. Defects in ROBO3 are a cause of familial horizontal gaze palsy with progressive scoliosis (HGPPS). Patients show a medulla where motor and sensory projections appear uncrossed. Belongs to the immunoglobulin superfamily. ROBO family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q24.2

Cellular Component: axon; integral to membrane

Biological Process: axon guidance; axon midline choice point recognition

Disease: Gaze Palsy, Familial Horizontal, With Progressive Scoliosis
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 Research Articles on ROBO3    1. confirmed that ROBO3 increases with clinical grade and miR-383 expression is inversely correlated to that of ROBO3
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-ROBO3 antibodyPathways associated with anti-ROBO3 antibody
 Reference Product  PubMed Publications
 ROBO1 antibody  >29 publications with ROBO3 and ROBO1
 SLIT1 antibody  >21 publications with ROBO3 and SLIT1
 SLIT2 antibody  >16 publications with ROBO3 and SLIT2
 SLIT3 antibody  >11 publications with ROBO3 and SLIT3
 CHN1 antibody  >1 publications with ROBO3 and CHN1
 Products by Pathway  Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Regulation Of Commissural Axon Pathfinding By Slit And Robo Pathway antibodies  Regulation Of Commissural Axon Pathfinding By Slit And Robo Pathway Diagram
 Signaling By Robo Receptor Pathway antibodies  Signaling By Robo Receptor Pathway Diagram
Diseases associated with anti-ROBO3 antibodyOrgans/Tissues associated with anti-ROBO3 antibody
 Disease Name  Pubmed Publications
 Nervous System Malformations Antibodies  >9 publications with ROBO3 and Nervous System Malformations
 Carcinoma Antibodies  >7 publications with ROBO3 and Carcinoma
 Inflammation Antibodies  >6 publications with ROBO3 and Inflammation
 Brain Diseases Antibodies  >6 publications with ROBO3 and Brain Diseases
 Gaze Palsy, Familial Horizontal, with Progressive Scoliosis Antibodies  >6 publications with ROBO3 and Gaze Palsy, Familial Horizontal, with Progressive Scoliosis
 Lung Diseases Antibodies  >5 publications with ROBO3 and Lung Diseases
 Necrosis Antibodies  >5 publications with ROBO3 and Necrosis
 Liver Diseases Antibodies  >3 publications with ROBO3 and Liver Diseases
 Disease Models, Animal Antibodies  >3 publications with ROBO3 and Disease Models, Animal
 Neoplasm Metastasis Antibodies  >2 publications with ROBO3 and Neoplasm Metastasis
 Organ/Tissue Name  Pubmed Publications
 Nerve Antibodies  >145 publications with ROBO3 and Nerve
 Brain Antibodies  >59 publications with ROBO3 and Brain
 Embryonic Tissue Antibodies  >30 publications with ROBO3 and Embryonic Tissue
 Eye Antibodies  >23 publications with ROBO3 and Eye
 Lung Antibodies  >5 publications with ROBO3 and Lung
 Bone Marrow Antibodies  >4 publications with ROBO3 and Bone Marrow
 Bone Antibodies  >4 publications with ROBO3 and Bone
 Vascular Antibodies  >4 publications with ROBO3 and Vascular
 Ovary Antibodies  >3 publications with ROBO3 and Ovary
 Kidney Antibodies  >3 publications with ROBO3 and Kidney
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