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anti-SF3B3 antibody :: Goat SAP130/SF3B3 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS421368
Western Blot (WB)
Unit / Price
0.1 mg  /  $300 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

SAP130/SF3B3, Polyclonal Antibody

 Also Known As   

Goat anti-SAP130/SF3B3 Antibody

 Product Synonym Names    SF3B3; SAP130; splicing factor 3b, subunit 3, 130kDa; RSE1; SF3B130; KIAA0017; STAF130; pre-mRNA splicing factor SF3b, 130 kDa subunit; spliceosome-associated protein 130; splicing factor 3b, subunit 3; SF3b130; SF3B3 antibody; SAP130 antibody; splicing factor 3b; subunit 3; 130kDa antibody; RSE1 antibody; SF3B130 antibody; KIAA0017 antibody; STAF130 antibody; pre-mRNA splicing factor SF3b; 130 kDa subunit antibody; spliceosome-associated protein 130 antibody; splicing factor 3b; subunit 3 antibody; SF3b130 antibody; SAP130 / SF3B3
 Product Gene Name   

anti-SF3B3 antibody

[Similar Products]
 Product Synonym Gene Name    SAP130 [Similar Products]
 Antibody/Peptide Pairs    SAP130 / SF3B3 peptide (MBS425440) is used for blocking the activity of SAP130/SF3B3 antibody (MBS421368)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence    VSKKLEDIRT RYAF
 OMIM    605592
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 Clonality    Polyclonal
 Host    Goat
 Species Reactivity    Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
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 Purity/Purification    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
 Form/Format    Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
 Concentration    100ug specific antibody in 200ul (lot specific)
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 Immunogen    Peptide with sequence C-VSKKLEDIRTRYAF, from the C Terminus of the protein sequence according to NP_036558.3.
 Epitope    C Terminus
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 Preparation and Storage    Aliquot and store at -20 degree C. Minimize freezing and thawing.
 ISO Certification    Manufactured in an ISO 9001:2015 Certified Laboratory.
 Other Notes    Small volumes of anti-SF3B3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-SF3B3 antibody   

Peptide ELISA (EIA), Western Blot (WB)

 Application Notes for anti-SF3B3 antibody    Peptide ELISA: Antibody detection limit dilution 1: 2000.
Western Blot: Approx 150kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 136kDa according to NP_036558.3). Recommended concentration: 0.1-0.3ug/ml.
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 Western Blot (WB) of anti-SF3B3 antibody    Staining (0.1ug/ml) of nuclear lysate from HeLa cells (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
anti-SF3B3 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for SF3B3. It may not necessarily be applicable to this product.
 NCBI GI #    54112121
 NCBI GeneID    23450
 NCBI Accession #    NP_036558.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_012426.4 [Other Products]
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 UniProt Secondary Accession #    Q6NTI8; Q96GC0; Q9BPY2; Q9UFX7; Q9UJ29 [Other Products]
 UniProt Related Accession #    Q15393 [Other Products]
 Molecular Weight    44,606 Da
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 NCBI Official Full Name    splicing factor 3B subunit 3
 NCBI Official Synonym Full Names    splicing factor 3b subunit 3
 NCBI Official Symbol    SF3B3 [Similar Products]
 NCBI Official Synonym Symbols   
RSE1; SAP130; SF3b130; STAF130
[Similar Products]
 NCBI Protein Information    splicing factor 3B subunit 3
 UniProt Protein Name    Splicing factor 3B subunit 3
 UniProt Synonym Protein Names   
Pre-mRNA-splicing factor SF3b 130 kDa subunit; SF3b130; STAF130; Spliceosome-associated protein 130; SAP 130
 Protein Family    Splicing factor
 UniProt Gene Name    SF3B3 [Similar Products]
 UniProt Synonym Gene Names    KIAA0017; SAP130; SF3b130; SAP 130 [Similar Products]
 UniProt Entry Name    SF3B3_HUMAN
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 NCBI Summary for SF3B3    This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair. [provided by RefSeq, Jul 2008]
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 UniProt Comments for SF3B3    SF3B3: Subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron. Belongs to the RSE1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA splicing; Spliceosome; RNA-binding

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: nucleolus; nucleoplasm; nucleus; small nuclear ribonucleoprotein complex; spliceosome; U12-dependent spliceosome

Molecular Function: protein binding; RNA binding

Biological Process: mRNA processing; negative regulation of protein catabolic process; nuclear mRNA splicing, via spliceosome; protein complex assembly; RNA splicing; RNA splicing, via transesterification reactions
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 Research Articles on SF3B3    1. our findings suggest a role for the elevated expression of SF3B3 in poor prognosis and tamoxifen resistance, leading to recurrence of estrogen receptor-positive breast cancer
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-SF3B3 antibodyDiseases associated with anti-SF3B3 antibody
 Products by Pathway  Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Processing Of Capped Intron-Containing Pre-mRNA Pathway antibodies  Processing Of Capped Intron-Containing Pre-mRNA Pathway Diagram
 Spliceosome Pathway antibodies  Spliceosome Pathway Diagram
 Spliceosome Pathway antibodies  Spliceosome Pathway Diagram
 Spliceosome, U2-snRNP Pathway antibodies  Spliceosome, U2-snRNP Pathway Diagram
 Spliceosome, U2-snRNP Pathway antibodies  Spliceosome, U2-snRNP Pathway Diagram
 MRNA Splicing Pathway antibodies  MRNA Splicing Pathway Diagram
 MRNA Splicing - Major Pathway antibodies  MRNA Splicing - Major Pathway Diagram
 MRNA Splicing - Minor Pathway antibodies  MRNA Splicing - Minor Pathway Diagram
 MRNA Processing Pathway antibodies  MRNA Processing Pathway Diagram
 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >2 publications with SF3B3 and Breast Neoplasms
 Chromosome Aberrations Antibodies  >1 publications with SF3B3 and Chromosome Aberrations
 Abnormalities, Multiple Antibodies  >1 publications with SF3B3 and Abnormalities, Multiple
 Adenocarcinoma Antibodies  >1 publications with SF3B3 and Adenocarcinoma
 Cell Transformation, Neoplastic Antibodies  >1 publications with SF3B3 and Cell Transformation, Neoplastic
 Kidney Diseases Antibodies  >1 publications with SF3B3 and Kidney Diseases
Organs/Tissues associated with anti-SF3B3 antibody
 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >1 publications with SF3B3 and Vascular
 Lymph Node Antibodies  >1 publications with SF3B3 and Lymph Node
 Blood Antibodies  >1 publications with SF3B3 and Blood
 Mouth Antibodies  >1 publications with SF3B3 and Mouth
 Lymph Antibodies  >1 publications with SF3B3 and Lymph
 Kidney Antibodies  >1 publications with SF3B3 and Kidney
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