Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow Antibody rightarrow SERPINA1 rightarrow Polyclonal SERPINA1  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • CD109 Antibody
 • Colony Stimulating Factor 2 Receptor Alpha (CSF2Ra) ELISA Kit
 • PRMT5 Antibody
 • D-Glucuronic acid, free acid Biochemical
 • WASP Antibody
 • Interferon regulatory factor 6 (IRF6) Recombinant Protein
 • EGFR Antibody
 • Platelet Derived Growth Factor AA (PDGFAA) Antibody
 • Quantitative Cellular Senescence Assay Assay Kit
 • alpha2-M ELISA Kit
 • FSBA Antibody
 • A Proliferation inducing ligand (APRIL) ELISA Kit
 • CDH4 Antibody
 • Tumor Necrosis Factor alpha (TNFalpha) ELISA Kit
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

anti-SERPINA1 antibody :: Goat anti-Human SERPINA1 / Alpha 1 Antitrypsin Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS241593
Unit / Price
0.05 mL  /  $530 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

SERPINA1 / Alpha 1 Antitrypsin, Polyclonal Antibody

 Also Known As   

Goat Polyclonal to Human SERPINA1 / Alpha 1 Antitrypsin

 Product Synonym Names    Anti-SERPINA1 / Alpha 1 Antitrypsin Antibody IHC-plus; SERPINA1; A1A; A1AT; AAT; Alpha 1 Antitrypsin; Alpha-1 protease inhibitor; Alpha-1-antiproteinase; Alpha-1-antitrypsin; Alpha1AT; PRO2275; Alpha-1-antitrypsin null; PI1; Serpin A1; Human SERPINA1; Alpha 1 Antitrypsin
 Product Gene Name   

anti-SERPINA1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
 OMIM    gene 613490
 3D Structure    ModBase 3D Structure for P01009
Section Bar
 Clonality    Polyclonal
 Host    Goat
 Species Reactivity    Human
Section Bar
 Specificity    A1-Anti-Trypsin [Human Plasma].
 Purity/Purification    Purified IgG
 Form/Format    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide
 Concentration    10 mg/ml (lot specific)
Section Bar
 Target Species    Human
 Immunogen Description    Purified human SERPINA1 / Alpha-1-Antitrypsin.
 Immunogen    SERPINA1 / Alpha 1 Antitrypsin antibody was raised against purified human SERPINA1 / Alpha-1-Antitrypsin.
Section Bar
 Preparation and Storage    +4 degree C or -20 degree C, Avoid repeated freezing and thawing.
 Other Notes    Small volumes of anti-SERPINA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Table BarTOPTable Bar
 Applications Tested/Suitable for anti-SERPINA1 antibody   

Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), Immunoprecipitation (IP), ELISA (EIA)

 Application Notes for anti-SERPINA1 antibody    ELISA, IF, IHC-P (5 ug/ml), IP, WB
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
Section Bar
 Immunohistochemistry (IHC) of anti-SERPINA1 antibody    Anti-Alpha-1-Antitrypsin antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
anti-SERPINA1 antibody Immunohistochemistry (IHC) (IHC) image
Section Bar
 Western Blot (WB) of anti-SERPINA1 antibody    Alpha 1 anti-trypsin antibody-Fluorescent western blot. Primary and Dylight conjugated secondary antibodies were used to detect: Human transferrin; Alpha 1 anti-trypsin; and Human IgG in a multiplex fluorescent western blot of human serum. Each primary antibody was diluted to 1:1000 in IRdye blocking buffer (MB-070) and incubated for 2 hrs at RT. Blot was washed 3X in TTBS, 1X in TBS and probed with secondary antibodies diluted 1:10000) in IRdye blocking buffer and incubated ~1hr at 4 degrees. After wash 2X in TTBS and 2X in TBS, blot was rinsed 2X in MeOH, dried and imaged using the Bio-Rad VersaDoc4000.
anti-SERPINA1 antibody Western Blot (WB) (WB) image
Section Bar
 Western Blot (WB) of anti-SERPINA1 antibody    Alpha-1anti-Trypsin Polyclonal Antibody-Western blot. Goat anti-Alpha-1anti-Trypsin antibody (lot 5854) was used to detect Alpha-1anti-Trypsin under reducing conditions. Reduced sample of purified target protein contained 4% BME and were boiled for 5 minutes. Samples of ~1 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 488 conjugated Donkey anti-goat (1:10K in TBS/MB-070 1 hr RT). Images were collected using the Bio-Rad VersaDoc System.
anti-SERPINA1 antibody Western Blot (WB) (WB) image
Section Bar
 Western Blot (WB) of anti-SERPINA1 antibody    Alpha-1-Anti-Trypsin Polyclonal Antibody-Multiplex Fluorescent Western blot. Goat-anti-Alpha-1-Anti-Trypsin, Rabbit anti-Transferrin, and Mouse-a-GST were used in a multiplex system to detect target proteins under reducing (R) conditions (+4% BME) in albumin depleted human serum with 320 ng of added GST. Sample was run by SDS-PAGE, transferred to 0.2 um PVDF using the BioRad Trans-Blot Turbo and blocked in 2.5% Blotto, 2.5% BSA, 0.02% Tween over night at 4C. Membrane was probed with three primary antibodies at 1:1000 dilution (in MB-070 over night at 4C). Detection shown was using DyLight549 Donkey anti-Rabbit IgG (green) DyLight 488 Donkey anti-Mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) at 1:10000 (in MB-070 30 min RT). Blots were washed, rinsed in methanol, dried and Images were collected using the Bio-Rad VersaDoc System.
anti-SERPINA1 antibody Western Blot (WB) (WB) image
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for SERPINA1. It may not necessarily be applicable to this product.
 NCBI GI #    50363217
 NCBI GeneID    5265
 NCBI Accession #    NP_000286.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_000295.4 [Other Products]
Section Bar
 UniProt Primary Accession #    P01009 [Other Products]
 UniProt Secondary Accession #    Q0PVP5; Q13672; Q53XB8; Q5U0M1; Q7M4R2; Q86U18; Q86U19; Q96BF9; Q96ES1; A6PX14; B2RDQ8 [Other Products]
 UniProt Related Accession #    P01009 [Other Products]
 Molecular Weight    34,755 Da
Table BarTOPTable Bar
 NCBI Official Full Name    alpha-1-antitrypsin
 NCBI Official Synonym Full Names    serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
 NCBI Official Symbol    SERPINA1 [Similar Products]
 NCBI Official Synonym Symbols   
PI; A1A; AAT; PI1; A1AT; PRO2275; alpha1AT
[Similar Products]
 NCBI Protein Information    alpha-1-antitrypsin; serpin A1; alpha-1-antiproteinase; alpha-1-antitrypsin null; alpha-1 protease inhibitor; protease inhibitor 1 (anti-elastase), alpha-1-antitrypsin; serine (or cysteine) proteinase inhibitor, clade A, member 1
 UniProt Protein Name    Alpha-1-antitrypsin
 UniProt Synonym Protein Names   
Alpha-1 protease inhibitor; Alpha-1-antiproteinase; Serpin A1Short peptide from AAT; SPAAT
 Protein Family    Alpha-1-antitrypsin
 UniProt Gene Name    SERPINA1 [Similar Products]
 UniProt Synonym Gene Names    AAT; PI; SPAAT [Similar Products]
 UniProt Entry Name    A1AT_HUMAN
Section Bar
 NCBI Summary for SERPINA1    The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Section Bar
 UniProt Comments for SERPINA1    SERPINA1: Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Defects in SERPINA1 are the cause of alpha-1-antitrypsin deficiency (A1ATD). A disorder whose most common manifestation is emphysema, which becomes evident by the third to fourth decade. A less common manifestation of the deficiency is liver disease, which occurs in children and adults, and may result in cirrhosis and liver failure. Environmental factors, particularly cigarette smoking, greatly increase the risk of emphysema at an earlier age. Belongs to the serpin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Inhibitor; Secreted

Chromosomal Location of Human Ortholog: 14q32.1

Cellular Component: Golgi apparatus; proteinaceous extracellular matrix; extracellular space; endoplasmic reticulum; extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; identical protein binding; protein binding; protease binding; glycoprotein binding

Biological Process: platelet activation; response to chromate; platelet degranulation; response to triglyceride; response to cytokine stimulus; response to lead ion; response to methanol; response to hypoxia; acute-phase response; response to lipopolysaccharide; blood coagulation; response to estradiol stimulus

Disease: Alpha-1-antitrypsin Deficiency; Pulmonary Disease, Chronic Obstructive
Table BarTOPTable Bar
 Research Articles on SERPINA1    1. Histomorphological findings support that PiMZ heterozygosity has an independent aggravating effect on liver fibrosis, even though the pathogenic effect of alcohol consumption is much stronger
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Pathways associated with anti-SERPINA1 antibodyDiseases associated with anti-SERPINA1 antibody
 Products by Pathway  Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 FOXA1 Transcription Factor Network Pathway antibodies  FOXA1 Transcription Factor Network Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Platelet Activation, Signaling And Aggregation Pathway antibodies  Platelet Activation, Signaling And Aggregation Pathway Diagram
 Platelet Degranulation Pathway antibodies  Platelet Degranulation Pathway Diagram
 Response To Elevated Platelet Cytosolic Ca2+ Pathway antibodies  Response To Elevated Platelet Cytosolic Ca2+ Pathway Diagram
 Disease Name  Pubmed Publications
 Lung Diseases Antibodies  >129 publications with SERPINA1 and Lung Diseases
 alpha 1-Antitrypsin Deficiency Antibodies  >128 publications with SERPINA1 and alpha 1-Antitrypsin Deficiency
 Liver Diseases Antibodies  >119 publications with SERPINA1 and Liver Diseases
 Pulmonary Disease, Chronic Obstructive Antibodies  >53 publications with SERPINA1 and Pulmonary Disease, Chronic Obstructive
 Pulmonary Emphysema Antibodies  >36 publications with SERPINA1 and Pulmonary Emphysema
 Inflammation Antibodies  >34 publications with SERPINA1 and Inflammation
 Liver Cirrhosis Antibodies  >29 publications with SERPINA1 and Liver Cirrhosis
 Carcinoma, Hepatocellular Antibodies  >21 publications with SERPINA1 and Carcinoma, Hepatocellular
 Hepatitis Antibodies  >18 publications with SERPINA1 and Hepatitis
 Liver Neoplasms Antibodies  >18 publications with SERPINA1 and Liver Neoplasms
Organs/Tissues associated with anti-SERPINA1 antibody
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >109 publications with SERPINA1 and Liver
 Blood Antibodies  >97 publications with SERPINA1 and Blood
 Lung Antibodies  >71 publications with SERPINA1 and Lung
 Skin Antibodies  >14 publications with SERPINA1 and Skin
 Vascular Antibodies  >11 publications with SERPINA1 and Vascular
 Heart Antibodies  >11 publications with SERPINA1 and Heart
 Kidney Antibodies  >10 publications with SERPINA1 and Kidney
 Bone Antibodies  >9 publications with SERPINA1 and Bone
 Pancreas Antibodies  >8 publications with SERPINA1 and Pancreas
 Muscle Antibodies  >8 publications with SERPINA1 and Muscle
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions