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anti-SIRT1 antibody :: Rabbit anti-Human SIRT1 / Sirtuin 1 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS241746
Immunohistochemistry (IHC)
Unit / Price
0.05 mL  /  $530 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

SIRT1 / Sirtuin 1, Polyclonal Antibody

 Also Known As   

Rabbit Polyclonal (IgG) to Human SIRT1 / Sirtuin 1

 Product Synonym Names    Anti-SIRT1 / Sirtuin 1 Antibody (C-Terminus) IHC-plus; SIRT1; SIR2-like protein 1; SIR2alpha; SIR2L1; Sirtuin 1; Sir2-like 1; Sirtuin type 1; HSIR2; HSIRT1; Human SIRT1; Sirtuin 1
 Product Gene Name   

anti-SIRT1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    604479
 3D Structure    ModBase 3D Structure for Q96EB6
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human
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 Specificity    Peptide mapping to the carboxy terminal domain of human SIRT1
 Purity/Purification    Affinity Purified
 Form/Format    Phosphate-buffered solution, pH 7.2, 0.09% sodium azide, 50% glycerol.
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 Target Species    Human
 Immunogen Description    Synthetic peptide from human SIRT1.
 Immunogen Type    Synthetic peptide
 Immunogen    SIRT1 / Sirtuin 1 antibody was raised against synthetic peptide from human SIRT1.
 Antigen Modification    C-Terminus
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 Preparation and Storage    Store at 4 degree C. Do not freeze.
 Other Notes    Small volumes of anti-SIRT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-SIRT1 antibody

   The SIRT family of genes are highly conserved from prokaryotes to eukaryotes and are involved in diverse processes including transcriptional regulation, cell cycle progression, DNA damage repair and aging. Sirtuins utilize NAD+ as a substrate to catalyze deacetylation of specific acetylated protein and histones. SIRT1 has been shown to be induced by caloric restriction (CR) in several tissues and SIRT1 mediated deacetylase activity attenuates B-amyloid content in the brain during CR.
 Product Categories/Family for anti-SIRT1 antibody    Family: ADP-Ribosyltransferase
 Applications Tested/Suitable for anti-SIRT1 antibody   

Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Immunoprecipitation (IP)

 Application Notes for anti-SIRT1 antibody    IHC-P (1:50), IP, WB
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
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 Immunohistochemistry (IHC) of anti-SIRT1 antibody    Anti-SIRT1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
anti-SIRT1 antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for SIRT1. It may not necessarily be applicable to this product.
 NCBI GI #    7657575
 NCBI GeneID    23411
 NCBI Accession #    NP_036370.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_012238.4 [Other Products]
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 UniProt Primary Accession #    Q96EB6 [Other Products]
 UniProt Secondary Accession #    Q2XNF6; Q5JVQ0; Q9GZR9; Q9Y6F0 [Other Products]
 UniProt Related Accession #    Q96EB6 [Other Products]
 Molecular Weight    61,066 Da
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 NCBI Official Full Name    NAD-dependent protein deacetylase sirtuin-1 isoform a
 NCBI Official Synonym Full Names    sirtuin 1
 NCBI Official Symbol    SIRT1 [Similar Products]
 NCBI Official Synonym Symbols   
SIR2L1
[Similar Products]
 NCBI Protein Information    NAD-dependent protein deacetylase sirtuin-1; hSIR2; hSIRT1; SIR2alpha; sir2-like 1; sirtuin type 1; SIR2-like protein 1; regulatory protein SIR2 homolog 1; NAD-dependent deacetylase sirtuin-1
 UniProt Protein Name    NAD-dependent protein deacetylase sirtuin-1
 UniProt Synonym Protein Names   
Regulatory protein SIR2 homolog 1; SIR2-like protein 1; hSIR2
 Protein Family    NAD-dependent protein deacetylase sirtuin
 UniProt Gene Name    SIRT1 [Similar Products]
 UniProt Synonym Gene Names    SIR2L1; hSIRT1; hSIR2; 75SirT1 [Similar Products]
 UniProt Entry Name    SIR1_HUMAN
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 NCBI Summary for SIRT1    This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008]
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 UniProt Comments for SIRT1    SIRT1: an NAD-dependent protein deacetylase that links transcriptional regulation directly to intracellular energetics and participates in the coordination of several separate cellular functions such as cell cycle, response to DNA damage, metobolism, apoptosis and autophagy. Deacetylates a broad range of transcription factors and coregulators, thereby regulating target gene expression positively and negatively. Serves as a sensor of the cytosolic ratio of NAD(+)/NADH which is altered by glucose deprivation and metabolic changes associated with caloric restriction. Essential in skeletal muscle cell differentiation and in response to low nutrients mediates the inhibitory effect on skeletal myoblast differentiation which also involves 5'-AMP-activated protein kinase (AMPK) and nicotinamide phosphoribosyltransferase (NAMPT). Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. Elevation of NAD(+)/NADP(+) ratio activates SIRT1. Recruited to LRH1 target gene promoters by NR0B2/SHP thereby stimulating histone H3 and H4 deacetylation leading to transcriptional repression. Implicated in regulation of adipogenesis and fat mobilization in white adipocytes by repression of PPARG. Involved in liver and muscle metabolism. Is involved in autophagy, presumably by deacetylating ATG5, ATG7 and ATG8. Deacetylates AKT1 which leads to enhanced binding of AKT1 and PDK1 to PIP3 and promotes their activation. Widely expressed. Inhibited by nicotinamide. Belongs to the sirtuin family. Class I subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.5.1.-; Apoptosis; Deacetylase; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 10q21.3

Cellular Component: nucleoplasm; chromatin silencing complex; PML body; mitochondrion; ESC/E(Z) complex; cytoplasm; nuclear chromatin; nucleolus; nuclear inner membrane; nuclear envelope; nuclear heterochromatin; nucleus

Molecular Function: deacetylase activity; protein C-terminus binding; identical protein binding; histone binding; p53 binding; metal ion binding; protein deacetylase activity; transcription factor binding; protein binding; NAD-dependent histone deacetylase activity (H3-K9 specific); enzyme binding; NAD-dependent histone deacetylase activity; bHLH transcription factor binding; mitogen-activated protein kinase binding; histone deacetylase activity; transcription corepressor activity; HLH domain binding; NAD+ ADP-ribosyltransferase activity

Biological Process: muscle development; establishment and/or maintenance of chromatin architecture; viral reproduction; positive regulation of apoptosis; regulation of mitotic cell cycle; positive regulation of caspase activity; protein ubiquitination; negative regulation of prostaglandin biosynthetic process; negative regulation of DNA damage response, signal transduction by p53 class mediator; negative regulation of gene expression, epigenetic; behavioral response to starvation; positive regulation of adaptive immune response; positive regulation of histone H3-K9 methylation; positive regulation of DNA repair; transcription, DNA-dependent; negative regulation of transcription factor activity; pyrimidine dimer repair via nucleotide-excision repair; cell aging; negative regulation of I-kappaB kinase/NF-kappaB cascade; ovulation from ovarian follicle; negative regulation of fat cell differentiation; cellular response to starvation; protein amino acid ADP-ribosylation; regulation of endodeoxyribonuclease activity; cholesterol homeostasis; methylation-dependent chromatin silencing; inhibition of NF-kappaB transcription factor; maintenance of chromatin silencing; regulation of protein import into nucleus, translocation; negative regulation of phosphorylation; positive regulation of transcription from RNA polymerase II promoter; response to oxidative stress; negative regulation of transcription, DNA-dependent; triacylglycerol mobilization; rRNA processing; peptidyl-lysine acetylation; negative regulation of apoptosis; chromatin silencing at rDNA; proteasomal ubiquitin-dependent protein catabolic process; establishment of chromatin silencing; chromatin silencing; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic; response to insulin stimulus; DNA synthesis during DNA repair; protein amino acid deacetylation; positive regulation of MHC class II biosynthetic process; positive regulation of cell proliferation; angiogenesis; circadian regulation of gene expression; DNA replication; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; single strand break repair; positive regulation of chromatin silencing; negative regulation of TOR signaling pathway; negative regulation of protein kinase B signaling cascade; protein destabilization; histone deacetylation; DNA repair; regulation of cell proliferation; negative regulation of helicase activity; response to hydrogen peroxide; fatty acid homeostasis; white fat cell differentiation; cell glucose homeostasis; spermatogenesis; gene expression; positive regulation of insulin receptor signaling pathway; negative regulation of cell growth; positive regulation of protein amino acid phosphorylation; negative regulation of transforming growth factor beta receptor signaling pathway; positive regulation of macroautophagy; response to DNA damage stimulus
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 Research Articles on SIRT1    1. Data suggest a role for SIRT1 in regulating autophagy and mitochondria function in embryonic stem cells (ESCs) upon oxidative stress.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-SIRT1 antibodyPathways associated with anti-SIRT1 antibody
 Reference Product  PubMed Publications
 PPARGC1A antibody  >158 publications with SIRT1 and PPARGC1A
 FOXO1 antibody  >155 publications with SIRT1 and FOXO1
 FOXO3 antibody  >68 publications with SIRT1 and FOXO3
 TP53 antibody  >54 publications with SIRT1 and TP53
 EP300 antibody  >17 publications with SIRT1 and EP300
 FOXO4 antibody  >12 publications with SIRT1 and FOXO4
 KAT5 antibody  >7 publications with SIRT1 and KAT5
 KAT2B antibody  >2 publications with SIRT1 and KAT2B
 FHL2 antibody  >1 publications with SIRT1 and FHL2
 XRCC6 antibody  >1 publications with SIRT1 and XRCC6
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 Amphetamine Addiction Pathway antibodies  Amphetamine Addiction Pathway Diagram
 Amphetamine Addiction Pathway antibodies  Amphetamine Addiction Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 Cellular Response To Heat Stress Pathway antibodies  Cellular Response To Heat Stress Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 E2F Transcription Factor Network Pathway antibodies  E2F Transcription Factor Network Pathway Diagram
 Energy Metabolism Pathway antibodies  Energy Metabolism Pathway Diagram
 Epigenetic Regulation Of Gene Expression Pathway antibodies  Epigenetic Regulation Of Gene Expression Pathway Diagram
Diseases associated with anti-SIRT1 antibodyOrgans/Tissues associated with anti-SIRT1 antibody
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >347 publications with SIRT1 and Inflammation
 Cardiovascular Diseases Antibodies  >328 publications with SIRT1 and Cardiovascular Diseases
 Obesity Antibodies  >285 publications with SIRT1 and Obesity
 Insulin Resistance Antibodies  >223 publications with SIRT1 and Insulin Resistance
 Neurodegenerative Diseases Antibodies  >184 publications with SIRT1 and Neurodegenerative Diseases
 Liver Diseases Antibodies  >178 publications with SIRT1 and Liver Diseases
 Fatty Liver Antibodies  >150 publications with SIRT1 and Fatty Liver
 Necrosis Antibodies  >135 publications with SIRT1 and Necrosis
 Heart Diseases Antibodies  >119 publications with SIRT1 and Heart Diseases
 Atherosclerosis Antibodies  >102 publications with SIRT1 and Atherosclerosis
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >415 publications with SIRT1 and Liver
 Muscle Antibodies  >409 publications with SIRT1 and Muscle
 Brain Antibodies  >313 publications with SIRT1 and Brain
 Vascular Antibodies  >268 publications with SIRT1 and Vascular
 Kidney Antibodies  >136 publications with SIRT1 and Kidney
 Lung Antibodies  >117 publications with SIRT1 and Lung
 Bone Antibodies  >104 publications with SIRT1 and Bone
 Pancreas Antibodies  >65 publications with SIRT1 and Pancreas
 Skin Antibodies  >65 publications with SIRT1 and Skin
 Eye Antibodies  >46 publications with SIRT1 and Eye
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