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anti-SIRT1 antibody :: Rabbit anti-Human, Rat SIRT1 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS125996
Unit / Price
0.05 mL  /  $230 +1 FREE 8GB USB
0.1 mL  /  $305 +1 FREE 8GB USB
0.2 mL  /  $460 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

SIRT1, Polyclonal Antibody

★Popular Item★
 Also Known As   

SIRT1 Polyclonal Antibody

 Product Synonym Names    SIRT1; SIR2L1
 Product Gene Name   

anti-SIRT1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    604479
 3D Structure    ModBase 3D Structure for Q96EB6
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human, Rat
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 Purity/Purification    Affinity Purification
 Concentration    1mg/ml (lot specific)
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 Species    Human
 Route    Synthetic Peptide
 Immunogen    C term -peptide of human SIRT1
 Calculated Molecular Weight    82kDa
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 Preparation and Storage    Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
 Other Notes    Small volumes of anti-SIRT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-SIRT1 antibody

   The Silent Information Regulator (SIR2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as class III histone deacetylases. The first discovered and best characterized of these genes is Saccharomyces cerevisiae SIR2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response, and cell aging (1). SirT1, the mammalian ortholog of Sir2, is a nuclear protein implicated in the regulation of many cellular processes, including apoptosis, cellular senescence, endocrine signaling, glucose homeostasis, aging, and longevity. Targets of SirT1 include acetylated p53 (2,3), p300 (4), Ku70 (5), forkhead (FoxO) transcription factors (5,6), PPARgamma (7), and the PPARgamma coactivator-1alpha (PGC-1alpha) protein (8). Deacetylation of p53 and FoxO transcription factors represses apoptosis and increases cell survival (2,3,5,6). Deacetylation of PPARgamma and PGC-1alpha regulates the gluconeogenic/glycolytic pathways in the liver and fat mobilization in white adipocytes in response to fasting (7,8). SirT1 deacetylase activity is inhibited by nicotinamide and activated by resveratrol. In addition, SirT1 activity may be regulated by phosphorylation, since it is phosphorylated on Ser27 and Ser47 in vivo, however, the function of these phosphorylation sites has not yet been determined (9).
 Product Categories/Family for anti-SIRT1 antibody    Polyclonal
 Applications Tested/Suitable for anti-SIRT1 antibody   

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), CHIP

 Application Notes for anti-SIRT1 antibody    WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:50 - 1:200
ChIP: 1:20 - 1:100
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 Immunohistochemistry (IHC) of anti-SIRT1 antibody    Immunohistochemistry of paraffin-embedded human stomach using SIRT1 antibody at dilution of 1:100 (40x lens).
anti-SIRT1 antibody Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of anti-SIRT1 antibody    Immunohistochemistry of paraffin-embedded mouse lung using SIRT1 antibody at dilution of 1:100 (40x lens).
anti-SIRT1 antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for SIRT1. It may not necessarily be applicable to this product.
 NCBI GI #    38258633
 NCBI GeneID    23411
 NCBI Accession #    Q96EB6.2 [Other Products]
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 UniProt Primary Accession #    Q96EB6 [Other Products]
 UniProt Secondary Accession #    Q2XNF6; Q5JVQ0; Q9GZR9; Q9Y6F0 [Other Products]
 UniProt Related Accession #    Q96EB6 [Other Products]
 Molecular Weight    747
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 NCBI Official Full Name    NAD-dependent protein deacetylase sirtuin-1
 NCBI Official Synonym Full Names    sirtuin 1
 NCBI Official Symbol    SIRT1 [Similar Products]
 NCBI Official Synonym Symbols   
SIR2L1
[Similar Products]
 NCBI Protein Information    NAD-dependent protein deacetylase sirtuin-1; hSIR2; hSIRT1; SIR2alpha; sir2-like 1; sirtuin type 1; SIR2-like protein 1; regulatory protein SIR2 homolog 1; NAD-dependent deacetylase sirtuin-1
 UniProt Protein Name    NAD-dependent protein deacetylase sirtuin-1
 UniProt Synonym Protein Names   
Regulatory protein SIR2 homolog 1; SIR2-like protein 1; hSIR2
 Protein Family    NAD-dependent protein deacetylase sirtuin
 UniProt Gene Name    SIRT1 [Similar Products]
 UniProt Synonym Gene Names    SIR2L1; hSIRT1; hSIR2; 75SirT1 [Similar Products]
 UniProt Entry Name    SIR1_HUMAN
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 NCBI Summary for SIRT1    This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008]
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 UniProt Comments for SIRT1    SIRT1: an NAD-dependent protein deacetylase that links transcriptional regulation directly to intracellular energetics and participates in the coordination of several separate cellular functions such as cell cycle, response to DNA damage, metobolism, apoptosis and autophagy. Deacetylates a broad range of transcription factors and coregulators, thereby regulating target gene expression positively and negatively. Serves as a sensor of the cytosolic ratio of NAD(+)/NADH which is altered by glucose deprivation and metabolic changes associated with caloric restriction. Essential in skeletal muscle cell differentiation and in response to low nutrients mediates the inhibitory effect on skeletal myoblast differentiation which also involves 5'-AMP-activated protein kinase (AMPK) and nicotinamide phosphoribosyltransferase (NAMPT). Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. Elevation of NAD(+)/NADP(+) ratio activates SIRT1. Recruited to LRH1 target gene promoters by NR0B2/SHP thereby stimulating histone H3 and H4 deacetylation leading to transcriptional repression. Implicated in regulation of adipogenesis and fat mobilization in white adipocytes by repression of PPARG. Involved in liver and muscle metabolism. Is involved in autophagy, presumably by deacetylating ATG5, ATG7 and ATG8. Deacetylates AKT1 which leads to enhanced binding of AKT1 and PDK1 to PIP3 and promotes their activation. Widely expressed. Inhibited by nicotinamide. Belongs to the sirtuin family. Class I subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Deacetylase; Apoptosis; EC 3.5.1.-; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 10q21.3

Cellular Component: nucleoplasm; chromatin silencing complex; PML body; mitochondrion; ESC/E(Z) complex; cytoplasm; nuclear chromatin; nucleolus; nuclear inner membrane; nuclear envelope; nuclear heterochromatin; nucleus

Molecular Function: protein C-terminus binding; deacetylase activity; identical protein binding; histone binding; p53 binding; metal ion binding; protein deacetylase activity; transcription factor binding; protein binding; NAD-dependent histone deacetylase activity (H3-K9 specific); enzyme binding; NAD-dependent histone deacetylase activity; bHLH transcription factor binding; mitogen-activated protein kinase binding; histone deacetylase activity; HLH domain binding; transcription corepressor activity; NAD+ ADP-ribosyltransferase activity

Biological Process: establishment and/or maintenance of chromatin architecture; muscle development; viral reproduction; positive regulation of apoptosis; regulation of mitotic cell cycle; protein ubiquitination; positive regulation of caspase activity; negative regulation of prostaglandin biosynthetic process; negative regulation of DNA damage response, signal transduction by p53 class mediator; negative regulation of gene expression, epigenetic; behavioral response to starvation; positive regulation of adaptive immune response; positive regulation of histone H3-K9 methylation; positive regulation of DNA repair; transcription, DNA-dependent; negative regulation of transcription factor activity; pyrimidine dimer repair via nucleotide-excision repair; cell aging; negative regulation of I-kappaB kinase/NF-kappaB cascade; ovulation from ovarian follicle; cellular response to starvation; negative regulation of fat cell differentiation; protein amino acid ADP-ribosylation; regulation of endodeoxyribonuclease activity; cholesterol homeostasis; methylation-dependent chromatin silencing; inhibition of NF-kappaB transcription factor; maintenance of chromatin silencing; regulation of protein import into nucleus, translocation; negative regulation of phosphorylation; positive regulation of transcription from RNA polymerase II promoter; response to oxidative stress; triacylglycerol mobilization; negative regulation of transcription, DNA-dependent; rRNA processing; peptidyl-lysine acetylation; negative regulation of apoptosis; chromatin silencing at rDNA; proteasomal ubiquitin-dependent protein catabolic process; establishment of chromatin silencing; chromatin silencing; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic; response to insulin stimulus; DNA synthesis during DNA repair; protein amino acid deacetylation; positive regulation of MHC class II biosynthetic process; positive regulation of cell proliferation; angiogenesis; circadian regulation of gene expression; DNA replication; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; positive regulation of chromatin silencing; single strand break repair; negative regulation of TOR signaling pathway; negative regulation of protein kinase B signaling cascade; protein destabilization; histone deacetylation; DNA repair; regulation of cell proliferation; negative regulation of helicase activity; response to hydrogen peroxide; fatty acid homeostasis; white fat cell differentiation; cell glucose homeostasis; gene expression; spermatogenesis; positive regulation of protein amino acid phosphorylation; positive regulation of insulin receptor signaling pathway; negative regulation of cell growth; negative regulation of transforming growth factor beta receptor signaling pathway; response to DNA damage stimulus; positive regulation of macroautophagy
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 Research Articles on SIRT1    1. The neuronal subcellular redistribution of SIRT1 in the entorhinal cortex and hippocampus parallels the decrease in its expression during progression of Alzheimer disease.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-SIRT1 antibodyPathways associated with anti-SIRT1 antibody
 Reference Product  PubMed Publications
 PPARGC1A antibody  >158 publications with SIRT1 and PPARGC1A
 FOXO1 antibody  >155 publications with SIRT1 and FOXO1
 FOXO3 antibody  >68 publications with SIRT1 and FOXO3
 TP53 antibody  >54 publications with SIRT1 and TP53
 EP300 antibody  >17 publications with SIRT1 and EP300
 FOXO4 antibody  >12 publications with SIRT1 and FOXO4
 KAT5 antibody  >7 publications with SIRT1 and KAT5
 KAT2B antibody  >2 publications with SIRT1 and KAT2B
 FHL2 antibody  >1 publications with SIRT1 and FHL2
 XRCC6 antibody  >1 publications with SIRT1 and XRCC6
 Products by Pathway  Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Amphetamine Addiction Pathway antibodies  Amphetamine Addiction Pathway Diagram
 Amphetamine Addiction Pathway antibodies  Amphetamine Addiction Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 Cellular Response To Heat Stress Pathway antibodies  Cellular Response To Heat Stress Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 E2F Transcription Factor Network Pathway antibodies  E2F Transcription Factor Network Pathway Diagram
 Energy Metabolism Pathway antibodies  Energy Metabolism Pathway Diagram
 Epigenetic Regulation Of Gene Expression Pathway antibodies  Epigenetic Regulation Of Gene Expression Pathway Diagram
Diseases associated with anti-SIRT1 antibodyOrgans/Tissues associated with anti-SIRT1 antibody
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >349 publications with SIRT1 and Inflammation
 Cardiovascular Diseases Antibodies  >328 publications with SIRT1 and Cardiovascular Diseases
 Obesity Antibodies  >285 publications with SIRT1 and Obesity
 Insulin Resistance Antibodies  >225 publications with SIRT1 and Insulin Resistance
 Neurodegenerative Diseases Antibodies  >185 publications with SIRT1 and Neurodegenerative Diseases
 Liver Diseases Antibodies  >178 publications with SIRT1 and Liver Diseases
 Fatty Liver Antibodies  >150 publications with SIRT1 and Fatty Liver
 Necrosis Antibodies  >135 publications with SIRT1 and Necrosis
 Heart Diseases Antibodies  >119 publications with SIRT1 and Heart Diseases
 Atherosclerosis Antibodies  >103 publications with SIRT1 and Atherosclerosis
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >415 publications with SIRT1 and Liver
 Muscle Antibodies  >409 publications with SIRT1 and Muscle
 Brain Antibodies  >314 publications with SIRT1 and Brain
 Vascular Antibodies  >268 publications with SIRT1 and Vascular
 Kidney Antibodies  >137 publications with SIRT1 and Kidney
 Lung Antibodies  >117 publications with SIRT1 and Lung
 Bone Antibodies  >104 publications with SIRT1 and Bone
 Pancreas Antibodies  >65 publications with SIRT1 and Pancreas
 Skin Antibodies  >65 publications with SIRT1 and Skin
 Eye Antibodies  >46 publications with SIRT1 and Eye
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