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anti-SLC9A3R1 antibody :: Rabbit SLC9A3R1 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS9210370
Unit / Price
0.08 mL  /  $150 +1 FREE 8GB USB
0.4 mL  /  $340 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

SLC9A3R1, Polyclonal Antibody

★Popular Item★
 Also Known As   

SLC9A3R1 Antibody (Center)

 Product Synonym Names    Na(+)/H(+) exchange regulatory cofactor NHE-RF1; NHERF-1; Ezrin-radixin-moesin-binding phosphoprotein 50; EBP50; Regulatory cofactor of Na(+)/H(+) exchanger; Sodium-hydrogen exchanger regulatory factor 1; Solute carrier family 9 isoform A3 regulatory factor 1; SLC9A3R1; NHERF; NHERF1
 Product Gene Name   

anti-SLC9A3R1 antibody

[Similar Products]
 Antibody/Peptide Pairs    SLC9A3R1 peptide (MBS9225811) is used for blocking the activity of SLC9A3R1 antibody (MBS9210370)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence Positions    168-197
 OMIM    604990
 3D Structure    ModBase 3D Structure for O14745
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 Clonality    Polyclonal
 Isotype    Rabbit Ig
 Host    Rabbit
 Species Reactivity    Human (Predicted Reactivity: Bovine, Monkey, Mouse, Rat)
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 Specificity    This SLC9A3R1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 168-197 amino acids from the Central region of human SLC9A3R1.
 Purity/Purification    Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
 Form/Format    Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
 Concentration    Vial Concentration: 0.5 (lot specific)
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 Antigen Type    Synthetic Peptide
 Antigen Source    HUMAN
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 Preparation and Storage    Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
 Other Notes    Small volumes of anti-SLC9A3R1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-SLC9A3R1 antibody

   Function: Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling. May participate in HTR4 targeting to microvilli (By similarity). Involved in the regulation of phosphate reabsorption in the renal proximal tubules. Involved in sperm capacitation. May participate in the regulation of the chloride and bicarbonate homeostasis in spermatozoa.

Cellular Location: Cytoplasm. Apical cell membrane. Endomembrane system; Peripheral membrane protein. Cell projection, filopodium. Cell projection, ruffle Cell projection, microvillus. Note=Translocates from the cytoplasm to the apical cell membrane in a PODXL-dependent manner Colocalizes with CFTR at the midpiece of sperm tail (By similarity). Colocalizes with actin in microvilli-rich apical regions of the syncytiotrophoblast. Found in microvilli, ruffling membrane and filopodia of HeLa cells. Present in lipid rafts of T- cells.

Tissue Location: Detected in liver, kidney, pancreas, prostate, spleen, small intestine and placenta, in particular in the syncytiotrophoblast.
 Product Categories/Family for anti-SLC9A3R1 antibody    Cardiovascular
 Applications Tested/Suitable for anti-SLC9A3R1 antibody   

Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)

 Application Notes for anti-SLC9A3R1 antibody    WB~~1:1000; FC~~1:10~50
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 Western Blot (WB) of anti-SLC9A3R1 antibody    All lanes : Anti-SLC9A3R1 Antibody (Center) at 1:2000 dilution
Lane 1: MCF-7 whole cell lysates
Lane 2: T47D whole cell lysates

Lysates/proteins at 20 ug per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

Predicted band size : 39 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
anti-SLC9A3R1 antibody Western Blot (WB) (WB) image
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 Western Blot (WB) of anti-SLC9A3R1 antibody    SLC9A3R1 Antibody (Center) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the SLC9A3R1 antibody detected the SLC9A3R1 protein (arrow).
anti-SLC9A3R1 antibody Western Blot (WB) (WB) image
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 Flow Cytometry (FC/FACS) of anti-SLC9A3R1 antibody    SLC9A3R1 Antibody (Center) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
anti-SLC9A3R1 antibody Flow Cytometry (FC/FACS) (FC/FACS) image
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NCBI/Uniprot data below describe general gene information for SLC9A3R1. It may not necessarily be applicable to this product.
 NCBI GI #    4759140
 NCBI GeneID    9368
 NCBI Accession #    NP_004243.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_004252.4 [Other Products]
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 UniProt Primary Accession #    O14745 [Other Products]
 UniProt Secondary Accession #    O43552; Q86WQ5; B3KY21 [Other Products]
 UniProt Related Accession #    O14745 [Other Products]
 Molecular Weight    38868
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 NCBI Official Full Name    Na(+)/H(+) exchange regulatory cofactor NHE-RF1
 NCBI Official Synonym Full Names    solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 1
 NCBI Official Symbol    SLC9A3R1 [Similar Products]
 NCBI Official Synonym Symbols   
EBP50; NHERF; NHERF1; NHERF-1; NPHLOP2
[Similar Products]
 NCBI Protein Information    Na(+)/H(+) exchange regulatory cofactor NHE-RF1
 UniProt Protein Name    Na(+)/H(+) exchange regulatory cofactor NHE-RF1
 UniProt Synonym Protein Names   
Ezrin-radixin-moesin-binding phosphoprotein 50; EBP50; Regulatory cofactor of Na(+)/H(+) exchanger; Sodium-hydrogen exchanger regulatory factor 1; Solute carrier family 9 isoform A3 regulatory factor 1
 Protein Family    Na(+)/H(+) exchange regulatory cofactor
 UniProt Gene Name    SLC9A3R1 [Similar Products]
 UniProt Synonym Gene Names    NHERF; NHERF1; NHERF-1; EBP50 [Similar Products]
 UniProt Entry Name    NHRF1_HUMAN
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 NCBI Summary for SLC9A3R1    This gene encodes a sodium/hydrogen exchanger regulatory cofactor. The protein interacts with and regulates various proteins including the cystic fibrosis transmembrane conductance regulator and G-protein coupled receptors such as the beta2-adrenergic receptor and the parathyroid hormone 1 receptor. The protein also interacts with proteins that function as linkers between integral membrane and cytoskeletal proteins. The protein localizes to actin-rich structures including membrane ruffles, microvilli, and filopodia. Mutations in this gene result in hypophosphatemic nephrolithiasis/osteoporosis type 2, and loss of heterozygosity of this gene is implicated in breast cancer.[provided by RefSeq, Sep 2009]
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 UniProt Comments for SLC9A3R1    NHERF: a scaffold protein that connects plasma membrane proteins with members of the Ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized beta-2-adrenergic receptor Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17q25.1

Cellular Component: centrosome; microvillus; intracellular membrane-bound organelle; brush border membrane; endomembrane system; lipid raft; actin cytoskeleton; ruffle; membrane; microvillus membrane; cytoplasm; apical plasma membrane; plasma membrane; nucleus; vesicle; filopodium

Molecular Function: protein binding; chloride channel regulator activity; protein self-association; beta-2 adrenergic receptor binding; protein complex binding; beta-catenin binding; dopamine receptor binding; protein complex scaffold; phosphatase binding; PDZ domain binding; receptor binding

Biological Process: bile acid secretion; negative regulation of protein kinase B signaling cascade; Wnt receptor signaling pathway; renal sodium ion transport; negative regulation of cell proliferation; microvillus biogenesis; cellular phosphate ion homeostasis; regulation of sodium:hydrogen antiporter activity; protein complex assembly; dopamine receptor, phospholipase C activating pathway; negative regulation of sodium:hydrogen antiporter activity; regulation of protein kinase activity; actin cytoskeleton organization and biogenesis; negative regulation of cell migration; negative regulation of phosphoinositide 3-kinase cascade; dopamine receptor, adenylate cyclase activating pathway

Disease: Nephrolithiasis/osteoporosis, Hypophosphatemic, 2
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 Research Articles on SLC9A3R1    1. PDZK1 and NHERF1 regulate the transport function of OATP1A2 by modulating protein internalization via a clathrin-dependent pathway and by enhancing protein stability.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-SLC9A3R1 antibodyPathways associated with anti-SLC9A3R1 antibody
 Reference Product  PubMed Publications
 CFTR antibody  >1 publications with SLC9A3R1 and CFTR
 Products by Pathway  Pathway Diagram
 Lysine Biosynthesis Pathway antibodies  Lysine Biosynthesis Pathway Diagram
 Lysine Biosynthesis Pathway antibodies  Lysine Biosynthesis Pathway Diagram
Diseases associated with anti-SLC9A3R1 antibodyOrgans/Tissues associated with anti-SLC9A3R1 antibody
 Disease Name  Pubmed Publications
 Immune System Diseases Antibodies  >5 publications with SLC9A3R1 and Immune System Diseases
 Inflammation Antibodies  >2 publications with SLC9A3R1 and Inflammation
 Cardiovascular Diseases Antibodies  >1 publications with SLC9A3R1 and Cardiovascular Diseases
 Fibrosis Antibodies  >1 publications with SLC9A3R1 and Fibrosis
 Liver Diseases Antibodies  >1 publications with SLC9A3R1 and Liver Diseases
 Liver Neoplasms Antibodies  >1 publications with SLC9A3R1 and Liver Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >3 publications with SLC9A3R1 and Bone
 Skin Antibodies  >2 publications with SLC9A3R1 and Skin
 Blood Antibodies  >2 publications with SLC9A3R1 and Blood
 Adipose Tissue Antibodies  >1 publications with SLC9A3R1 and Adipose Tissue
 Bone Marrow Antibodies  >1 publications with SLC9A3R1 and Bone Marrow
 Ovary Antibodies  >1 publications with SLC9A3R1 and Ovary
 Connective Tissue Antibodies  >1 publications with SLC9A3R1 and Connective Tissue
 Eye Antibodies  >1 publications with SLC9A3R1 and Eye
 Liver Antibodies  >1 publications with SLC9A3R1 and Liver
 Brain Antibodies  >1 publications with SLC9A3R1 and Brain
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