anti-SMURF1 antibody :: Rabbit anti-Human, Mouse SMURF1 Polyclonal Antibody
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Species Reactivity
Human, Mouse
Purity/Purification
>95%, Protein G purified
Immunogen
Recombinant human E3 ubiquitin-protein ligase SMURF1 protein (198-374AA)
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, PH 7.4
Conjugation
Non-conjugated
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-14906 / sc-14908 / sc-5731 / sc-5732 / sc-25510 / sc-130877
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-SMURF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for
anti-SMURF1 antibody
E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA. Plays a role in dendrite formation by melanocytes (PubMed: 23999003).
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Applications Tested/Suitable for
anti-SMURF1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Western Blot (WB) of
anti-SMURF1 antibody
Western Blot
Positive WB detected in:Mouse liver tissue,Mouse brain tissue
All lanes: SMURF1 antibody at 3.4ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 87,84 kDa
Observed band size: 87,70 kDa
Immunohistochemistry (IHC) of
anti-SMURF1 antibody
Immunohistochemistry of paraffin-embedded human kidney tissue using MBS7051296 at dilution of 1:100
Immunohistochemistry (IHC) of
anti-SMURF1 antibody
Immunohistochemistry of paraffin-embedded human testis tissue using MBS7051296 at dilution of 1:100
Immunofluorescence (IF) of
anti-SMURF1 antibody
Immunofluorescent analysis of Hela cells using MBS7051296 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescence (IF) of
anti-SMURF1 antibody
Immunofluorescent analysis of HepG2 cells cells using MBS7051296 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
NCBI/Uniprot data below describe general gene information for
SMURF1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001186776.1
[Other Products]
NCBI GenBank Nucleotide #
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[Other Products]
UniProt Primary Accession #
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[Other Products]
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
83,440 Da
NCBI Official Full Name
E3 ubiquitin-protein ligase SMURF1 isoform 3
NCBI Official Synonym Full Names
SMAD specific E3 ubiquitin protein ligase 1
NCBI Protein Information
E3 ubiquitin-protein ligase SMURF1
UniProt Protein Name
E3 ubiquitin-protein ligase SMURF1
UniProt Synonym Protein Names
HECT-type E3 ubiquitin transferase SMURF1; SMAD ubiquitination regulatory factor 1; SMAD-specific E3 ubiquitin-protein ligase 1
UniProt Synonym Gene Names
UniProt Entry Name
SMUF1_HUMAN
NCBI Summary for SMURF1
This gene encodes a ubiquitin ligase that is specific for receptor-regulated SMAD proteins in the bone morphogenetic protein (BMP) pathway. This protein plays a key roll in the regulation of cell motility, cell signalling, and cell polarity. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Dec 2010]
UniProt Comments for SMURF1
SMURF1: E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA. Interacts with TRAF4. Interacts (via HECT domain) with FBXL15 (via LRR repeats). Interacts with SMAD7 and TGFBR1; SMAD7 recruits SMURF1 to TGFBR1 and regulates TGF-beta receptor degradation. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 6.3.2.-; EC 6.3.2.19; Ligase; Ubiquitin conjugating system; Ubiquitin ligase
Chromosomal Location of Human Ortholog: 7q22.1
Cellular Component: cytoplasm; cytosol; mitochondrion; nucleoplasm; nucleus; plasma membrane
Molecular Function: phospholipid binding; protein binding; ubiquitin-protein ligase activity
Biological Process: BMP signaling pathway; cell differentiation; ectoderm development; negative regulation of BMP signaling pathway; negative regulation of transforming growth factor beta receptor signaling pathway; proteasomal ubiquitin-dependent protein catabolic process; protein export from nucleus; protein polyubiquitination; protein ubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process; receptor catabolic process; transforming growth factor beta receptor signaling pathway; ubiquitin-dependent SMAD protein catabolic process; Wnt receptor signaling pathway, planar cell polarity pathway
Research Articles on SMURF1
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice. It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated with anti-SMURF1 antibody
Pathways associated with anti-SMURF1 antibody
Diseases associated with anti-SMURF1 antibody
Organs/Tissues associated with anti-SMURF1 antibody
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