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anti-SPAM1 antibody :: Rabbit anti-Human SPAM1 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS2520659
Unit / Price
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 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

SPAM1, Polyclonal Antibody

★Popular Item★
 Also Known As   

SPAM1 Antibody

 Product Synonym Names    HYA1; PH20; HYAL1; HYAL3; HYAL5; PH-20; SPAG15
 Product Gene Name   

anti-SPAM1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    600930
 3D Structure    ModBase 3D Structure for P38567
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human
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 Purity/Purification    Affinity purification
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 Immunogen    Recombinant protein of human SPAM1
 Buffer    PBS with 0.02% sodium azide, 50% glycerol, pH7.3
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 Preparation and Storage    Store at -20C. Avoid freeze / thaw cycles.
 Other Notes    Small volumes of anti-SPAM1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-SPAM1 antibody

   Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21. 3 and 7q31. 3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1; another family member on chromosome 3p21. 3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]
 Applications Tested/Suitable for anti-SPAM1 antibody   

Western Blot (WB), Immunofluorescence (IF)

 Application Notes for anti-SPAM1 antibody    WB: 1:500-1:2000
IF: 1:50-1:200
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 Western Blot (WB) of anti-SPAM1 antibody    Western blot analysis of extracts of various cell lines, using SPAM1 antibody.
anti-SPAM1 antibody Western Blot (WB) (WB) image
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 Immunofluorescence (IF) of anti-SPAM1 antibody    Immunofluorescence analysis of HeLa cell using SPAM1 antibody. Blue: DAPI for nuclear staining.
anti-SPAM1 antibody Immunofluorescence (IF) image
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NCBI/Uniprot data below describe general gene information for SPAM1. It may not necessarily be applicable to this product.
 NCBI GI #    1480101
 NCBI GeneID    6677
 NCBI Accession #    CAA59086.1 [Other Products]
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 UniProt Primary Accession #    P38567 [Other Products]
 UniProt Secondary Accession #    Q8TC30 [Other Products]
 UniProt Related Accession #    P38567 [Other Products]
 Molecular Weight    58kDa
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 NCBI Official Full Name    sperm adhesion molecule gene SPAM1
 NCBI Official Synonym Full Names    sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding)
 NCBI Official Symbol    SPAM1 [Similar Products]
 NCBI Official Synonym Symbols   
HYA1; PH20; HYAL1; HYAL3; HYAL5; PH-20; SPAG15; HEL-S-96n
[Similar Products]
 NCBI Protein Information    hyaluronidase PH-20; epididymis secretory sperm binding protein Li 96n; hyal-PH20; hyaluronoglucosaminidase PH-20; sperm surface protein PH-20
 UniProt Protein Name    Hyaluronidase PH-20
 UniProt Synonym Protein Names   
Hyaluronoglucosaminidase PH-20; Sperm adhesion molecule 1; Sperm surface protein PH-20
 Protein Family    Hyaluronidase
 UniProt Gene Name    SPAM1 [Similar Products]
 UniProt Synonym Gene Names    HYAL3; PH20; Hyal-PH20 [Similar Products]
 UniProt Entry Name    HYALP_HUMAN
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 NCBI Summary for SPAM1    Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1; another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]
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 UniProt Comments for SPAM1    SPAM1: Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid. Belongs to the glycosyl hydrolase 56 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; Membrane protein, GPI anchor; EC 3.2.1.35; Cell surface; Glycan Metabolism - glycosaminoglycan degradation

Chromosomal Location of Human Ortholog: 7q31.3

Cellular Component: plasma membrane

Molecular Function: hyalurononglucosaminidase activity

Biological Process: sperm-egg recognition; fusion of sperm to egg plasma membrane; binding of sperm to zona pellucida; single fertilization; carbohydrate metabolic process; cell adhesion; multicellular organism reproduction
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 Research Articles on SPAM1    1. results indicate that neither rHuPH20 nor its directly generated HA catabolites have inflammatory properties in the air pouch model, and rHuPH20 can instead inhibit some aspects of inflammation, such as neutrophil infiltration into the air pouch.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-SPAM1 antibodyPathways associated with anti-SPAM1 antibody
 Reference Product  PubMed Publications
 HAS2 antibody  >2 publications with SPAM1 and HAS2
 Products by Pathway  Pathway Diagram
 Chondroitin Sulfate Degradation Pathway antibodies  Chondroitin Sulfate Degradation Pathway Diagram
 Chondroitin Sulfate Degradation Pathway antibodies  Chondroitin Sulfate Degradation Pathway Diagram
 Dermatan Sulfate Degradation Pathway antibodies  Dermatan Sulfate Degradation Pathway Diagram
 Dermatan Sulfate Degradation Pathway antibodies  Dermatan Sulfate Degradation Pathway Diagram
 Fertilization Pathway antibodies  Fertilization Pathway Diagram
 Glycosaminoglycan Degradation Pathway antibodies  Glycosaminoglycan Degradation Pathway Diagram
 Glycosaminoglycan Degradation Pathway antibodies  Glycosaminoglycan Degradation Pathway Diagram
 Interaction With Cumulus Cells Pathway antibodies  Interaction With Cumulus Cells Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Reproduction Pathway antibodies  Reproduction Pathway Diagram
Diseases associated with anti-SPAM1 antibodyOrgans/Tissues associated with anti-SPAM1 antibody
 Disease Name  Pubmed Publications
 Liver Neoplasms Antibodies  >1 publications with SPAM1 and Liver Neoplasms
 Nervous System Diseases Antibodies  >1 publications with SPAM1 and Nervous System Diseases
 Fetal Death Antibodies  >1 publications with SPAM1 and Fetal Death
 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >26 publications with SPAM1 and Testis
 Brain Antibodies  >1 publications with SPAM1 and Brain
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