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anti-SPHK1 antibody :: Goat SPHK1 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS421620
Western Blot (WB)
Unit / Price
0.1 mg  /  $300 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

SPHK1, Polyclonal Antibody

 Also Known As   

Goat anti-SPHK1 Antibody

 Product Synonym Names    Goat Anti-SPHK1 (C-term. ) Antibody; SPHK1; sphingosine kinase 1; SPHK; SK1; SKase; SPHK1 antibody; sphingosine kinase 1 antibody; SPHK antibody; SK1 antibody; SKase antibody
 Product Gene Name   

anti-SPHK1 antibody

[Similar Products]
 Antibody/Peptide Pairs    SPHK1 peptide (MBS426444) is used for blocking the activity of SPHK1 antibody (MBS421620)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence    DVDLESEKYR RLGE
 OMIM    603730
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 Clonality    Polyclonal
 Host    Goat
 Species Reactivity    Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
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 Purity/Purification    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
 Form/Format    Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
 Concentration    100ug specific antibody in 200ul (lot specific)
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 Immunogen    Peptide with sequence C-DVDLESEKYRRLGE, from the internal region of the protein sequence according to NP_068807.2; NP_892010.2; NP_001136073.1.
 Epitope    Internal region
 Note    This antibody is expected to recognise all three reported isoforms (NP_068807.2; NP_892010.2; NP_001136073.1). Reported variants represent identical protein (NP_001136073.1; NP_001136074.1).
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 Preparation and Storage    Aliquot and store at -20 degree C. Minimize freezing and thawing.
 ISO Certification    Manufactured in an ISO 9001:2015 Certified Laboratory.
 Other Notes    Small volumes of anti-SPHK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-SPHK1 antibody   

Peptide ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP)

 Application Notes for anti-SPHK1 antibody    Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: approx. 40 kDa band observed in Human Peripheral Blood Mononucleocyte lysates (calculated MW of 43.9 kDa according to NP_068807.2). Recommended concentration: 2-3ug/ml.
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 Western Blot (WB) of anti-SPHK1 antibody    (2ug/ml) staining of Human Peripheral Blood Mononucleocyte lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-SPHK1 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for SPHK1. It may not necessarily be applicable to this product.
 NCBI GI #    21361088
 NCBI GeneID    8877
 NCBI Accession #    NP_068807.2 [Other Products]
 NCBI Related Accession #    NP_892010.2; NP_001136073.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_021972.3 [Other Products]
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 UniProt Secondary Accession #    Q8N632; Q96GK1; Q9HD92; Q9NY70; Q9NYL3 [Other Products]
 UniProt Related Accession #    Q9NYA1 [Other Products]
 Molecular Weight    43,944 Da
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 NCBI Official Full Name    sphingosine kinase 1 isoform 1
 NCBI Official Synonym Full Names    sphingosine kinase 1
 NCBI Official Symbol    SPHK1 [Similar Products]
 NCBI Official Synonym Symbols   
SPHK
[Similar Products]
 NCBI Protein Information    sphingosine kinase 1
 UniProt Protein Name    Sphingosine kinase 1
 UniProt Gene Name    SPHK1 [Similar Products]
 UniProt Synonym Gene Names    SPHK; SPK; SK 1; SPK 1 [Similar Products]
 UniProt Entry Name    SPHK1_HUMAN
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 NCBI Summary for SPHK1    The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
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 UniProt Comments for SPHK1    SPHK1: Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. Interacts with ACY1. Binds to calmodulin. Interacts with SPHKAP. Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, lipid; EC 2.7.1.91; Lipid Metabolism - sphingolipid

Chromosomal Location of Human Ortholog: 17q25.2

Cellular Component: axon; cytoplasm; cytosol; nucleus; plasma membrane; synaptic vesicle

Molecular Function: ATP binding; calmodulin binding; D-erythro-sphingosine kinase activity; DNA binding; magnesium ion binding; protein binding; protein phosphatase 2A binding; sphinganine kinase activity

Biological Process: activation of NF-kappaB transcription factor; blood vessel development; brain development; calcium-mediated signaling; cellular response to starvation; cyclooxygenase pathway; female pregnancy; inflammatory response; lipid phosphorylation; negative regulation of apoptosis; positive regulation of angiogenesis; positive regulation of cell growth; positive regulation of cell migration; positive regulation of fibroblast proliferation; positive regulation of mitotic cell cycle; positive regulation of neurotransmitter secretion; positive regulation of NF-kappaB import into nucleus; positive regulation of protein ubiquitination; positive regulation of smooth muscle contraction; protein folding; regulation of interleukin-1 beta production; response to amine stimulus; response to ATP; response to magnesium ion; response to progesterone stimulus; signal transduction; sphingoid catabolic process; sphingolipid biosynthetic process; sphingosine biosynthetic process; sphingosine metabolic process
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 Research Articles on SPHK1    1. High SPHK1 protein correlated with resistance to cisplatin (IC50) in an independent gastric cancer cell line panel and with survival of patients treated with chemotherapy prior to surgery but not in patients treated with surgery alone.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-SPHK1 antibodyDiseases associated with anti-SPHK1 antibody
 Products by Pathway  Pathway Diagram
 Association Of TriC/CCT With Target Proteins During Biosynthesis Pathway antibodies  Association Of TriC/CCT With Target Proteins During Biosynthesis Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Chaperonin-mediated Protein Folding Pathway antibodies  Chaperonin-mediated Protein Folding Pathway Diagram
 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram
 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram
 Fc-epsilon Receptor I Signaling In Mast Cells Pathway antibodies  Fc-epsilon Receptor I Signaling In Mast Cells Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >84 publications with SPHK1 and Inflammation
 Carcinoma Antibodies  >49 publications with SPHK1 and Carcinoma
 Disease Models, Animal Antibodies  >47 publications with SPHK1 and Disease Models, Animal
 Necrosis Antibodies  >46 publications with SPHK1 and Necrosis
 Fibrosis Antibodies  >32 publications with SPHK1 and Fibrosis
 Lung Diseases Antibodies  >29 publications with SPHK1 and Lung Diseases
 Breast Neoplasms Antibodies  >23 publications with SPHK1 and Breast Neoplasms
 Liver Diseases Antibodies  >20 publications with SPHK1 and Liver Diseases
 Leukemia Antibodies  >20 publications with SPHK1 and Leukemia
 Kidney Diseases Antibodies  >18 publications with SPHK1 and Kidney Diseases
Organs/Tissues associated with anti-SPHK1 antibody
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >100 publications with SPHK1 and Blood
 Vascular Antibodies  >67 publications with SPHK1 and Vascular
 Lung Antibodies  >43 publications with SPHK1 and Lung
 Brain Antibodies  >38 publications with SPHK1 and Brain
 Liver Antibodies  >35 publications with SPHK1 and Liver
 Kidney Antibodies  >34 publications with SPHK1 and Kidney
 Bone Antibodies  >32 publications with SPHK1 and Bone
 Heart Antibodies  >26 publications with SPHK1 and Heart
 Bone Marrow Antibodies  >21 publications with SPHK1 and Bone Marrow
 Prostate Antibodies  >18 publications with SPHK1 and Prostate
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