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anti-SQSTM1 antibody :: Rabbit SQSTM1 (p62) Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS9204361
Unit / Price
0.08 mL  /  $150 +1 FREE 8GB USB
0.4 mL  /  $340 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

SQSTM1 (p62), Polyclonal Antibody

★Popular Item★
 Also Known As   

SQSTM1 (p62) Antibody (C-term)

 Product Synonym Names    Sequestosome-1; EBI3-associated protein of 60 kDa; EBIAP; p60; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; Ubiquitin-binding protein p62; SQSTM1; ORCA; OSIL
 Product Gene Name   

anti-SQSTM1 antibody

[Similar Products]
 Antibody/Peptide Pairs    SQSTM1 (p62) peptide (MBS9224625) is used for blocking the activity of SQSTM1 (p62) antibody (MBS9204361)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    601530
 3D Structure    ModBase 3D Structure for Q13501
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 Clonality    Polyclonal
 Isotype    Rabbit IgG.
 Host    Rabbit
 Species Reactivity    Human,Mouse,Rat

Predicted: Rat
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 Specificity    This SQSTM1 (p62) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 317-346 amino acids from the C-terminal region of human SQSTM1 (p62).
 Purity/Purification    Purified Rabbit Polyclonal Antibody (Pab)
 Form/Format    Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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 Antigen Region    317-346
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 Preparation and Storage    Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
 Other Notes    Small volumes of anti-SQSTM1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-SQSTM1 antibody

   SQSTM1/p62 is an adapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. This protein may play a role in titin/TTN downstream signaling in muscle cells, and may also regulate signaling cascades through ubiquitination. This protein is involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels. SQSTM1/p62 also appears to play a role in macroautophagic removal of intracellular protein aggregates. Cellular depletion studies of SQSTM1/p62 have indicated a role for association with LC3 and aggregate proteins in order to facilitate normal formation of the autophagosome.
 Product Categories/Family for anti-SQSTM1 antibody    Cardiovascular; Metabolism; Signal Transduction; Phospho Antibodies
 Applications Tested/Suitable for anti-SQSTM1 antibody   

WB, IHC-P , IF, E

 Application Notes for anti-SQSTM1 antibody    WB~~1:1000-1:2000
IHC-P~~1:50~100
IF~~1:50~100
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 Testing Data TD of anti-SQSTM1 antibody    All lanes : Anti-SQSTM1 (p62) Antibody (C-term) at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: THP-1 whole cell lysate Lane 5: PANC-1 whole cell lysate Lane 6: A20 whole cell lysate Lysates/proteins at 20 g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
anti-SQSTM1 antibody Testing Data TD image
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 Testing Data TD of anti-SQSTM1 antibody    All lanes : Anti-SQSTM1 (p62) Antibody (C-term) at 1:1000-1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: THP-1 whole cell lysate Lane 5: A20 whole cell lysate Lysates/proteins at 20 g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
anti-SQSTM1 antibody Testing Data TD image
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 Testing Data TD of anti-SQSTM1 antibody    MBS9204361 staining SQSTM1 in Human prostate tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
anti-SQSTM1 antibody Testing Data TD image
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 Testing Data TD of anti-SQSTM1 antibody    Fluorescent image of U251 cells stained with SQSTM1 (p62) (C-term) antibody. U251 cells were treated with Chloroquine (50 ?M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9204361 SQSTM1 (p62) (C-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ?g/ml, 5 min). SQSTM1 (p62) immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
anti-SQSTM1 antibody Testing Data TD image
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 Testing Data TD of anti-SQSTM1 antibody    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
anti-SQSTM1 antibody Testing Data TD image
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 Testing Data TD of anti-SQSTM1 antibody    Immunofluorescence staining of Autophagy SQSTM1 (p62) Antibody (C-term) (MBS9204361) on Methanol-fixed and PFA fixed HeLa cells. Data courtesy of Dr. Eeva-Liisa Eskelinen, University of Helsinki,Finland.
anti-SQSTM1 antibody Testing Data TD image
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NCBI/Uniprot data below describe general gene information for SQSTM1. It may not necessarily be applicable to this product.
 NCBI GI #    214830438
 NCBI GeneID    8878
 NCBI Accession #    NP_001135770.1 [Other Products]
 NCBI Related Accession #    NP_001135771.1; NP_003891.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001142298.1 [Other Products]
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 UniProt Primary Accession #    Q13501 [Other Products]
 UniProt Secondary Accession #    Q13446; Q9BUV7; Q9BVS6; Q9UEU1; A6NFN7; B2R661; B3KUW5 [Other Products]
 UniProt Related Accession #    Q13501 [Other Products]
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 NCBI Official Full Name    sequestosome-1 isoform 2
 NCBI Official Synonym Full Names    sequestosome 1
 NCBI Official Symbol    SQSTM1 [Similar Products]
 NCBI Official Synonym Symbols   
p60; p62; A170; OSIL; PDB3; ZIP3; p62B; FTDALS3
[Similar Products]
 NCBI Protein Information    sequestosome-1
 UniProt Protein Name    Sequestosome-1
 UniProt Synonym Protein Names   
EBI3-associated protein of 60 kDa; EBIAP; p60; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; Ubiquitin-binding protein p62
 Protein Family    Sequestosome
 UniProt Gene Name    SQSTM1 [Similar Products]
 UniProt Synonym Gene Names    ORCA; OSIL; EBIAP; p60 [Similar Products]
 UniProt Entry Name    SQSTM_HUMAN
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 NCBI Summary for SQSTM1    This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq, Mar 2009]
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 UniProt Comments for SQSTM1    SQSTM1: an adapter protein which binds ubiquitin, shuttling proteins to the proteasome. Co-localizes along with the E3 ubiquitin ligase, TRAF6, to aggregates from Alzheimer's disease brains but not in control brain. May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels. Forms ternary complexes with PKCZ and Kv-beta2 or PKCZ and GABBR3. Also interacts with KCNAB1, GABRR1, GABRR2 and GABRR3. Interacts with EBI3, LCK, RASA1, PKCZ , PKCI, NR2F2, NTRK1, NTRK2, NTRK3, NBR1, MEK5, TRIM55 and MAPKAPK5. Interacts with the proteasome subunits PSMD4 and PSMC2. Interacts with K63- polyubiquitinated MAPT/TAU. Interacts with IKBKB through PRKCZ and PRKCI. Interacts with NGFR through TRAF6 and bridges that complex to NTRK1. Forms a complex with MEK5 and PRKCZ or PRKCI. Component of a ternary complex with PAWR and PRKCZ. Upon TNF-alpha stimulation, interacts with RIPK1 problably bridging IKBKB to the TNF-R1 complex composed of TNF-R1/TNFRSF1A, TRADD and RIPK1. Forms a complex with JUB/Ajuba, PRKCZ and TRAF6. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Autophagy; Ubiquitin conjugating system; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 5q35

Cellular Component: nucleoplasm; PML body; lysosome; endoplasmic reticulum; cytoplasm; late endosome; autophagic vacuole; cytoplasmic vesicle; inclusion body; cytosol

Molecular Function: identical protein binding; protein serine/threonine kinase activity; ubiquitin binding; protein binding; protein homodimerization activity; protein kinase C binding; zinc ion binding; SH2 domain binding; receptor tyrosine kinase binding; protein kinase binding

Biological Process: regulation of Ras protein signal transduction; ubiquitin-dependent protein catabolic process; nerve growth factor receptor signaling pathway; immune system process; protein heterooligomerization; macroautophagy; positive regulation of apoptosis; endosome transport; protein amino acid phosphorylation; protein localization; response to stress; autophagy; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; regulation of I-kappaB kinase/NF-kappaB cascade; cell differentiation; positive regulation of macroautophagy; negative regulation of apoptosis

Disease: Frontotemporal Dementia And/or Amyotrophic Lateral Sclerosis 3; Paget Disease Of Bone
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Product References and Citations for anti-SQSTM1 antibody

   References for protein:
1.Seibenhener, M.L., et al., Mol. Cell. Biol. 24(18):8055-8068 (2004).
2.Eekhoff, E.W., et al., Arthritis Rheum. 50(5):1650-1654 (2004).
3.Brajenovic, M., et al., J. Biol. Chem. 279(13):12804-12811 (2004).
4.Kuusisto, E., et al., J. Neuropathol. Exp. Neurol. 62(12):1241-1253 (2003).
5. Johnson-Pais, T.L., et al., J. Bone Miner. Res. 18(10):1748-1753 (2003).
References for U251 cell line:
1. Westermark B.; Pontn J.; Hugosson R. (1973)." Determinants for the establishment of permanent tissue culture lines from human gliomas". Acta Pathol Microbiol Scand A. 81:791-805. [PMID: 4359449].
2. Pontn, J.,Westermark B. (1978)." Properties of Human Malignant Glioma Cells in Vitro". Medical Biology 56: 184-193.[PMID: 359950].
3. Geng Y.;Kohli L.; Klocke B.J.; Roth K.A.(2010). "Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent". Neuro Oncol. 12(5): 473-481.[ PMID: 20406898].
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 Research Articles on SQSTM1    1. SQSTM1 is required for proper dynein motility and trafficking along microtubules.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-SQSTM1 antibodyPathways associated with anti-SQSTM1 antibody
 Reference Product  PubMed Publications
 MAP1LC3B antibody  >53 publications with SQSTM1 and MAP1LC3B
 NBR1 antibody  >42 publications with SQSTM1 and NBR1
 KEAP1 antibody  >36 publications with SQSTM1 and KEAP1
 TRAF6 antibody  >26 publications with SQSTM1 and TRAF6
 MAP1LC3A antibody  >25 publications with SQSTM1 and MAP1LC3A
 WDFY3 antibody  >8 publications with SQSTM1 and WDFY3
 GABARAPL2 antibody  >8 publications with SQSTM1 and GABARAPL2
 PRKCZ antibody  >1 publications with SQSTM1 and PRKCZ
 Products by Pathway  Pathway Diagram
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Diseases associated with anti-SQSTM1 antibodyOrgans/Tissues associated with anti-SQSTM1 antibody
 Disease Name  Pubmed Publications
 Osteitis Deformans Antibodies  >103 publications with SQSTM1 and Osteitis Deformans
 Amyotrophic Lateral Sclerosis Antibodies  >59 publications with SQSTM1 and Amyotrophic Lateral Sclerosis
 Liver Diseases Antibodies  >54 publications with SQSTM1 and Liver Diseases
 Inflammation Antibodies  >53 publications with SQSTM1 and Inflammation
 Necrosis Antibodies  >51 publications with SQSTM1 and Necrosis
 Disease Models, Animal Antibodies  >48 publications with SQSTM1 and Disease Models, Animal
 Lung Diseases Antibodies  >31 publications with SQSTM1 and Lung Diseases
 Carcinoma, Hepatocellular Antibodies  >29 publications with SQSTM1 and Carcinoma, Hepatocellular
 Breast Neoplasms Antibodies  >25 publications with SQSTM1 and Breast Neoplasms
 Cardiovascular Diseases Antibodies  >25 publications with SQSTM1 and Cardiovascular Diseases
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >157 publications with SQSTM1 and Brain
 Bone Antibodies  >154 publications with SQSTM1 and Bone
 Liver Antibodies  >84 publications with SQSTM1 and Liver
 Blood Antibodies  >76 publications with SQSTM1 and Blood
 Muscle Antibodies  >75 publications with SQSTM1 and Muscle
 Nerve Antibodies  >59 publications with SQSTM1 and Nerve
 Lung Antibodies  >45 publications with SQSTM1 and Lung
 Heart Antibodies  >35 publications with SQSTM1 and Heart
 Kidney Antibodies  >30 publications with SQSTM1 and Kidney
 Vascular Antibodies  >21 publications with SQSTM1 and Vascular
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