|
| Product Name
|
|
SRY, Polyclonal Antibody ★Popular Item★
|
| Also Known As
|
|
Rabbit Anti-Bovine SRY
|
| Product Synonym Names
|
|
Anti-bovine SRY; Sex-determining region Y protein; Testis-determining factor
|
| Product Gene Name
|
|
|
[Similar Products]
|
| Research Use Only
|
|
For Research Use Only. Not for use in diagnostic procedures.
|
 TOP
|
|
| Chromosome Location
|
|
Chromosome: 13; AC_000170.1 (5424637..5425326, complement). Location: chromosome: Y
|
| 3D Structure
|
|
ModBase 3D Structure for Q03255
|
|
| Clonality
|
|
Polyclonal
|
| Clone Number
|
|
Rabbit IgG
|
| Host
|
|
Rabbit
|
| Species Reactivity
|
|
Bovine
|
|
|
| Purity/Purification
|
|
Protein A purified
|
| Form/Format
|
|
Lyophilized
|
|
|
| Immunogen
|
|
Recombinant bovine SRY (RT #B30-001)
|
| Preparation
|
|
Produced from sera of rabbits pre-immunized with highly pure recombinant bovine SRY (Met1 - Tyr229) derived from E. coli.
|
| Reconstitution
|
|
Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
|
| Buffer
|
|
PBS
|
|
|
| Preparation and Storage
|
|
The lyophilized antibody is stable at room temperature for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8 degree C. Frozen aliquots are stable for at least 6 months when stored at -20 degree C.
|
| ISO Certification
|
|
Manufactured in an ISO 9001:2015 Certified Laboratory.
|
| Other Notes
|
|
Small volumes of anti-SRY antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
|
|
TOP
|
|
|
|
|
The "Sex determining region of Y-Gen" (SRY), known as SRY gene, is coding for a transkription factor (TDF for Testis-determining factor). It belongs to the protein family of DNA binding-proteins. The SRY gene is beside other genes used for the determination of the sex in human as well as other mammals. Most mammals have for this purpose a further gene, UBE1. In humans SRY is normally located on the short arm of the Y chromosome. Accordingly, people who possess this chromosome with the corresponding gene, normally have a male phenotype. It does not matter how many copies of the X chromosome are present, even people with a multiple X chromosome (Klinefelter\'s syndrome) have this. The protein encoded by the gene Testis-determining factor controls the development of male sex.
|
|
| Applications Tested/Suitable for anti-SRY antibody
|
|
Western Blot (WB)
|
| Application Notes for anti-SRY antibody
|
|
Western Blot:Use 1-5 ug/ml
|
|
|
| Testing Data of anti-SRY antibody
|
|
|
|
TOP
|
|
| NCBI/Uniprot data below describe general gene information for SRY. It may not necessarily be applicable to this product. |
| NCBI GI #
|
|
62177084
|
| NCBI GeneID
|
|
280931
|
| NCBI Accession #
|
|
NP_001014407.1
|
[Other Products]
|
| NCBI GenBank Nucleotide #
|
|
NM_001014385.1
|
[Other Products]
|
|
|
| UniProt Primary Accession #
|
|
Q03255
|
[Other Products]
|
| UniProt Secondary Accession #
|
|
A1XCJ6; A8WDV8
|
[Other Products]
|
| UniProt Related Accession #
|
|
Q03255
|
[Other Products]
|
| Molecular Weight
|
|
26,627 Da
|
|
TOP
|
| NCBI Official Full Name
|
|
sex-determining region Y protein
|
| NCBI Official Symbol
|
|
SRY
|
[Similar Products]
|
| NCBI Protein Information
|
|
sex-determining region Y protein; sex determining factor Y; testis-determining factor; sex-determining region of the Y-chromosome
|
| UniProt Protein Name
|
|
Sex-determining region Y protein
|
| UniProt Synonym Protein Names
|
|
Testis-determining factor
|
| Protein Family
|
|
SRY-related protein
|
| UniProt Gene Name
|
|
SRY
|
[Similar Products]
|
| UniProt Synonym Gene Names
|
|
TDF
|
[Similar Products]
|
| UniProt Entry Name
|
|
SRY_BOVIN
|
|
|
| UniProt Comments for SRY
|
|
Function: Transcriptional regulator that controls a genetic switch in male development. It is necessary and sufficient for initiating male sex determination by directing the development of supporting cell precursors (pre-Sertoli cells) as Sertoli rather than granulosa cells. In male adult brain involved in the maintenance of motor functions of dopaminergic neurons. Involved in different aspects of gene regulation including promoter activation or repression. Promotes DNA bending. SRY HMG box recognizes DNA by partial intercalation in the minor groove. Also involved in pre-mRNA splicing. Binds to the DNA consensus sequence 5'-[AT]AACAA[AT]-3'
By similarity.
Subunit structure: Interacts with CALM, EP300, HDAC3, KPNB1, KRAB-O/ZFP748, PARP1, SLC9A3R2 and WT1
By similarity.
Subcellular location: Nucleus speckle
By similarity. Cytoplasm
By similarity.
Post-translational modification: Phosphorylation on serine residues enhances its DNA-binding activity and stimulates transcription repression. Acetylation of Lys-130 contributes to its nuclear localization and enhances its interaction with KPNB1. Deacetylated by HDAC3. Poly-ADP-ribosylation reduces its DNA-binding activity
By similarity.
Sequence similarities: Belongs to the SRY family.Contains 1 HMG box DNA-binding domain.
|
|
TOP
|
|
| Research Articles on SRY
|
|
1. analysis of mtDNA control region sequences and SRY gene sequences indicated more contribution to the Yunnan cattle from B. indicus than from B. taurus
|
|
TOP
|
| Precautions
|
|
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
|
| Disclaimer
|
|
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
|
|
TOP
|
|
| Pathways associated with anti-SRY antibody | Diseases associated with anti-SRY antibody |
|
|
|
|
|
|
TOP
|
Antibody 
