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anti-SPTLC1 antibody :: Rabbit anti-Human, Mouse Serine Palmitoyltransferase Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS2032142 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
0.01 mg  /  $110 +1 FREE 8GB USB
0.02 mg  /  $130 +1 FREE 8GB USB
0.05 mg  /  $185 +1 FREE 8GB USB
0.1 mg  /  $230 +1 FREE 8GB USB
0.2 mg  /  $345 +1 FREE 8GB USB
1 mg  /  $825 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Serine Palmitoyltransferase (SPTLC1), Polyclonal Antibody

★Popular Item★
 Also Known As   

Serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1) Polyclonal Antibody

 Product Synonym Names    HSAN; HSAN1; HSN1; LBC1; LCB1; SPT1; SPTI; Hereditary Sensory Neuropathy, Type 1; Long chain base biosynthesis protein 1; Serine-palmitoyl-CoA transferase 1
 Product Gene Name   

anti-SPTLC1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 3D Structure    ModBase 3D Structure for O35704
 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human, Mouse
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 Specificity    The antibody is a rabbit polyclonal antibody raised against SPTLC1. It has been selected for its ability to recognize SPTLC1 in immunohistochemical staining and western blotting.
 Purity/Purification    Affinity Chromatography
 Form/Format    Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
 Concentration    200ug/ml (lot specific)
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 Immunoglobulin Type    IgG
 Conjugate    No Conjugate
 Organism Species    Mouse
 Fragment    SPTLC1 (Asp143~Leu473)
 Quality Control    Content: The quality control contains recombinant SPTLC1 (Asp143~Leu473) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
 Conjugated Antibody    The APC conjugated antibody version of this item is also available as catalog #MBS2079301
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 Preparation and Storage    Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
 Other Notes    Small volumes of anti-SPTLC1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Product Categories/Family for anti-SPTLC1 antibody    Knock-Out Validation Antibody
 Applications Tested/Suitable for anti-SPTLC1 antibody   

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)

 Application Notes for anti-SPTLC1 antibody    Western Blot: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
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 Western Blot (WB) Knockout Verification of anti-SPTLC1 antibody    Knockout Verification: Lane 1: Wild-type Jurkat cell lysate; Lane 2: SPTLC1 knockout Jurkat cell lysate; Predicted MW: 53kd Observed MW: 55kd Primary Ab: 1ug/ml Rabbit Anti-Mouse SPTLC1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
anti-SPTLC1 antibody Western Blot (WB) Knockout Verification (WB) image
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 Western Blot (WB) of anti-SPTLC1 antibody    Western Blot; Sample: Lane1: Mouse Liver Tissue; Lane2: Mouse Lung Tissue; Lane3: Mouse Brain Tissue; Lane4: Human Jurkat Cells.
anti-SPTLC1 antibody Western Blot (WB) (WB) image
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 Western Blot (WB) of anti-SPTLC1 antibody    Western Blot; Sample: Recombinant SPTLC1, Mouse.
anti-SPTLC1 antibody Western Blot (WB) (WB) image
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 Immunohistochemistry (IHC) of anti-SPTLC1 antibody    DAB staining on IHC-P; Samples: Mouse Liver Tissue.
anti-SPTLC1 antibody Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of anti-SPTLC1 antibody    DAB staining on IHC-P; Samples: Mouse Uterus Tissue
anti-SPTLC1 antibody Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of anti-SPTLC1 antibody    DAB staining on IHC-P; Samples: Mouse Kidney Tissue.
anti-SPTLC1 antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for SPTLC1. It may not necessarily be applicable to this product.
 NCBI GI #    29244577
 NCBI GeneID    268656
 NCBI Accession #    NP_033295.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_009269.2 [Other Products]
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 UniProt Primary Accession #    O35704 [Other Products]
 UniProt Secondary Accession #    O54813; Q8BH11 [Other Products]
 UniProt Related Accession #    O35704 [Other Products]
 Molecular Weight    52,535 Da
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 NCBI Official Full Name    serine palmitoyltransferase 1
 NCBI Official Synonym Full Names    serine palmitoyltransferase, long chain base subunit 1
 NCBI Official Symbol    Sptlc1 [Similar Products]
 NCBI Official Synonym Symbols   
Lcb1; C77762; AW552086; E030036H05
[Similar Products]
 NCBI Protein Information    serine palmitoyltransferase 1
 UniProt Protein Name    Serine palmitoyltransferase 1
 UniProt Synonym Protein Names   
Long chain base biosynthesis protein 1; LCB 1; Serine-palmitoyl-CoA transferase 1; SPT 1; SPT1
 Protein Family    Serine palmitoyltransferase
 UniProt Gene Name    Sptlc1 [Similar Products]
 UniProt Synonym Gene Names    Lcb1; LCB 1; SPT 1; SPT1 [Similar Products]
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 UniProt Comments for SPTLC1    SPTLC1: Serine palmitoyltransferase (SPT). The heterodimer formed with SPTLC2 or SPTLC3 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA. The SPTLC1- SPTLC2-SPTSSB complex shows a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SPTSSB isozyme displays an ability to use a broader range of acyl-CoAs, without apparent preference. Defects in SPTLC1 are the cause of hereditary sensory and autonomic neuropathy type 1A (HSAN1A). The hereditary sensory and autonomic neuropathies are a genetically and clinically heterogeneous group of disorders characterized by degeneration of dorsal root and autonomic ganglion cells, and by sensory and/or autonomic abnormalities. HSAN1A is an autosomal dominant axonal neuropathy with onset in the second or third decades. Initial symptoms are loss of pain, touch, heat, and cold sensation over the feet, followed by distal muscle wasting and weakness. Loss of pain sensation leads to chronic skin ulcers and distal amputations. Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.3.1.50; Lipid Metabolism - sphingolipid; Membrane protein, integral; Transferase

Chromosomal Location of Human Ortholog: 13|13 B1

Cellular Component: endoplasmic reticulum; integral component of membrane; membrane; serine C-palmitoyltransferase complex

Molecular Function: catalytic activity; protein binding; pyridoxal phosphate binding; serine C-palmitoyltransferase activity; transferase activity; transferase activity, transferring acyl groups

Biological Process: biosynthetic process; ceramide biosynthetic process; lipid metabolic process; positive regulation of lipophagy; sphinganine biosynthetic process; sphingolipid biosynthetic process; sphingolipid metabolic process; sphingomyelin biosynthetic process; sphingosine biosynthetic process
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 Research Articles on SPTLC1    1. mice with an adipocyte-specific deletion of Sptlc1 show that de novo sphingolipid biosynthesis is required for adipocyte cell viability and normal metabolic function and that reduced de novo sphingolipid biosynthesis within adipocytes is associated with adipocyte death, adipose tissue remodeling, and metabolic dysfunction
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-SPTLC1 antibodyPathways associated with anti-SPTLC1 antibody
 Reference Product  PubMed Publications
 Sptlc2 antibody  >30 publications with SPTLC1 and Sptlc2
 Sptlc3 antibody  >5 publications with SPTLC1 and Sptlc3
 Abca1 antibody  >2 publications with SPTLC1 and Abca1
 Pard3 antibody  >1 publications with SPTLC1 and Pard3
 Sptssb antibody  >1 publications with SPTLC1 and Sptssb
 Sptssa antibody  >1 publications with SPTLC1 and Sptssa
 Products by Pathway  Pathway Diagram
 Ceramide Biosynthesis Pathway antibodies  Ceramide Biosynthesis Pathway Diagram
 Ceramide Biosynthesis Pathway antibodies  Ceramide Biosynthesis Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Sphingolipid De Novo Biosynthesis Pathway antibodies  Sphingolipid De Novo Biosynthesis Pathway Diagram
 Sphingolipid Metabolism Pathway antibodies  Sphingolipid Metabolism Pathway Diagram
 Sphingolipid Metabolism Pathway antibodies  Sphingolipid Metabolism Pathway Diagram
 Sphingolipid Metabolism Pathway antibodies  Sphingolipid Metabolism Pathway Diagram
 Sphingolipid Signaling Pathway antibodies  Sphingolipid Signaling Pathway Diagram
Diseases associated with anti-SPTLC1 antibodyOrgans/Tissues associated with anti-SPTLC1 antibody
 Disease Name  Pubmed Publications
 Fatty Liver Antibodies  >6 publications with SPTLC1 and Fatty Liver
 Inflammation Antibodies  >6 publications with SPTLC1 and Inflammation
 Atherosclerosis Antibodies  >6 publications with SPTLC1 and Atherosclerosis
 Liver Diseases Antibodies  >4 publications with SPTLC1 and Liver Diseases
 Necrosis Antibodies  >4 publications with SPTLC1 and Necrosis
 Nerve Degeneration Antibodies  >3 publications with SPTLC1 and Nerve Degeneration
 Fibrosis Antibodies  >2 publications with SPTLC1 and Fibrosis
 Insulin Resistance Antibodies  >2 publications with SPTLC1 and Insulin Resistance
 Weight Loss Antibodies  >2 publications with SPTLC1 and Weight Loss
 Carcinoma, Hepatocellular Antibodies  >1 publications with SPTLC1 and Carcinoma, Hepatocellular
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >20 publications with SPTLC1 and Blood
 Skin Antibodies  >9 publications with SPTLC1 and Skin
 Brain Antibodies  >9 publications with SPTLC1 and Brain
 Dorsal Root Ganglion Antibodies  >5 publications with SPTLC1 and Dorsal Root Ganglion
 Kidney Antibodies  >4 publications with SPTLC1 and Kidney
 Spinal Cord Antibodies  >3 publications with SPTLC1 and Spinal Cord
 Bone Antibodies  >3 publications with SPTLC1 and Bone
 Embryonic Tissue Antibodies  >3 publications with SPTLC1 and Embryonic Tissue
 Heart Antibodies  >2 publications with SPTLC1 and Heart
 Lung Antibodies  >2 publications with SPTLC1 and Lung
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