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anti-SHC1 antibody :: Rabbit Shc Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS625918 anti-SHC1 antibody
Unit / Price
0.05 mg  /  $410 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Shc (SHC1), Polyclonal Antibody

★Popular Item★
 Also Known As   

Shc, phosphorylated (Tyr427) (SHC-transforming Protein 1, SHC-transforming Protein 3, SHC-transforming Protein A, Src Homology 2 Domain-containing-transforming Protein C1, SH2 Domain Protein C1, SHC1, SHCA)

 Product Synonym Names    Anti -Shc, phosphorylated (Tyr427) (SHC-transforming Protein 1, SHC-transforming Protein 3, SHC-transforming Protein A, Src Homology 2 Domain-containing-transforming Protein C1, SH2 Domain Protein C1, SHC1, SHCA)
 Product Gene Name   

anti-SHC1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 1; NC_000001.10 (154934774..154946959, complement). Location: 1q21
 OMIM    600560
 3D Structure    ModBase 3D Structure for P29353
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human, Mouse, Rat
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 Specificity    Recognizes human Shc1 when phosphorylated at (Tyr427). Species Cross-Reactivity: mouse, rat.
 Purity/Purification    Affinity Purified
Purified by immunoaffinity chromatography.
 Form/Format    Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
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 Immunogen    Synthetic phosphopeptide corresponding to human Shc1 around the phosphorylation site of tyrosine 427 (P-S-YP-V-N).
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 Preparation and Storage    May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
 Other Notes    Small volumes of anti-SHC1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-SHC1 antibody

   Shc possesses SH2 and PTB domains. It serves a scaffolding function in signaling for a variety of receptor tyrosine kinases. Shc exists in p46, p52 and p66 isoforms. These isoforms are produced by using alternative translation initiation sites or a differentially spliced message. In response to extracellular signals, the SH2 and PTB domains of Shc interact with the activated receptors, leading to phosphorylation of Shc on three different tyrosine residues: Tyr239, Tyr240 and Tyr317. Grb2/Sos binds to Shc phosphorylated at these sites, activating the Ras/Raf/MAPK pathway. Both Shc expression and its tyrosine phosphorylation play an essential and nonredundant role in thymic T cell development.
 Product Categories/Family for anti-SHC1 antibody    Antibodies; Abs to Adapter Proteins
 Applications Tested/Suitable for anti-SHC1 antibody   

Western Blot (WB), Immunohistochemistry (IHC)

 Application Notes for anti-SHC1 antibody    Suitable for use in Western Blot, and Immunohistochemistry.
Dilution: Western Blot:1:500-1:1,000
Immunohistochemistry:1:50-1:100
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NCBI/Uniprot data below describe general gene information for SHC1. It may not necessarily be applicable to this product.
 NCBI GI #    32261324
 NCBI GeneID    6464
 NCBI Accession #    NP_003020.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_003029.4 [Other Products]
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 UniProt Primary Accession #    P29353 [Other Products]
 UniProt Secondary Accession #    O15290; Q5T180; Q5T183; Q5T184; Q5T185; Q5T186; Q8N4K5; Q96CL1; B5BU19; D3DV78 [Other Products]
 UniProt Related Accession #    P29353 [Other Products]
 Molecular Weight    62,822 Da [Similar Products]
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 NCBI Official Full Name    SHC-transforming protein 1 isoform 2
 NCBI Official Synonym Full Names    SHC (Src homology 2 domain containing) transforming protein 1
 NCBI Official Symbol    SHC1 [Similar Products]
 NCBI Official Synonym Symbols   
SHC; SHCA
[Similar Products]
 NCBI Protein Information    SHC-transforming protein 1; SH2 domain protein C1; SHC-transforming protein 3; SHC-transforming protein A; SHC (Src homology 2 domain-containing) transforming protein 1
 UniProt Protein Name    SHC-transforming protein 1
 UniProt Synonym Protein Names   
SHC-transforming protein 3; SHC-transforming protein A; Src homology 2 domain-containing-transforming protein C1
 Protein Family    SHC-transforming protein
 UniProt Gene Name    SHC1 [Similar Products]
 UniProt Synonym Gene Names    SHC; SHCA; SH2 domain protein C1 [Similar Products]
 UniProt Entry Name    SHC1_HUMAN
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 NCBI Summary for SHC1    This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]
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 UniProt Comments for SHC1    SHC1: an adaptor protein containing a SH2 domain and a PID domain within a PH domain-like fold. Couples activated growth factor receptors to signaling pathways. Participates in a signaling cascade initiated by activated KIT and KITLG/SCF. Six human isoforms are produced by alternative promoter usage and alternative splicing. Isoforms p66, p52 and p46 (P29353-1, -2, and -3), produced by alternative initiation, variously regulate growth factor signaling, oncogenesis, intracellular oxidant levels, and apoptosis. Isoforms p46 and p52, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex, thus initiating the cytoplasmic proliferative Ras signaling cascade in various non-neuronal systems. Isoform p66 does not mediate Ras activation, but associates with mitochondria where it controls intracellular redox status, mitochondrial permeability, life span, and stress-induced apoptosis. p66 acts as a downstream target of the tumor suppressor p53 and is required for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. P66 deletion in mice decreases the incidence of aging-associated diseases, such as atherosclerosis, and significantly prolongs life span. Participates in signaling downstream of TIE2, the tyrosine kinase receptor for angiopoietin, and plays a role in the regulation of endothelial cell migration and sprouting angiogenesis. Interacts with tyrosine-phosphorylated CD3T, DDR2, LRP1, IRS4, SHP, FLT4, PDGFRB, TIE2, TrkA, -B and -C. Interacts with the NPXY motif of tyrosine-phosphorylated IGF1R and INSR in vitro via the PID domain. p66Shc is known to be activated by the mutant SOD1 associated with familial forms of amyotrophic lateral sclerosis (ALS), causing a decrease in the activity of Rac1 through a redox-sensitive regulation. In case of oxidative conditions, phosphorylation at S36 of isoform p66Shc, leads to mitochondrial accumulation p66 plays a role in mediating mitophagy and determining neuronal cell fate following acute oxygen glucose deprivation. Isoform p46 is localized to the mitochondria matrix. Targeting of isoform p46Shc to mitochondria is mediated by its first 32 amino acids, which behave as a bona fide mitochondrial targeting sequence. Isoform p52Shc and isoform p66Shc, that contain the same sequence but more internally located, display a different subcellular localization.

Protein type: Adaptor/scaffold; Mitochondrial; Motility/polarity/chemotaxis; Apoptosis

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: mitochondrial matrix; plasma membrane; cytosol

Molecular Function: insulin-like growth factor receptor binding; protein binding; ephrin receptor binding; protein-tyrosine kinase activity; neurotrophin TRKA receptor binding; phospholipid binding; transmembrane receptor protein tyrosine kinase adaptor protein activity; protein kinase binding; insulin receptor binding; epidermal growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; platelet activation; peptidyl-tyrosine phosphorylation; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; unfolded protein response; activation of MAPK activity; heart development; regulation of epidermal growth factor receptor activity; MAPKKK cascade; cell-cell adhesion; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; regulation of growth; positive regulation of cell proliferation; Ras protein signal transduction; actin cytoskeleton reorganization; insulin receptor signaling pathway; innate immune response; angiogenesis; blood coagulation; leukocyte migration; positive regulation of DNA replication
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 Research Articles on SHC1    1. Knock-down of p66(Shc) leads to a positive feedback upregulation of Nrf2 expression and accordingly, Nrf2 is found to be highly expressed in tumors with low p66(Shc) expression
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-SHC1 antibodyDiseases associated with anti-SHC1 antibody
 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 Activation Of Chaperone Genes By XBP1(S) Pathway antibodies  Activation Of Chaperone Genes By XBP1(S) Pathway Diagram
 Activation Of Chaperones By IRE1alpha Pathway antibodies  Activation Of Chaperones By IRE1alpha Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Alpha6-Beta4 Integrin Signaling Pathway antibodies  Alpha6-Beta4 Integrin Signaling Pathway Diagram
 Angiopoietin Receptor Tie2-mediated Signaling Pathway antibodies  Angiopoietin Receptor Tie2-mediated Signaling Pathway Diagram
 Antigen Activates B Cell Receptor Leading To Generation Of Second Messengers Pathway antibodies  Antigen Activates B Cell Receptor Leading To Generation Of Second Messengers Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >196 publications with SHC1 and Neoplasms
 Carcinoma Antibodies  >52 publications with SHC1 and Carcinoma
 Cardiovascular Diseases Antibodies  >48 publications with SHC1 and Cardiovascular Diseases
 Breast Neoplasms Antibodies  >46 publications with SHC1 and Breast Neoplasms
 Cell Transformation, Neoplastic Antibodies  >36 publications with SHC1 and Cell Transformation, Neoplastic
 Inflammation Antibodies  >32 publications with SHC1 and Inflammation
 Nervous System Diseases Antibodies  >27 publications with SHC1 and Nervous System Diseases
 Neoplasms, Experimental Antibodies  >26 publications with SHC1 and Neoplasms, Experimental
 Kidney Diseases Antibodies  >22 publications with SHC1 and Kidney Diseases
 Lung Neoplasms Antibodies  >17 publications with SHC1 and Lung Neoplasms
Organs/Tissues associated with anti-SHC1 antibody
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >147 publications with SHC1 and Blood
 Vascular Antibodies  >70 publications with SHC1 and Vascular
 Nerve Antibodies  >64 publications with SHC1 and Nerve
 Muscle Antibodies  >64 publications with SHC1 and Muscle
 Brain Antibodies  >54 publications with SHC1 and Brain
 Liver Antibodies  >51 publications with SHC1 and Liver
 Kidney Antibodies  >48 publications with SHC1 and Kidney
 Heart Antibodies  >38 publications with SHC1 and Heart
 Lung Antibodies  >34 publications with SHC1 and Lung
 Bone Antibodies  >26 publications with SHC1 and Bone
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