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| Product Name
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Smad2, Polyclonal Antibody
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| Also Known As
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Smad2, phosphorylated (Ser467) (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)
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| Product Synonym Names
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Anti -Smad2, phosphorylated (Ser467) (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma)
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| Product Gene Name
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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 TOP
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| Chromosome Location
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Chromosome: 18; NC_000018.9 (45359466..45457515, complement). Location: 18q21.1
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| OMIM
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601366
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| 3D Structure
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ModBase 3D Structure for Q15796
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| Clonality
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Polyclonal
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| Isotype
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IgG
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| Host
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Rabbit
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| Species Reactivity
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Human
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| Specificity
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Recognizes endogenous levels of Smad2 only when phosphorylated at serine 467.
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| Purity/Purification
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Affinity Purified
Purified by immunoaffinity chromatography.
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| Form/Format
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Supplied as a liquid in PBS, pH 7.4, 150 mM NaCl, 0.02% sodium azide, 50% glycerol.
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| Immunogen
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Synthesized phosphopeptide derived from human Smad2 around the phosphorylation site of serine 467 (C-S-S-M-SP).
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| Preparation and Storage
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For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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| Other Notes
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Small volumes of anti-SMAD2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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TOP
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Smad2 is a 58kD member of a family of proteins involved in cell proliferation, differentiation and development. The Smad family is divided into three subclasses: receptor-regulated Smads, activin/TGFb receptor-regulated (Smad2 and 3) or BMP receptor regulated (Smad1, 5 and 8); the common partner, (Smad4) that functions via its interaction to the various Smads; and the inhibitory Smads, (Smad6 and Smad7). Smad2 consists of two highly conserved domains, the N-terminal Mad homology (MH1) and the C-terminal Mad homology 2 (MH2) domains. The MH1 domain binds DNA and regulates nuclear import and transcription while the MH2 domain conserved among all the Smads regulates Smad2 oligomerization and binding to cytoplasmic adaptors and transcription factors. Activated Smad2 associates with Smad4 and translocates as a complex into the nucleus, allowing its binding to DNA and transcription factors. This translocation of Smad2 (as well as Smad3) into the nucleus is a central event in TGFb signaling. Phosphorylation of threonine 8 in the calmodulin-binding region of the MH1 domain by extracellular signal-regulated kinase 1 (ERK1) enhances Smad2 transcriptional activity, which is negatively regulated by calmodulin. The regulation of Smad2 phosphorylation on threonine 8 by ERK1 and calmodulin is critical for Smad2-mediated signaling.
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| Product Categories/Family for anti-SMAD2 antibody
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Antibodies; Abs to Smad Proteins
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| Applications Tested/Suitable for anti-SMAD2 antibody
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Western Blot (WB)
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| Application Notes for anti-SMAD2 antibody
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Suitable for use in Western Blot.
Dilution: Western Blot: 1:500-1:1,000
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TOP
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| NCBI/Uniprot data below describe general gene information for SMAD2. It may not necessarily be applicable to this product. |
| NCBI GI #
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2967646
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| NCBI GeneID
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4087
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| UniProt Primary Accession #
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Q15796
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[Other Products]
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| UniProt Related Accession #
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Q15796; Q53XR6; Q7Z5E4
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[Other Products]
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| Molecular Weight
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52,306 Da
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[Similar Products]
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TOP
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| NCBI Official Full Name
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Smad2
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| NCBI Official Synonym Full Names
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SMAD family member 2
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| NCBI Official Symbol
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SMAD2
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[Similar Products]
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| NCBI Official Synonym Symbols
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JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2; MGC22139; MGC34440
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[Similar Products]
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| NCBI Protein Information
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mothers against decapentaplegic homolog 2; SMAD 2; MAD homolog 2; OTTHUMP00000163489; Mad protein homolog; Mad-related protein 2; mother against DPP homolog 2; mothers against DPP homolog 2; Sma- and Mad-related protein 2; SMAD, mothers against DPP homolog 2; MAD, mothers against decapentaplegic homolog 2; Mad, mothers against decapentaplegic homolog 2
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| UniProt Protein Name
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Mothers against decapentaplegic homolog 2
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| UniProt Synonym Protein Names
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JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2
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| Protein Family
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Mothers against decapentaplegic
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| UniProt Gene Name
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SMAD2
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[Similar Products]
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| UniProt Synonym Gene Names
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MADH2; MADR2
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[Similar Products]
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| UniProt Entry Name
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SMAD2_HUMAN
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| NCBI Summary for SMAD2
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The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq]
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| UniProt Comments for SMAD2
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SMAD2: ubiquitously expressed transcription factor phosphorylated and activated by TGF-beta receptor-type kinases. Participates in a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation and apoptosis. Phosphorylated Smads dimerize with collaborating Smad4 and are translocated into the nucleus, where the transcription of target genes is stimulated. Two alternatively spliced isoforms have been described.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 18q21.1
Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nuclear chromatin; cytosol; nucleus
Molecular Function: transcription activator binding; metal ion binding; transcription factor binding; phosphatase binding; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity; protein binding; DNA binding; ubiquitin protein ligase binding; double-stranded DNA binding; chromatin binding; SMAD binding; transcription factor activity; transforming growth factor beta receptor binding
Biological Process: developmental growth; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; activin receptor signaling pathway; paraxial mesoderm morphogenesis; gastrulation; negative regulation of transcription from RNA polymerase II promoter; primary microRNA processing; palate development; post-embryonic development; anterior/posterior pattern formation; negative regulation of cell proliferation; regulation of transforming growth factor beta receptor signaling pathway; ureteric bud development; transforming growth factor beta receptor signaling pathway; pancreas development; embryonic foregut morphogenesis; response to glucose stimulus; positive regulation of BMP signaling pathway; pericardium development; transcription initiation from RNA polymerase II promoter; cell fate commitment; regulation of binding; transcription, DNA-dependent; in utero embryonic development; common-partner SMAD protein phosphorylation; embryonic cranial skeleton morphogenesis; SMAD protein complex assembly; organ growth; zygotic determination of dorsal/ventral axis; mesoderm formation; insulin secretion; endoderm formation; gene expression; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transforming growth factor beta receptor signaling pathway; negative regulation of transcription, DNA-dependent; lung development
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TOP
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| Research Articles on SMAD2
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1. Smad2 is rapidly phosphorylated by treatment with lipoic acid within 30 min, enhancing type I collagen synthesis through the activation of Smad signaling.
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TOP
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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| Products associated with anti-SMAD2 antibody | Pathways associated with anti-SMAD2 antibody |
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| Diseases associated with anti-SMAD2 antibody | Organs/Tissues associated with anti-SMAD2 antibody |
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Antibody 
