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anti-Smad3 antibody :: Rabbit Smad3 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS858478
Unit / Price
0.1 mg  /  $215 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Smad3, Polyclonal Antibody

★Popular Item★
 Also Known As   

Smad3 (Phospho-Thr179) Antibody

 Product Synonym Names    JV15-2; MAD-3; MADH3; Mad3; Mothers against DPP homolog 3; Mothers against decapentaplegic homolog 3; SMAD 3; Smad 3
 Product Gene Name   

anti-Smad3 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    gene 613795
 3D Structure    ModBase 3D Structure for P84022
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human, Mouse, Rat
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 Specificity    Smad3 (Phospho-Thr179) antibody detects endogenous levels of Smad3 only when phosphorylated at threonine 179.
 Purity/Purification    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation
 Form/Format    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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 Immunogen    The antiserum was produced against synthesized phosphopeptide derived from human Smad3 around the phosphorylation site of threonine 179 (P-E-TP-P-P).
 All Sites    Human: Thr179; Mouse: Thr179; Rat: Thr179
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 Preparation and Storage    Store at -20 degree C for 1 year
 Other Notes    Small volumes of anti-Smad3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Product Categories/Family for anti-Smad3 antibody    Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody
 Applications Tested/Suitable for anti-Smad3 antibody   

Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)

 Application Notes for anti-Smad3 antibody    WB: 1:500~1:1000, IHC: 1:50~1:100, ELISA: 1:1000
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 Testing Data of anti-Smad3 antibody    Immunohistochemistry analysis of paraffin-embedded human thymus gland tissue using Smad3 (Phospho-Thr179) antibody.
anti-Smad3 antibody Testing Data image
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 Testing Data #2 of anti-Smad3 antibody    Western blot analysis of extracts from HeLa cells, treated with TNF (20ng/ml, 2mins), using Smad3 (Phospho-Thr179) antibody.
anti-Smad3 antibody Testing Data #2 image
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NCBI/Uniprot data below describe general gene information for Smad3. It may not necessarily be applicable to this product.
 NCBI GI #    223029440
 NCBI GeneID    4088
 NCBI Accession #    NP_001138574.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001145102.1 [Other Products]
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 UniProt Primary Accession #    P84022 [Other Products]
 UniProt Secondary Accession #    O09064; O09144; O14510; O35273; Q92940; Q93002; A8K4B6; B7Z4Z5; B7Z6M9; B7Z9Q2; F5H383 [Other Products]
 UniProt Related Accession #    P84022 [Other Products]
 Molecular Weight    25,722 Da
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 NCBI Official Full Name    mothers against decapentaplegic homolog 3 isoform 2
 NCBI Official Synonym Full Names    SMAD family member 3
 NCBI Official Symbol    SMAD3 [Similar Products]
 NCBI Official Synonym Symbols   
LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
[Similar Products]
 NCBI Protein Information    mothers against decapentaplegic homolog 3; mad3; hMAD-3; hSMAD3; MAD homolog 3; mad homolog JV15-2; mad protein homolog; mothers against DPP homolog 3; SMA- and MAD-related protein 3; SMAD, mothers against DPP homolog 3; MAD, mothers against decapentaplegic homolog 3
 UniProt Protein Name    Mothers against decapentaplegic homolog 3
 UniProt Synonym Protein Names   
JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3
 Protein Family    Mothers against decapentaplegic
 UniProt Gene Name    SMAD3 [Similar Products]
 UniProt Synonym Gene Names    MADH3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; SMAD 3; Smad3; hSMAD3 [Similar Products]
 UniProt Entry Name    SMAD3_HUMAN
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 NCBI Summary for Smad3    The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009]
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 UniProt Comments for Smad3    SMAD3: transcription factor phosphorylated and activated by TGF-beta-type receptors. A receptor-regulated Smad (R-smad). Binds directly to consensus DNA-binding elements in the promoters of target genes. In mouse required for establishemnt of the mucosal immune response and proper development of skeleton.

Protein type: Nuclear receptor co-regulator; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 15q22.33

Cellular Component: nucleoplasm; transcription factor complex; nuclear chromatin; cytoplasm; plasma membrane; nuclear inner membrane; cytosol; nucleus; receptor complex

Molecular Function: identical protein binding; ubiquitin binding; protein homodimerization activity; zinc ion binding; chromatin DNA binding; beta-catenin binding; protein kinase binding; transcription factor binding; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity; phosphatase binding; collagen binding; protein binding; sequence-specific DNA binding; ubiquitin protein ligase binding; double-stranded DNA binding; bHLH transcription factor binding; transcription factor activity; transforming growth factor beta receptor binding

Biological Process: developmental growth; positive regulation of positive chemotaxis; positive regulation of transcription, DNA-dependent; activin receptor signaling pathway; paraxial mesoderm morphogenesis; embryonic pattern specification; regulation of transforming growth factor beta receptor signaling pathway; transforming growth factor beta receptor signaling pathway; positive regulation of stress fiber formation; embryonic foregut morphogenesis; negative regulation of mitotic cell cycle; negative regulation of osteoblast differentiation; cell cycle arrest; regulation of striated muscle development; pericardium development; somitogenesis; transcription, DNA-dependent; embryonic cranial skeleton morphogenesis; osteoblast development; regulation of transcription from RNA polymerase II promoter; positive regulation of chondrocyte differentiation; mesoderm formation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of osteoblast proliferation; negative regulation of protein catabolic process; negative regulation of apoptosis; endoderm development; evasion of host defenses by virus; T cell activation; wound healing; primary microRNA processing; negative regulation of transcription from RNA polymerase II promoter; regulation of epithelial cell proliferation; negative regulation of protein amino acid phosphorylation; positive regulation of focal adhesion formation; transport; ureteric bud development; positive regulation of transforming growth factor-beta3 production; positive regulation of transcription factor import into nucleus; thyroid gland development; heart looping; caspase activation; transcription initiation from RNA polymerase II promoter; intercellular junction assembly and maintenance; regulation of transforming growth factor-beta2 production; regulation of immune response; regulation of binding; protein stabilization; in utero embryonic development; positive regulation of bone mineralization; liver development; SMAD protein complex assembly; immune system development; positive regulation of interleukin-1 beta production; negative regulation of inflammatory response; response to hypoxia; immune response; gene expression; negative regulation of cell growth; negative regulation of transforming growth factor beta receptor signaling pathway; positive regulation of cell migration

Disease: Loeys-dietz Syndrome 3
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Product References and Citations for anti-Smad3 antibody

   Zhang Y., Nature 383:168-172(1996).
Riggins G.J., Nat. Genet. 13:347-349(1996).
Arai T., Cancer Lett. 122:157-163(1998).
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 Research Articles on Smad3    1. Study is consistent with a key role for KCa3.1 renal proximal tubular cells in mediating the TGF-beta1 induction of MCP-1 expression in HK2 cells via Smad3, p38 and ERK1/2 MAPK signaling pathways.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-Smad3 antibodyDiseases associated with anti-Smad3 antibody
 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Alpha6-Beta4 Integrin Signaling Pathway antibodies  Alpha6-Beta4 Integrin Signaling Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Disease Name  Pubmed Publications
 Fibrosis Antibodies  >871 publications with Smad3 and Fibrosis
 Inflammation Antibodies  >329 publications with Smad3 and Inflammation
 Disease Models, Animal Antibodies  >316 publications with Smad3 and Disease Models, Animal
 Liver Diseases Antibodies  >223 publications with Smad3 and Liver Diseases
 Cardiovascular Diseases Antibodies  >199 publications with Smad3 and Cardiovascular Diseases
 Kidney Diseases Antibodies  >198 publications with Smad3 and Kidney Diseases
 Adenocarcinoma Antibodies  >153 publications with Smad3 and Adenocarcinoma
 Breast Neoplasms Antibodies  >124 publications with Smad3 and Breast Neoplasms
 Liver Cirrhosis Antibodies  >120 publications with Smad3 and Liver Cirrhosis
 Necrosis Antibodies  >116 publications with Smad3 and Necrosis
Organs/Tissues associated with anti-Smad3 antibody
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >572 publications with Smad3 and Muscle
 Blood Antibodies  >405 publications with Smad3 and Blood
 Bone Antibodies  >381 publications with Smad3 and Bone
 Kidney Antibodies  >369 publications with Smad3 and Kidney
 Lung Antibodies  >363 publications with Smad3 and Lung
 Liver Antibodies  >337 publications with Smad3 and Liver
 Vascular Antibodies  >284 publications with Smad3 and Vascular
 Connective Tissue Antibodies  >280 publications with Smad3 and Connective Tissue
 Skin Antibodies  >226 publications with Smad3 and Skin
 Heart Antibodies  >190 publications with Smad3 and Heart
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