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anti-SFTPA1 antibody :: Rabbit anti-Mouse, Rat Surfactant Protein A Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS175612
Unit / Price
0.1 mg  /  $245 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Surfactant Protein A (SFTPA1), Polyclonal Antibody

★Popular Item★
 Also Known As   

Polyclonal Anti-SFTPA1 Antibody

 Product Synonym Names    Pulmonary surfactant-associated protein A; surfactant protein A1
 Product Gene Name   

anti-SFTPA1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 3D Structure    ModBase 3D Structure for P35242
 Clonality    Polyclonal
 Isotype    Rabbit IgG
 Host    Rabbit
 Species Reactivity    Mouse, Rat
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 Specificity    No cross reactivity with other proteins.
 Purity/Purification    Immunogen affinity purified.
 Form/Format    Lyophilized
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 Immunogen    A synthetic peptide corresponding to a sequence at the C-terminus of mouse Surfactant Protein A (233-248aa WNDKGCLQYRLAICEF), identical to the related rat sequence.
 Contents    Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
 Reconstitution    0.2 ml of distilled water will yield a concentration of 500 ug/ml.
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 Preparation and Storage    At -20C for one year. After reconstitution, at 4C for one month. It can also be aliquotted and stored frozen at -20C for a longer time. Avoid repeated freezing and thawing.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of anti-SFTPA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-SFTPA1 antibody

   Description: Rabbit IgG polyclonal antibody for Pulmonary surfactant-associated protein A(SFTPA1) detection. Tested with WB, IHC-P in Mouse, Rat.
Background: Surfactant protein A is an innate immune system collectin. The gene encoding SP-A map to mouse chromosome 14. Surfactant protein-A, which plays a role in innate host defense in the lung, is also expressed in the Eustachian tube. We report that the frequency of specific surfactant protein-A haplotypes and genotypes differs between children with recurrent otitis media compared with a control population.
 Applications Tested/Suitable for anti-SFTPA1 antibody   

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

 Application Notes for anti-SFTPA1 antibody    Western Blot:
Concentration: 0.1-0.5ug/ml
Tested Species: Ms, Rat

Immunohistochemistry (Paraffin-emmbedded Section):
Concentration: 0.5-1ug/ml
Tested Species: Rat
Predicted Species: Ms
Antigen Retrieval: By Heat

Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determiend by end users.
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 Western Blot (WB) of anti-SFTPA1 antibody    Figure 1. Western blot analysis of SFTPA1 using anti- SFTPA1 antibody (MBS175612).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse lung tissue lysates,
Lane 2: mouse lung tissue lysates,
Lane 3: rat lung tissue lysates,
Lane 4: rat lung tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- SFTPA1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SFTPA1 at approximately 26KD. The expected band size for SFTPA1 is at 26KD.
anti-SFTPA1 antibody Western Blot (WB) (WB) image
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 Immunohistochemistry (IHC) of anti-SFTPA1 antibody    Figure 2. IHC analysis of SFTPA1 using anti- SFTPA1 antibody (MBS175612).
SFTPA1 was detected in paraffin-embedded section of rat lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- SFTPA1 Antibody (MBS175612) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
anti-SFTPA1 antibody Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of anti-SFTPA1 antibody    Figure 3. IHC analysis of SFTPA1 using anti- SFTPA1 antibody (MBS175612).
SFTPA1 was detected in paraffin-embedded section of mouse lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- SFTPA1 Antibody (MBS175612) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
anti-SFTPA1 antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for SFTPA1. It may not necessarily be applicable to this product.
 NCBI GI #    464483
 NCBI GeneID    20387
 NCBI Accession #    P35242.1 [Other Products]
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 UniProt Primary Accession #    P35242 [Other Products]
 UniProt Related Accession #    P35242 [Other Products]
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 NCBI Official Full Name    Pulmonary surfactant-associated protein A
 NCBI Official Synonym Full Names    surfactant associated protein A1
 NCBI Official Symbol    Sftpa1 [Similar Products]
 NCBI Official Synonym Symbols   
SP-A; Sftp1; Sftp-1
[Similar Products]
 NCBI Protein Information    pulmonary surfactant-associated protein A; PSAP; PSP-A; surfactant pulmonary associated protein A1
 UniProt Protein Name    Pulmonary surfactant-associated protein A
 Protein Family    Pulmonary surfactant-associated protein
 UniProt Gene Name    Sftpa1 [Similar Products]
 UniProt Synonym Gene Names    Sftp-1; Sftp1; Sftpa; PSAP; PSP-A; SP-A [Similar Products]
 UniProt Entry Name    SFTPA_MOUSE
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 UniProt Comments for SFTPA1    SFTPA2: In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration. Defects in SFTPA2 are a cause of pulmonary fibrosis idiopathic (IPF). Pulmonary fibrosis is a lung disease characterized by shortness of breath, radiographically evident diffuse pulmonary infiltrates, and varying degrees of inflammation and fibrosis on biopsy. It results in acute lung injury with subsequent scarring and endstage lung disease. Belongs to the SFTPA family.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: multivesicular body; extracellular space; rough endoplasmic reticulum; cytoplasmic vesicle

Biological Process: positive regulation of phagocytosis
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 Research Articles on SFTPA1    1. peptide from the interface with TLR4 inhibits endotoxic shock-like symptoms in a mouse model
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-SFTPA1 antibodyPathways associated with anti-SFTPA1 antibody
 Reference Product  PubMed Publications
 Sftpd antibody  >8 publications with SFTPA1 and Sftpd
 Sftpb antibody  >7 publications with SFTPA1 and Sftpb
 Sftpc antibody  >6 publications with SFTPA1 and Sftpc
 Scgb1a1 antibody  >1 publications with SFTPA1 and Scgb1a1
 Scgb3a2 antibody  >1 publications with SFTPA1 and Scgb3a2
 Products by Pathway  Pathway Diagram
 Pertussis Pathway antibodies  Pertussis Pathway Diagram
 Pertussis Pathway antibodies  Pertussis Pathway Diagram
 Phagosome Pathway antibodies  Phagosome Pathway Diagram
 Phagosome Pathway antibodies  Phagosome Pathway Diagram
Organs/Tissues associated with anti-SFTPA1 antibody
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >78 publications with SFTPA1 and Lung
 Embryonic Tissue Antibodies  >7 publications with SFTPA1 and Embryonic Tissue
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