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anti-SYT1 antibody :: Rabbit anti-Rat Synaptotagmin (Ser309) Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS502122
Testing Data
Unit / Price
0.1 mL  /  $360 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Synaptotagmin (Ser309) (SYT1), Polyclonal Antibody

 Also Known As   

Anti-Phospho-Ser309 Synaptotagmin

 Product Gene Name   

anti-SYT1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 7; NC_005106.3 (50097034..50289950, complement). Location: 7q21
 3D Structure    ModBase 3D Structure for P21707
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 Clonality    Polyclonal
 Host    Rabbit
 Species Reactivity    Rat
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 Specificity    Specific for the ~60k-62k synaptotagmin protein phosphorylated at Ser309.
 Purity/Purification    Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
 Form/Format    100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
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 Antigen    Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser309 of synaptotagmin.
 Immunogen Information    Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser309 conjugated to KLH
 Immunogen Species    Rat
 Reactivity Assumed Based on 100% Sequence Homology    Bovine, canine, chicken, human, mouse, non-human primate and zebra fish
 Species Reactivity Note    The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, human, mouse, non-human primate and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
 Biological Significance    Synaptotagmin is widely regarded as the primary calcium sensor for synaptic vesicle exocytosis (Fernandez-Chacon et al., 2001; Wang et al., 2003). Moreover, recent studies indicate that the protein also plays a key role in endocytosis (Poskanzer et al., 2003). Synaptotagmin can be phosphorylated by multiple protein kinases and this may play a key role in modulation of synaptotagmin's ability to influence both the exocytotic and endocytotic components of synaptic transmission (Hilfiker et al., 1999; Lee et al., 2004).
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 Preparation and Storage    For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.
 Other Notes    Small volumes of anti-SYT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-SYT1 antibody

   Affinity purified rabbit polyclonal antibody
 Applications Tested/Suitable for anti-SYT1 antibody   

Western Blot (WB), Immunohistochemistry (IHC)

 Application Notes for anti-SYT1 antibody    Quality Control: Western blots performed on each lot.
WB: 1:1000
IHC: 1:400
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 Testing Data of anti-SYT1 antibody    anti-SYT1 antibody Testing Data image
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NCBI/Uniprot data below describe general gene information for SYT1. It may not necessarily be applicable to this product.
 NCBI GI #    148356226
 NCBI GeneID    25716
 NCBI Accession #    NP_001028852.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_001033680.2 [Other Products]
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 UniProt Primary Accession #    P21707 [Other Products]
 UniProt Secondary Accession #    Q3S2E6; Q707P5 [Other Products]
 UniProt Related Accession #    P21707 [Other Products]
 Molecular Weight    60/62
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 NCBI Official Full Name    synaptotagmin-1
 NCBI Official Synonym Full Names    synaptotagmin I
 NCBI Official Symbol    Syt1 [Similar Products]
 NCBI Official Synonym Symbols   
P65
[Similar Products]
 NCBI Protein Information    synaptotagmin-1; sytI; synaptotagmin 1
 UniProt Protein Name    Synaptotagmin-1
 UniProt Synonym Protein Names   
Synaptotagmin I; SytI; p65
 Protein Family    Synaptotagmin
 UniProt Gene Name    Syt1 [Similar Products]
 UniProt Synonym Gene Names    SytI [Similar Products]
 UniProt Entry Name    SYT1_RAT
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 NCBI Summary for SYT1    integral component of synaptic vesicle membranes; important for exocytosis and vesicle trafficking to the active zone of synapses; may be the calcium sensor for calcium-dependent exocytosis [RGD, Feb 2006]
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 UniProt Comments for SYT1    Function: May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca2+-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca2+-independent manner; these are neurexins, syntaxin and AP2.

Cofactor: Binds 3 calcium ions per subunit. The ions are bound to the C2 domains.

Subunit structure: Homotetramer

Probable. Interacts with SCAMP5 and STON2. Forms a complex with SV2B, syntaxin 1 and SNAP25

By similarity. Interacts with RIMS1. Interacts with SV2A, SV2B and SV2C. Ref.5 Ref.6 Ref.8

Subcellular location: Cytoplasmic vesicle › secretory vesicle › synaptic vesicle membrane; Single-pass membrane protein. Cytoplasmic vesicle › secretory vesicle › chromaffin granule membrane; Single-pass membrane protein. Cytoplasm. Note: Synaptic vesicles and chromaffin granules.

Tissue specificity: Predominantly expressed in rostral, phylogenetically younger brain regions, and in some endocrine tissues.

Domain: The first C2 domain mediates Ca2+-dependent phospholipid binding. Ref.5The second C2 domain mediates interaction with SV2A and probably with STN2. Ref.5

Sequence similarities: Belongs to the synaptotagmin family.Contains 2 C2 domains.
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Product References and Citations for anti-SYT1 antibody

   • Fernandez-Chacon R, Konigstorfer A, Gerber SH, Garcia J, Matos MF, Stevens CF, Brose N, Rizo J, Rosenmund C, Sudhof TC (2001) Synaptotagmin I functions as a calcium regulator of release probability. Nature (London) 410:41-49.
• Hilfiker S, Pieribone VA, Nordstedt C, Greengard P, Czernik AJ (1999) Regulation of synaptotagmin I phosphorylation by multiple protein kinases. J Neurochem 73:921-932.
• Lee BH, Min X, Heise CJ, Xu BE, Chen S, Shu H, Luby-Phelps K, Goldsmith EJ, Cobb MH (2004) WNK1 phosphorylates synaptotagmin 2 and modulates its membrane binding. Mol Cell 15:741-751.
• Poskanzer KE, Marek KW, Sweeney ST, Davis GW (2003) Synaptotagmin I is necessary for compensatory synaptic vesicle endocytosis in vivo. Nature (London) 426:559-563.
• Wang CT, Lu JC, Bai JH, Chang PY, Martin TFJ, Chapman ER, Jackson MB (2003) Different domains of synaptotagmin control the choice between kiss-and-run and full fusion. Nature (London) 424:943-947.
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 Research Articles on SYT1    1. Analysis of SNARE complex/synaptotagmin-1 interactions by one-dimensional NMR Spectroscopy
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-SYT1 antibodyDiseases associated with anti-SYT1 antibody
 Products by Pathway  Pathway Diagram
 Acetylcholine Neurotransmitter Release Cycle Pathway antibodies  Acetylcholine Neurotransmitter Release Cycle Pathway Diagram
 GABA Synthesis, Release, Reuptake And Degradation Pathway antibodies  GABA Synthesis, Release, Reuptake And Degradation Pathway Diagram
 Glutamate Neurotransmitter Release Cycle Pathway antibodies  Glutamate Neurotransmitter Release Cycle Pathway Diagram
 Neuronal System Pathway antibodies  Neuronal System Pathway Diagram
 Neurotransmitter Release Cycle Pathway antibodies  Neurotransmitter Release Cycle Pathway Diagram
 Norepinephrine Neurotransmitter Release Cycle Pathway antibodies  Norepinephrine Neurotransmitter Release Cycle Pathway Diagram
 Synaptic Vesicle Cycle Pathway antibodies  Synaptic Vesicle Cycle Pathway Diagram
 Synaptic Vesicle Cycle Pathway antibodies  Synaptic Vesicle Cycle Pathway Diagram
 Transmission Across Chemical Synapses Pathway antibodies  Transmission Across Chemical Synapses Pathway Diagram
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >25 publications with SYT1 and Nervous System Diseases
 Brain Diseases Antibodies  >16 publications with SYT1 and Brain Diseases
 Neoplasms Antibodies  >12 publications with SYT1 and Neoplasms
 Disease Models, Animal Antibodies  >7 publications with SYT1 and Disease Models, Animal
 Inflammation Antibodies  >4 publications with SYT1 and Inflammation
 Seizures Antibodies  >4 publications with SYT1 and Seizures
 Movement Disorders Antibodies  >3 publications with SYT1 and Movement Disorders
 Learning Disorders Antibodies  >3 publications with SYT1 and Learning Disorders
 Poisoning Antibodies  >2 publications with SYT1 and Poisoning
 Pancreatic Neoplasms Antibodies  >2 publications with SYT1 and Pancreatic Neoplasms
Organs/Tissues associated with anti-SYT1 antibody
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >123 publications with SYT1 and Brain
 Eye Antibodies  >4 publications with SYT1 and Eye
 Dorsal Root Ganglion Antibodies  >3 publications with SYT1 and Dorsal Root Ganglion
 Pineal Gland Antibodies  >2 publications with SYT1 and Pineal Gland
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