anti-TMCO1 antibody :: Rabbit anti-Human TMCO1 Polyclonal Antibody
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
MBS7051543 Technical Datasheet
Purity/Purification
>95%, Protein G purified
Immunogen
Recombinant Human Calcium load-activated calcium channel protein (43-79AA)
Immunogen Species
Homo sapiens (Human)
Storage Buffer
Preservative: 0.03% Proclin300.
Constituents: 50% Glycerol, 0.01M PBS, pH7.4
Preparation and Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-TMCO1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for
anti-TMCO1 antibody
Calcium-selective channel required to prevent calcium stores from overfilling, thereby playing a key role in calcium homeostasis (PubMed:27212239). In response to endoplasmic reticulum overloading, assembles into a homotetramer, forming a functional calcium-selective channel, regulating the calcium content in endoplasmic reticulum store
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Applications Tested/Suitable for
anti-TMCO1 antibody
ELISA (EIA),Immunohistochemistry (IHC), Immunofluorescence (IF)
Western Blot (WB) of
anti-TMCO1 antibody
Western Blot
Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate, MCF-7 whole cell lysate, Mouse liver tissue, Mouse brain tissue,Rat heart tissue
All lanes: TMCO1 antibody at 3.4ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 28,17,12,22,18 KDa
Observed band size: 28 KDa
Immunohistochemistry (IHC) of
anti-TMCO1 antibody
IHC image of MBS7051543 diluted at 1:500 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunohistochemistry (IHC) of
anti-TMCO1 antibody
IHC image of MBS7051543 diluted at 1:500 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence (IF) of
anti-TMCO1 antibody
Immunofluorescent analysis of HepG2 cells using MBS7051543 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
NCBI/Uniprot data below describe general gene information for
TMCO1. It may not necessarily be applicable to this product.
UniProt Primary Accession #
Q9UM00
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Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice. It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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