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anti-TRIM21 antibody :: Rabbit TRIM21 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS9607373
Unit / Price
0.1 mL  /  $255 +1 FREE 8GB USB
0.2 mL  /  $295 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

TRIM21, Polyclonal Antibody

★Popular Item★
 Also Known As   

TRIM21 Antibody

 Product Synonym Names    52 kDa ribonucleoprotein autoantigen Ro/SS-A; 52 kDa Ro protein; 52kD Ro/SSA autoantigen; Autoantigen Ro/SSA, 52-KD; E3 ubiquitin-protein ligase TRIM21; RING finger protein 81; RNF81; Ro 52; Ro(SS-A); Ro52; RO52_HUMAN; Sicca syndrome antigen A; Sjoegren syndrome type A antigen; Sjogren syndrome antigen A1; Sjogren syndrome type A antigen; SS-A; SSA; SSA1: Sjogren syndrome antigen A1 (52kDa ribonucleoprotein autoantigen SS-A/Ro); TRIM21; Tripartite motif protein TRIM21; Tripartite motif-containing 21; Tripartite motif-containing protein 21
 Product Gene Name   

anti-TRIM21 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 3D Structure    ModBase 3D Structure for P19474
 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human, Mouse, Rat
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 Specificity    TRIM21 antibody detects endogenous levels of total TRIM21
 Purity/Purification    The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
 Form/Format    Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
 Concentration    1mg/ml (lot specific)
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 Immunogen    A synthesized peptide derived from human TRIM21
 Subcellular Location    Cytoplasm. Nucleus. Cytoplasm > P-Body. Enters the nucleus upon exposure to nitric oxide. Localizes to small dot-or rod-like structures in the cytoplasm, called cytoplasmic bodies (P-body) that are located underneath the plasma membrane and also diffusely in the cytoplasm and are highly motil in cells. Cytoplasmic bodies are located along the microtubules and do not share the same cytoplasmic bodies with TRIM5. Colocalizes with DCP2 in P-body.
 Tissue Specificity    Isoform 1 and isoform 2 are expressed in fetal and adult heart and fetal lung.
 Conjugation    Unconjugated
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 Preparation and Storage    Store at -20 degree C. Stable for 12 months from date of receipt.
 Other Notes    Small volumes of anti-TRIM21 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-TRIM21 antibody

   Description: TRIM21 has multiple N-terminal zinc finger motifs(has E3 ligase activity), a central leucine zipper, and a potential N-glycosylation site. TRIM3 is a E3 ubiquitin-protein ligase. It can form a ubiquitin ligase complex with E2 UBE2D2, which function not only for the uniquitination of USP4 and IKBKB but also for its self-ubiquitination. It has a role in the regulation of the cell cycle progression and could enhance the decapping activity of DCP2. It can exist in all mammalian cells as a ribonucleoprotein particle, which composed of a single polypeptide and 1 of 4 small RNA molecules.
Function: E3 ubiquitin-protein ligase whose activity is dependent on E2 enzymes, UBE2D1, UBE2D2, UBE2E1 and UBE2E2. Forms a ubiquitin ligase complex in cooperation with the E2 UBE2D2 that is used not only for the ubiquitination of USP4 and IKBKB but also for its self-ubiquitination. Component of cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes such as SCF(SKP2)-like complexes. A TRIM21-containing SCF(SKP2)-like complex is shown to mediate ubiquitination of CDKN1B ('Thr-187' phosphorylated-form), thereby promoting its degradation by the proteasome. Monoubiquitinates IKBKB that will negatively regulates Tax-induced NF-kappa-B signaling. Negatively regulates IFN-beta production post-pathogen recognition by polyubiquitin-mediated degradation of IRF3. Mediates the ubiquitin-mediated proteasomal degradation of IgG1 heavy chain, which is linked to the VCP-mediated ER-associated degradation (ERAD) pathway. Promotes IRF8 ubiquitination, which enhanced the ability of IRF8 to stimulate cytokine genes transcription in macrophages. Plays a role in the regulation of the cell cycle progression. Enhances the decapping activity of DCP2. Exists as a ribonucleoprotein particle present in all mammalian cells studied and composed of a single polypeptide and one of four small RNA molecules. At least two isoforms are present in nucleated and red blood cells, and tissue specific differences in RO/SSA proteins have been identified. The common feature of these proteins is their ability to bind HY RNAs.2. Involved in the regulation of innate immunity and the inflammatory response in response to IFNG/IFN-gamma. Organizes autophagic machinery by serving as a platform for the assembly of ULK1, Beclin 1/BECN1 and ATG8 family members and recognizes specific autophagy targets, thus coordinating target recognition with assembly of the autophagic apparatus and initiation of autophagy. Acts as an autophagy receptor for the degradation of IRF3, hence attenuating type I interferon (IFN)-dependent immune responses (PubMed:26347139).
Subunit Structure: Homotrimer (PubMed:17156811) (PubMed:26347139). Interacts (via C-terminus) with IRF8 (via C-terminus) (By similarity). Component of a SCF(SKP2)-like complex containing CUL1, SKP1, TRIM21 and SKP2. Interacts with CALR, CUL1, FBXW11, HSPA5, IKBKB, IRF3, SKP1 and VCP. Interacts with SKP2; the interaction with SKP2 does not depend on an intact F-box domain. Interacts (via N-terminus and C-terminus) with DCP2 (via N-terminus and C-terminus). Interacts with ULK1, BECN1 and with ATG8 family members, including GABARAP, GABARAPL1, GABARAPL2 and MAP1LC3C/LC3C. Interacts with TRIM21 and SQSTM1/sequestosome 1. Interacts with IRF3 (PubMed:26347139).
Post-translational Modifications: Autoubiquitinated; does not lead to its proteasomal degradation. Deubiquitinated by USP4; leading to its stabilization.
Similarity: The coiled-coil is necessary for the cytoplasmic localization. The B30.2/SPRY domain is necessary for the cytoplasmic localization, the interaction with IRF3 and for the IRF3-driven interferon beta promoter activity. The RING-type zinc finger is necessary for ubiquitination and for the IRF3-driven interferon beta promoter activity. Interacts with SKP2 and CUL1 in a RING finger-independent manner. Belongs to the TRIM/RBCC family.
 Applications Tested/Suitable for anti-TRIM21 antibody   

Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)

 Application Notes for anti-TRIM21 antibody    WB: 1:500-1:2000
IHC: 1:50-1:200
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 Western Blot (WB) of anti-TRIM21 antibody    Western blot analysis of extracts from mouse brain, using TRIM21 Antibody.
anti-TRIM21 antibody Western Blot (WB) (WB) image
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 Immunohistochemistry (IHC) of anti-TRIM21 antibody    MBS9607373 at 1/200 staining human brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
anti-TRIM21 antibody Immunohistochemistry (IHC) (IHC) image
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 Western Blot (WB) of anti-TRIM21 antibody    Western blot analysis of Hela whole cell lysates, using TRIM21 Antibody. The lane on the left is treated with the antigen-specific peptide.
anti-TRIM21 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for TRIM21. It may not necessarily be applicable to this product.
 NCBI GI #    15208660
 NCBI GeneID    6737
 NCBI Accession #    NP_003132.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_003141.3 [Other Products]
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 UniProt Primary Accession #    P19474 [Other Products]
 UniProt Secondary Accession #    Q5XPV5; Q96RF8 [Other Products]
 UniProt Related Accession #    P19474 [Other Products]
 Molecular Weight    Observed: 54 kDa
Predicted: 55 kDa
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 NCBI Official Full Name    E3 ubiquitin-protein ligase TRIM21
 NCBI Official Synonym Full Names    tripartite motif containing 21
 NCBI Official Symbol    TRIM21 [Similar Products]
 NCBI Official Synonym Symbols   
SSA; RO52; SSA1; RNF81; Ro/SSA
[Similar Products]
 NCBI Protein Information    E3 ubiquitin-protein ligase TRIM21
 UniProt Protein Name    E3 ubiquitin-protein ligase TRIM21
 UniProt Synonym Protein Names   
52 kDa Ro protein; 52 kDa ribonucleoprotein autoantigen Ro/SS-A; RING finger protein 81; RING-type E3 ubiquitin transferase TRIM21Curated; Ro(SS-A); Sjoegren syndrome type A antigen; SS-A; Tripartite motif-containing protein 21
 Protein Family    E3 ubiquitin-protein ligase
 UniProt Gene Name    TRIM21 [Similar Products]
 UniProt Synonym Gene Names    RNF81; RO52; SSA1; SS-A [Similar Products]
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 NCBI Summary for TRIM21    This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined. [provided by RefSeq, Jul 2008]
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 UniProt Comments for TRIM21    E3 ubiquitin-protein ligase whose activity is dependent on E2 enzymes, UBE2D1, UBE2D2, UBE2E1 and UBE2E2. Forms a ubiquitin ligase complex in cooperation with the E2 UBE2D2 that is used not only for the ubiquitination of USP4 and IKBKB but also for its self-ubiquitination. Component of cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes such as SCF(SKP2)-like complexes. A TRIM21-containing SCF(SKP2)-like complex is shown to mediate ubiquitination of CDKN1B ('Thr-187' phosphorylated-form), thereby promoting its degradation by the proteasome. Monoubiquitinates IKBKB that will negatively regulates Tax-induced NF-kappa-B signaling. Negatively regulates IFN-beta production post-pathogen recognition by polyubiquitin-mediated degradation of IRF3. Mediates the ubiquitin-mediated proteasomal degradation of IgG1 heavy chain, which is linked to the VCP-mediated ER-associated degradation (ERAD) pathway. Promotes IRF8 ubiquitination, which enhanced the ability of IRF8 to stimulate cytokine genes transcription in macrophages. Plays a role in the regulation of the cell cycle progression. Enhances the decapping activity of DCP2. Exists as a ribonucleoprotein particle present in all mammalian cells studied and composed of a single polypeptide and one of four small RNA molecules. At least two isoforms are present in nucleated and red blood cells, and tissue specific differences in RO/SSA proteins have been identified. The common feature of these proteins is their ability to bind HY RNAs.2. Involved in the regulation of innate immunity and the inflammatory response in response to IFNG/IFN-gamma. Organizes autophagic machinery by serving as a platform for the assembly of ULK1, Beclin 1/BECN1 and ATG8 family members and recognizes specific autophagy targets, thus coordinating target recognition with assembly of the autophagic apparatus and initiation of autophagy. Acts as an autophagy receptor for the degradation of IRF3, hence attenuating type I interferon (IFN)-dependent immune responses (PubMed:26347139).
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 Research Articles on TRIM21    1. Low TRIM21 expression is associated with RNA virus infections.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-TRIM21 antibodyPathways associated with anti-TRIM21 antibody
 Reference Product  PubMed Publications
 IRF7 antibody  >4 publications with TRIM21 and IRF7
 STAT1 antibody  >1 publications with TRIM21 and STAT1
 SSB antibody  >1 publications with TRIM21 and SSB
 TROVE2 antibody  >1 publications with TRIM21 and TROVE2
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Antigen Processing: Ubiquitination & Proteasome Degradation Pathway antibodies  Antigen Processing: Ubiquitination & Proteasome Degradation Pathway Diagram
 Class I MHC Mediated Antigen Processing & Presentation Pathway antibodies  Class I MHC Mediated Antigen Processing & Presentation Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Cytosolic Sensors Of Pathogen-associated DNA Pathway antibodies  Cytosolic Sensors Of Pathogen-associated DNA Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Interferon Signaling Pathway antibodies  Interferon Signaling Pathway Diagram
 Interferon Gamma Signaling Pathway antibodies  Interferon Gamma Signaling Pathway Diagram
 Regulation Of Innate Immune Responses To Cytosolic DNA Pathway antibodies  Regulation Of Innate Immune Responses To Cytosolic DNA Pathway Diagram
Diseases associated with anti-TRIM21 antibodyOrgans/Tissues associated with anti-TRIM21 antibody
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >8 publications with TRIM21 and Inflammation
 Disease Models, Animal Antibodies  >3 publications with TRIM21 and Disease Models, Animal
 Kidney Diseases Antibodies  >2 publications with TRIM21 and Kidney Diseases
 Nervous System Diseases Antibodies  >2 publications with TRIM21 and Nervous System Diseases
 Necrosis Antibodies  >2 publications with TRIM21 and Necrosis
 Cardiovascular Diseases Antibodies  >1 publications with TRIM21 and Cardiovascular Diseases
 Breast Neoplasms Antibodies  >1 publications with TRIM21 and Breast Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >17 publications with TRIM21 and Blood
 Lung Antibodies  >3 publications with TRIM21 and Lung
 Skin Antibodies  >2 publications with TRIM21 and Skin
 Heart Antibodies  >2 publications with TRIM21 and Heart
 Bone Antibodies  >1 publications with TRIM21 and Bone
 Bone Marrow Antibodies  >1 publications with TRIM21 and Bone Marrow
 Connective Tissue Antibodies  >1 publications with TRIM21 and Connective Tissue
 Mouth Antibodies  >1 publications with TRIM21 and Mouth
 Liver Antibodies  >1 publications with TRIM21 and Liver
 Kidney Antibodies  >1 publications with TRIM21 and Kidney
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