|
| Product Name
|
|
TRPA1, Polyclonal Antibody
|
| Also Known As
|
|
RABBIT ANTI HUMAN TRPA1
|
| Product Gene Name
|
|
|
[Similar Products]
|
| Research Use Only
|
|
For Research Use Only. Not for use in diagnostic procedures.
|
 TOP
|
|
| OMIM
|
|
604775
|
| 3D Structure
|
|
ModBase 3D Structure for O75762
|
|
| Clonality
|
|
Polyclonal
|
| Isotype
|
|
IgG
|
| Host
|
|
Rabbit
|
|
|
| Specificity
|
|
This item detects Transient receptor potential A1 (TRPA1), an excitatory cation channel that is highly expressed in nociceptive neurons and plays an important role in inflammatory pain. TRPA1 mediates the response to the proalgesic bradykinin as well as to pungent irritants found in mustards, garlic, and cinnamon.
TRPA1 may also be involved in hearing (as the mammalian hair cell mechanotransducer) and in the detection of cold stimuli.
|
| Form/Format
|
|
Purified
Purified IgG - liquid
|
| Concentration
|
|
IgG concentration 1.0mg/ml (lot specific)
|
|
|
| Perservative Stabilisers
|
|
0.1% Sodium Azide (NaN3)
|
| Immunogen
|
|
Synthetic peptide: internal domain of human TRPA1, conjugated to Keyhole Limpet Hemocyanin (KLH).
Histology Positive Control Tissue
|
| Antiserum Preparation
|
|
Antisera to human TRPA1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
|
| Target Species
|
|
Human
|
|
|
| Preparation and Storage
|
|
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
|
| Other Notes
|
|
Small volumes of anti-TRPA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
|
|
TOP
|
|
|
|
|
MBS222811 detects Transient receptor potential A1 (TRPA1), an excitatory cation channel that is highly expressed in nociceptive neurons and plays an important role in inflammatory pain. TRPA1 mediates the response to the proalgesic bradykinin as well as to pungent irritants found in mustards, garlic, and cinnamon. TRPA1 may also be involved in hearing (as the mammalian hair cell mechanotransducer) and in the detection of cold stimuli.
|
|
| Applications Tested/Suitable for anti-TRPA1 antibody
|
|
Immunohistology Paraffin*
|
| Application Notes for anti-TRPA1 antibody
|
|
Immunohistology - Paraffin: Minimum Dilution: 10; Maximum Dilution: 20ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
|
|
|
| Testing Data #1 of anti-TRPA1 antibody
|
|
Staining of human spinal cord doesal root gangion with Rabbit anti Human TRPA1 (MBS222811)
|
|
TOP
|
|
| NCBI/Uniprot data below describe general gene information for TRPA1. It may not necessarily be applicable to this product. |
| NCBI GI #
|
|
116534990
|
| NCBI GeneID
|
|
8989
|
| NCBI Accession #
|
|
NP_015628.2
|
[Other Products]
|
| NCBI GenBank Nucleotide #
|
|
NM_007332.2
|
[Other Products]
|
|
|
| UniProt Primary Accession #
|
|
O75762
|
[Other Products]
|
| UniProt Secondary Accession #
|
|
A6NIN6
|
[Other Products]
|
| UniProt Related Accession #
|
|
O75762
|
[Other Products]
|
| Molecular Weight
|
|
127,501 Da
|
|
TOP
|
| NCBI Official Full Name
|
|
transient receptor potential cation channel subfamily A member 1
|
| NCBI Official Synonym Full Names
|
|
transient receptor potential cation channel, subfamily A, member 1
|
| NCBI Official Symbol
|
|
TRPA1
|
[Similar Products]
|
| NCBI Official Synonym Symbols
|
|
FEPS; ANKTM1
|
[Similar Products]
|
| NCBI Protein Information
|
|
transient receptor potential cation channel subfamily A member 1; ankyrin-like with transmembrane domains 1; transformation-sensitive protein p120
|
| UniProt Protein Name
|
|
Transient receptor potential cation channel subfamily A member 1
|
| UniProt Synonym Protein Names
|
|
Ankyrin-like with transmembrane domains protein 1; Transformation-sensitive protein p120
|
| Protein Family
|
|
Transient receptor potential cation channel subfamily
|
| UniProt Gene Name
|
|
TRPA1
|
[Similar Products]
|
| UniProt Synonym Gene Names
|
|
ANKTM1
|
[Similar Products]
|
| UniProt Entry Name
|
|
TRPA1_HUMAN
|
|
|
| NCBI Summary for TRPA1
|
|
The structure of the protein encoded by this gene is highly related to both the protein ankyrin and transmembrane proteins. The specific function of this protein has not yet been determined; however, studies indicate the function may involve a role in signal transduction and growth control. [provided by RefSeq, Jul 2008]
|
|
|
| UniProt Comments for TRPA1
|
|
TRPA1: Receptor-activated non-selective cation channel involved in detection of pain and possibly also in cold perception and inner ear function. Has a central role in the pain response to endogenous inflammatory mediators and to a diverse array of volatile irritants, such as mustard oil, garlic and acrolein, an irritant from tears gas and vehicule exhaust fumes. Acts also as a ionotropic cannabinoid receptor by being activated by delta(9)- tetrahydrocannabinol (THC), the psychoactive component of marijuana. Not involved in menthol sensation. May be a component for the mechanosensitive transduction channel of hair cells in inner ear, thereby participating in the perception of sounds. Probably operated by a phosphatidylinositol second messenger system. Belongs to the transient receptor (TC 1.A.4) family.
Protein type: Membrane protein, multi-pass; Membrane protein, integral
Chromosomal Location of Human Ortholog: 8q13
Cellular Component: stereocilium bundle; integral to plasma membrane; plasma membrane
Molecular Function: channel activity; calcium channel activity
Biological Process: response to drug; detection of mechanical stimulus involved in sensory perception of pain; detection of chemical stimulus involved in sensory perception of pain; response to hydrogen peroxide; response to pain; ion transport; thermoception; response to cold; transmembrane transport
Disease: Episodic Pain Syndrome, Familial, 1
|
|
TOP
|
|
| Research Articles on TRPA1
|
|
1. analysis of the molecular basis determining inhibition and activation of nociceptive receptor TRPA1 protein
|
|
TOP
|
| Precautions
|
|
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
|
| Disclaimer
|
|
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
|
|
TOP
|
|
| Products associated with anti-TRPA1 antibody | Pathways associated with anti-TRPA1 antibody |
|
|
|
| Diseases associated with anti-TRPA1 antibody | Organs/Tissues associated with anti-TRPA1 antibody |
|
|
|
|
|
|
TOP
|
Antibody 
