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anti-S6K1 antibody :: Rabbit anti-Arabidopsis Thr449 S6 kinase 1 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS502218
Western Blot (WB)
Unit / Price
0.1 mL  /  $360 +1 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Thr449 S6 kinase 1 (S6K1), Polyclonal Antibody

★Popular Item★
 Also Known As   

Anti-Phospho-Thr449 S6 kinase 1 (S6K1)

 Product Synonym Names    S6 Kinase 1 (Thr449)
 Product Gene Name   

anti-S6K1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 3D Structure    ModBase 3D Structure for P42818
 Clonality    Polyclonal
 Host    Rabbit
 Species Reactivity    Arabidopsis
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 Specificity    Specific for the ~53k S6K1 protein phosphorylated at Thr449. The immunolabeling of the S6K1 band was completely blocked by the phosphopeptide used as antigen while the dephosphopeptide had no effect on the immunolabeling.
 Purity/Purification    Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
 Form/Format    100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. (Adequate amount of material to conduct 10 mini Western blots)
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 Immunogen Species    Arabidopsis
 Immunogen Info    Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr449 conjugated to KLH
 Biological Significance    Ribosomal s6 kinase is a member of a family of protein kinases involved in signal transduction. The subfamily S6K has two known homologues: S6K1 and S6K2. First characterized in mammals, S6K1 is controlled by target-of-rapamycin (TOR) kinase, which plays a central regulatory role in growth signaling pathways (Dufner and Thomas 1999). Osmotic stress inhibition of S6K is mediated by the TOR kinase pathway (Mahfouz et al., 2006). The activation of mammalian S6K1 involves phosphorylation at thr389 (Pearson et al., 2005), however its orthologue in Arabidopsis suggests that plant S6K1 thr449 is its functional equivalent (Schepetilnikov et al., 2011). The phytohormone auxin triggers TOR activation, which is followed by S6K1 phosphorylation at thr449, which in turn is critical for translation reinitiation (Schepetilnikov et al., 2013). Rapamycin effectively inactivates S6K1 thr449 phosphorylation in Arabidopsis seedlings, which suppresses TOR PK activity and ultimately plant growth (Xiong Y and Sheen J, 2011).
 Antigen    Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr449 of Arabidopsis S6K1.
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 Preparation and Storage    For long term storage -20C is recommended. Stable at -20C for at least 1 year.
 Other Notes    Small volumes of anti-S6K1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-S6K1 antibody

   Affinity purified rabbit polyclonal antibody
 Applications Tested/Suitable for anti-S6K1 antibody   

Western Blot (WB)

 Application Notes for anti-S6K1 antibody    WB: 1:1000
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 Western Blot (WB) of anti-S6K1 antibody    Western blot of Arabidopsis lysate showing specific immunolabeling of the ~53k S6K1 phosphorylated at Thr449. Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (P-peptide), but not by the corresponding nonphospho-peptide (NP-peptide).
anti-S6K1 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for S6K1. It may not necessarily be applicable to this product.
 NCBI GI #    15231960
 NCBI GeneID    820020
 NCBI Accession #    NP_187485.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_111707.3 [Other Products]
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 UniProt Primary Accession #    P42818 [Other Products]
 UniProt Secondary Accession #    Q8LFC1 [Other Products]
 UniProt Related Accession #    A54141 [Other Products]
 Molecular Weight    ~53 kDa
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 NCBI Official Full Name    protein-serine kinase 1
 NCBI Official Symbol    PK1 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    protein-serine kinase 1
 UniProt Protein Name    Serine/threonine-protein kinase AtPK1/AtPK6
 UniProt Synonym Protein Names   
Ribosomal-protein S6 kinase homolog 1
 UniProt Gene Name    ATPK1 [Similar Products]
 UniProt Synonym Gene Names    ATPK6; S6K1 [Similar Products]
 UniProt Entry Name    KPK1_ARATH
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 NCBI Summary for S6K1    Encodes a protein-serine kinase that phosphorylates ribosomal protein in vitro. Activation of AtS6k is regulated by 1-naphthylacetic acid and kinetin, at least in part, via a lipid kinase-dependent pathway. Involved in translational up-regulation of ribosomal proteins. Phosphorylated by PDK1. Interacts with RAPTOR1, which in turn interacts with TOR. SPK6 activity is affected by osmotic stress, and plants overexpressing S6k1 are hypersensitive to osmotic stress. The gene is expressed in all tissues examined, with highest expression level detected in metabolically active tissues.
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 UniProt Comments for S6K1    Downstream effector of TOR signaling pathway involved in osmotic stress response. Could be involved in the control of plant growth and development. Phosphorylates the ribosomal proteins P14, P16 and S6. Functions as a repressor of cell proliferation and required for maintenance of chromosome stability and ploidy levels through the RBR1-E2F pathway.
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 Research Articles on S6K1    1. S6K1 interacts with retinoblastoma related protein (RBR)1 via its N-terminal RBR binding motif, promotes its nuclear localization and consequent RBR-dependent repression of cell cycle genes through transcription factor E2FB.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-S6K1 antibodyPathways associated with anti-S6K1 antibody
 Reference Product  PubMed Publications
 TOR antibody  >6 publications with S6K1 and TOR
 PDK1 antibody  >1 publications with S6K1 and PDK1
 Products by Pathway  Pathway Diagram
 IGF1R Signaling Cascade Pathway antibodies  IGF1R Signaling Cascade Pathway Diagram
 IRS-mediated Signalling Pathway antibodies  IRS-mediated Signalling Pathway Diagram
 IRS-related Events Triggered By IGF1R Pathway antibodies  IRS-related Events Triggered By IGF1R Pathway Diagram
 Insulin Receptor Signalling Cascade Pathway antibodies  Insulin Receptor Signalling Cascade Pathway Diagram
 Insulin Resistance Pathway antibodies  Insulin Resistance Pathway Diagram
 PI3K Cascade Pathway antibodies  PI3K Cascade Pathway Diagram
 PKB-mediated Events Pathway antibodies  PKB-mediated Events Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By Insulin Receptor Pathway antibodies  Signaling By Insulin Receptor Pathway Diagram
 Signaling By Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R) Pathway antibodies  Signaling By Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R) Pathway Diagram
Organs/Tissues associated with anti-S6K1 antibody
 Organ/Tissue Name  Pubmed Publications
 Root Antibodies  >8 publications with S6K1 and Root
 Leaf Antibodies  >6 publications with S6K1 and Leaf
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