View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Add To Compare List Sheep Thrombin Polyclonal Antibody Catalog # MBS512153 Unit / Price DISCONTINUEDClick here to view our similar product(s) OR click here to contact our technical support team for suitable replacement. Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name Thrombin, Polyclonal Antibody Full Product Name Sheep anti-human Thrombin (FIIa), Biotinylated Affinity-Purified IgG Product Synonym Names Thrombin, human Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Clonality Polyclonal Host Sheep Specificity Prior to conjugation, this antibody was specific for thrombin (including prothrombin) as demonstrated by immunoelectrophoresis and ELISA. Purity/Purification Affinity purified, Biotinylated IgG.Vial containing mL of affinity-purified IgG conjugated to biotin. Total protein is 0.1 mg. Form/Format APIgG-biotin conjugate as a clear, colourless liquid. Concentration APIgG-biotin concentration is mg/mL, determined by absorbance using an extinction coefficient (E1%280) of 14. (lot specific) Immunogen Thrombin prepared from purified human prothrombin, active site blocked with PPACK. Buffer Phosphate-buffered saline containing 1 mg/mL bovine albumin and 0.1% sodium azide (w/v), pH 7.4. Preparation and Storage Store at 2 degree C to 8 degree C. Other Notes Small volumes of anti-Thrombin antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for anti-Thrombin antibody Thrombin (EC3.4.21.5, alpha-thrombin) is the product of proteolytic activation of the zymogen prothrombin. Human thrombin is a two-chain serine protease with a mass of 37 kDa. The active site is located within the heavy chain. Thrombin has a high specificity for certain arginine bonds in protein substrates. The primary substrate is fibrinogen which thrombin converts to fibrin through the cleavage of four arginyl-glycyl peptide bonds. Thrombin is also an important activator of platelets, factor XIII, Protein C and TAFI (Plasma procarboxypeptidase B). In a positive feedback mechanism, thrombin increases the rate of its own production by activation of factors VIII and V. The rate of thrombin production is subsequently limited indirectly through the activation of Protein C by thrombin, which then inactivates the activated cofactors VIII and V. The binding of thrombin to thrombomodulin on the cell surface dramatically alters thrombin's specificity, increasing it's activity toward Protein C and TAFI, and decreasing it's activity toward fibrinogen and activating cofactors VIII and V. In plasma, thrombin activity is inhibited primarily by antithrombin and to a lesser extent heparin cofactor II. The rate of inhibition by both of these inhibitors is profoundly increased in the presence of optimal concentrations of heparin. Other physiological inhibitors of thrombin in the absence of heparin include alpha2-macroglobulin and alpha1-antitrypsin1-4. Application Notes for anti-Thrombin antibody Suitable as a source of biotinylated antibodies to human thrombin (and prothrombin). TOP NCBI/Uniprot data below describe general gene information for Thrombin. It may not necessarily be applicable to this product. NCBI GI # 213487 TOP NCBI Official Full Name thrombin Protein Family Thrombin TOP Product References and Citations for anti-Thrombin antibody 1. Mann KG; Prothrombin and Thrombin; in Hemostasis and Thrombosis, 3rd Edition, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp. 184-199, J.B. Lippincott Co., Philadelphia PA, USA, 1994.2. Stubbs MT, Bode W; A Player of Many Parts: The Spotlight Falls on Thrombin's Structure; Thrombosis Research 69, pp 1-58, 1993.3. Downing MW, Bloom JW, Mann KG; Comparison of the Inhibition of Thrombin by Three Plasma Protease Inhibitors; Biochemistry 17, pp 2649- 2653, 1978.4. Weitz JI, Hudoba M, Massel D, Maranganore J, Hirsh J; Clot-bound Thrombin is Protected from Inhibition by Heparin-ATIII but is Susceptible to - ATIII independent Inhibitors; J. Clin. Invest 86, pp 385-391, 1990. 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TOP Products associated with anti-Thrombin antibody Reference Product PubMed Publications FLNA antibody >12 publications with Thrombin and FLNA Pathways associated with anti-Thrombin antibody Products by Pathway Pathway Diagram Focal Adhesion Pathway antibodies Focal Adhesion Pathway Diagram Focal Adhesion Pathway antibodies Focal Adhesion Pathway Diagram MAPK Signaling Pathway antibodies MAPK Signaling Pathway Diagram MAPK Signaling Pathway antibodies MAPK Signaling Pathway Diagram Salmonella Infection Pathway antibodies Salmonella Infection Pathway Diagram Salmonella Infection Pathway antibodies Salmonella Infection Pathway Diagram Organs/Tissues associated with anti-Thrombin antibody Organ/Tissue Name Pubmed Publications Bone Antibodies >13 publications with Thrombin and Bone Connective Tissue Antibodies >4 publications with Thrombin and Connective Tissue Heart Antibodies >3 publications with Thrombin and Heart Muscle Antibodies >3 publications with Thrombin and Muscle Brain Antibodies >3 publications with Thrombin and Brain Vascular Antibodies >2 publications with Thrombin and Vascular Embryonic Tissue Antibodies >2 publications with Thrombin and Embryonic Tissue Ear Antibodies >1 publications with Thrombin and Ear Kidney Antibodies >1 publications with Thrombin and Kidney Umbilical Cord Antibodies >1 publications with Thrombin and Umbilical Cord TOP