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anti-Lck antibody :: Rabbit anti-Human Tyrosine-protein kinase Lck Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS1498983
Unit / Price
0.05 mg  /  $160 +1 FREE 8GB USB
0.1 mg  /  $235 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Tyrosine-protein kinase Lck, Polyclonal Antibody

★Popular Item★
 Also Known As   

Rabbit anti-human Tyrosine-protein kinase Lck polyclonal Antibody

 Product Synonym Names    Leukocyte C-terminal Src kinase; Lymphocyte cell-specific protein-tyrosine kinase; Protein YT16; Proto-oncogene Lck; T cell-specific protein-tyrosine kinase; p56-LCK; LCK
 Product Gene Name   

anti-Lck antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    153390
 3D Structure    ModBase 3D Structure for P06239
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human
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 Purity/Purification    Caprylic Acid Ammonium Sulfate Precipitation Purified
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 Storage Buffer    Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
 Conjugate    Non-conjugated
 Immunogen    Recombinant human Tyrosine-protein kinase Lck protein(1-539aa)
 Santa Cruz Alternative    Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-32014 / sc-13 / sc-28882
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 Preparation and Storage    Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of anti-Lck antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-Lck antibody

   Non-receptor tyrosine-protein kinase that plays an essential role in the selection and maturation of developing T-cells in the thymus and in the function of mature T-cells. Plays a key role in T-cell antigen receptor (TCR)-linked signal transduction pathways. Constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, thereby recruiting the associated LCK protein to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosine-based activation motifs (ITAM) of the cytoplasmic tails of the TCR-gamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. Once stimulated, the TCR recruits the tyrosine kinase ZAP70, that becomes phosphorylated and activated by LCK. Following this, a large number of signaling molecules are recruited, ultimately leading to lymphokine production. LCK also contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, which leads to hyperphosphorylation and activation of LCK. Also plays a role in the IL2 receptor-linked signaling pathway that controls the T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Phosphorylates other substrates including RUNX3, PTK2B/PYK2, the microtubule-associated protein MAPT, RHOH or TYROBP.
 Applications Tested/Suitable for anti-Lck antibody   

ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF)

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 Immunohistochemistry (IHC) of anti-Lck antibody    Immunohistochemistry of paraffin-embedded human tonsil tissue using MBS1498983 at dilution of 1:100
anti-Lck antibody Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of anti-Lck antibody    Immunohistochemistry of paraffin-embedded human lymph node tissue using MBS1498983 at dilution of 1:100
anti-Lck antibody Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of anti-Lck antibody    Immunofluorescent analysis of Hela cells using MBS1498983 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
anti-Lck antibody Immunohistochemistry (IHC) (IHC) image
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 Immunoprecipitation (IP) of anti-Lck antibody    Immunoprecipitating LCK in Jurkat whole cell lysate
Lane 1: Rabbit control IgG (1ug)instead of MBS1498983 in Jurkat whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: MBS1498983 (8ug)+ Jurkat whole cell lysate (500ug)
Lane 3: Jurkat whole cell lysate (10ug)

anti-Lck antibody Immunoprecipitation (IP) (IP) image
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NCBI/Uniprot data below describe general gene information for Lck. It may not necessarily be applicable to this product.
 NCBI GI #    112789548
 NCBI GeneID    3932
 NCBI Accession #    NP_001036236.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001042771.2 [Other Products]
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 UniProt Primary Accession #    P06239 [Other Products]
 UniProt Secondary Accession #    P07100; Q12850; Q13152; Q5TDH8; Q5TDH9; Q7RTZ3; Q96DW4; Q9NYT8; D3DPP8 [Other Products]
 UniProt Related Accession #    P06239 [Other Products]
 Molecular Weight    61,190 Da
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 NCBI Official Full Name    tyrosine-protein kinase Lck
 NCBI Official Synonym Full Names    LCK proto-oncogene, Src family tyrosine kinase
 NCBI Official Symbol    LCK [Similar Products]
 NCBI Official Synonym Symbols   
LSK; YT16; IMD22; p56lck; pp58lck
[Similar Products]
 NCBI Protein Information    tyrosine-protein kinase Lck
 UniProt Protein Name    Tyrosine-protein kinase Lck
 UniProt Synonym Protein Names   
Leukocyte C-terminal Src kinase; LSK; Lymphocyte cell-specific protein-tyrosine kinase; Protein YT16; Proto-oncogene Lck; T cell-specific protein-tyrosine kinase; p56-LCK
 Protein Family    Tyrosine-protein kinase
 UniProt Gene Name    LCK [Similar Products]
 UniProt Synonym Gene Names    LSK [Similar Products]
 UniProt Entry Name    LCK_HUMAN
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 NCBI Summary for Lck    This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants, encoding the same protein, have been described. [provided by RefSeq, Jul 2008]
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 UniProt Comments for Lck    Lck: a tyrosine kinase of the Src family that is crucial to antigen-receptor signaling in lymphocytes. plays an essential role for the selection and maturation of developing T-cell in the thymus and in mature T-cell function. Is constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors and plays a key role in T-cell antigen receptor(TCR)-linked signal transduction pathways. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, and thereby recruits the associated LCK to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosines-based activation motifs (ITAMs) in the cytoplasmic tails of the TCRgamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. In addition, contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, and upon engagement of the CD2 molecule, LCK undergoes hyperphosphorylation and activation. Also plays a role in the IL2 receptor-linked signaling pathway that controls T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Binds to the cytoplasmic domain of cell surface receptors, such as CD2, CD4, CD5, CD8, CD44, CD45 and CD122. Also binds to effector molecules, such as PI4K, VAV1, RASA1, FYB and to other protein kinases including CDC2, RAF1, ZAP70 and SYK. Binds to phosphatidylinositol 3'-kinase (PI3K) from T-lymphocytes through its SH3 domain and to the tyrosine phosphorylated form of Sam68 through its SH2 domain. Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine-kinase activity. Overexpression in mice leads to thymic tumors. Aberrant expression is seen in T cell leukemias and colon cancer. The leukemic translocation t(1;7)(p34;q34) has breakpoints at the T cell receptor gene and close to the Lck promoters, can cause increased Lck expression, and in one case, point mutations. A mutated Lck has also been seen in a cell line. One patient with aberrant Lck splicing suffered from SCID-like T cell deficiency. Inhibitor: BMS-279700. Three alternatively spliced isoforms of the human proteinhave been described.

Protein type: Protein kinase, TK; Oncoprotein; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); Kinase, protein; TK group; Src family

Chromosomal Location of Human Ortholog: 1p34.3

Cellular Component: cytosol; extrinsic to internal side of plasma membrane; immunological synapse; intercellular junction; lipid raft; pericentriolar material; plasma membrane

Molecular Function: ATP binding; ATPase binding; CD4 receptor binding; CD8 receptor binding; glycoprotein binding; identical protein binding; non-membrane spanning protein tyrosine kinase activity; phosphoinositide 3-kinase binding; protein binding; protein C-terminus binding; protein kinase binding; protein phosphatase binding; protein serine/threonine phosphatase activity; protein-tyrosine kinase activity; SH2 domain binding

Biological Process: B cell receptor signaling pathway; blood coagulation; caspase activation; cellular zinc ion homeostasis; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; hemopoiesis; innate immune response; leukocyte migration; nerve growth factor receptor signaling pathway; phosphoinositide-mediated signaling; platelet activation; positive regulation of gamma-delta T cell differentiation; positive regulation of T cell activation; positive regulation of T cell receptor signaling pathway; positive regulation of tyrosine phosphorylation of Stat5 protein; protein amino acid dephosphorylation; protein amino acid phosphorylation; regulation of cell proliferation; regulation of defense response to virus by virus; regulation of lymphocyte activation; regulation of regulatory T cell differentiation; release of sequestered calcium ion into cytosol; response to drug; T cell costimulation; T cell differentiation; T cell receptor signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway; viral reproduction

Disease: Immunodeficiency 22
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 Research Articles on Lck    1. These findings demonstrate highly dynamic Lck palmitoylation kinetics that are essential for signaling downstream of the Fas receptor.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-Lck antibodyPathways associated with anti-Lck antibody
 Reference Product  PubMed Publications
 ZAP70 antibody  >401 publications with Lck and ZAP70
 LAT antibody  >141 publications with Lck and LAT
 SYK antibody  >140 publications with Lck and SYK
 VAV1 antibody  >52 publications with Lck and VAV1
 CBL antibody  >50 publications with Lck and CBL
 STAT3 antibody  >28 publications with Lck and STAT3
 PTPRC antibody  >21 publications with Lck and PTPRC
 CD8A antibody  >4 publications with Lck and CD8A
 KHDRBS1 antibody  >4 publications with Lck and KHDRBS1
 CD247 antibody  >4 publications with Lck and CD247
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Alpha-synuclein Signaling Pathway antibodies  Alpha-synuclein Signaling Pathway Diagram
 Atypical NF-kappaB Pathway antibodies  Atypical NF-kappaB Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 CD28 Co-stimulation Pathway antibodies  CD28 Co-stimulation Pathway Diagram
 CD28 Dependent PI3K/Akt Signaling Pathway antibodies  CD28 Dependent PI3K/Akt Signaling Pathway Diagram
 CD28 Dependent Vav1 Pathway antibodies  CD28 Dependent Vav1 Pathway Diagram
 CTLA4 Inhibitory Signaling Pathway antibodies  CTLA4 Inhibitory Signaling Pathway Diagram
 CXCR4-mediated Signaling Events Pathway antibodies  CXCR4-mediated Signaling Events Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
Diseases associated with anti-Lck antibodyOrgans/Tissues associated with anti-Lck antibody
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >73 publications with Lck and Inflammation
 Necrosis Antibodies  >56 publications with Lck and Necrosis
 Lung Diseases Antibodies  >39 publications with Lck and Lung Diseases
 Neoplasms, Experimental Antibodies  >37 publications with Lck and Neoplasms, Experimental
 Adenocarcinoma Antibodies  >30 publications with Lck and Adenocarcinoma
 Nervous System Diseases Antibodies  >25 publications with Lck and Nervous System Diseases
 Liver Diseases Antibodies  >20 publications with Lck and Liver Diseases
 Lung Neoplasms Antibodies  >19 publications with Lck and Lung Neoplasms
 Breast Neoplasms Antibodies  >18 publications with Lck and Breast Neoplasms
 Kidney Diseases Antibodies  >16 publications with Lck and Kidney Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1900 publications with Lck and Blood
 Thymus Antibodies  >297 publications with Lck and Thymus
 Spleen Antibodies  >124 publications with Lck and Spleen
 Lymph Node Antibodies  >63 publications with Lck and Lymph Node
 Lymph Antibodies  >59 publications with Lck and Lymph
 Lung Antibodies  >58 publications with Lck and Lung
 Brain Antibodies  >55 publications with Lck and Brain
 Liver Antibodies  >52 publications with Lck and Liver
 Heart Antibodies  >46 publications with Lck and Heart
 Vascular Antibodies  >40 publications with Lck and Vascular
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