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| Product Name
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UCH37 (UCHL5), Polyclonal Antibody
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| Also Known As
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UCH37, NT (Ubiquitin Carboxyl-terminal Hydrolase Isozyme L5, Ubiquitin Thiolesterase L5, Ubiquitin C-terminal Hydrolase UCH37, CGI-70, AD-019, UCHL5)
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| Product Synonym Names
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Anti -UCH37, NT (Ubiquitin Carboxyl-terminal Hydrolase Isozyme L5, Ubiquitin Thiolesterase L5, Ubiquitin C-terminal Hydrolase UCH37, CGI-70, AD-019, UCHL5)
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| Product Gene Name
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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 TOP
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| Chromosome Location
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Chromosome: 1; NC_000001.10 (192981496..193029237, complement). Location: 1q32
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| OMIM
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610667
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| 3D Structure
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ModBase 3D Structure for Q9Y5K5
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| Clonality
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Polyclonal
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| Isotype
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IgG
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| Host
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Rabbit
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| Species Reactivity
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Human, Mouse
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| Specificity
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Recognizes human UCHL5. Species Crossreactivity: mouse.
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| Purity/Purification
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Affinity Purified
Purified by Protein G affinity chromatography.
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| Form/Format
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Supplied as a liquid in PBS, 0.09% sodium azide.
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| Immunogen
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Synthetic peptide selected from the N-terminal region of human UCHL5 (KLH).
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| Preparation and Storage
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May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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| Other Notes
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Small volumes of anti-UCHL5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Covalent attachment of the C-terminus of ubiquitin to cellular proteins plays a role in a variety of cellular processes. Ubiquitin C-terminal hydrolysis is catalyzed by deubiquitinating (DUB) enzymes and is necessary for several functions, including liberation of monomeric ubiquitin from the precursors encoded by ubiquitin genes and recycling of ubiquitin monomers. There are 2 distinct families of DUBs, ubiquitin-specific proteases (UBPs) and ubiquitin C-terminal hydrolases (UCHs). Mayer and Wilkinson (1989) identified 4 distinct UCH activities from bovine thymus. All 4 were thiol proteases and had high-affinity binding sites for ubiquitin. Wilkinson et al. (1989) purified the predominant isozyme, UCHL3, and raised antibodies against it. By screening a human B-cell expression library with the antibodies, the authors isolated cDNAs encoding human UCHL3. Sequence comparisons revealed that the sequence of the predicted 230aa human UCHL3 protein is 54% identical to that of UCHL1.
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| Product Categories/Family for anti-UCHL5 antibody
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Antibodies; Abs to Ubiquitin
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| Applications Tested/Suitable for anti-UCHL5 antibody
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ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
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| Application Notes for anti-UCHL5 antibody
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Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100
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TOP
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| NCBI/Uniprot data below describe general gene information for UCHL5. It may not necessarily be applicable to this product. |
| NCBI GI #
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312922359
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| NCBI GeneID
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51377
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| NCBI Accession #
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NP_001186190.1
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[Other Products]
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| NCBI GenBank Nucleotide #
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NM_001199261.1
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[Other Products]
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| UniProt Primary Accession #
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Q9Y5K5
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[Other Products]
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| UniProt Secondary Accession #
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Q5LJA6; Q5LJA7; Q8TBS4; Q96BJ9; Q9H1W5; Q9P0I3; Q9UQN2
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[Other Products]
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| UniProt Related Accession #
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Q9Y5K5
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[Other Products]
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| Molecular Weight
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37,607 Da
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[Similar Products]
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TOP
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| NCBI Official Full Name
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ubiquitin carboxyl-terminal hydrolase isozyme L5 isoform 2
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| NCBI Official Synonym Full Names
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ubiquitin carboxyl-terminal hydrolase L5
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| NCBI Official Symbol
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UCHL5
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[Similar Products]
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| NCBI Official Synonym Symbols
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UCH37; CGI-70; INO80R; UCH-L5
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[Similar Products]
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| NCBI Protein Information
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ubiquitin carboxyl-terminal hydrolase isozyme L5; INO80 complex subunit R; ubiquitin thioesterase L5; ubiquitin C-terminal hydrolase UCH37; ubiquitin carboxyl-terminal esterase L5
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| UniProt Protein Name
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Ubiquitin carboxyl-terminal hydrolase isozyme L5
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| UniProt Synonym Protein Names
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Ubiquitin C-terminal hydrolase UCH37; Ubiquitin thioesterase L5
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| Protein Family
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Ubiquitin carboxyl-terminal hydrolase
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| UniProt Gene Name
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UCHL5
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[Similar Products]
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| UniProt Synonym Gene Names
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UCH37; UCH-L5
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[Similar Products]
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| UniProt Entry Name
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UCHL5_HUMAN
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| UniProt Comments for UCHL5
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UCHL5: Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Putative regulatory component of the INO80 complex; however is inactive in the INO80 complex and is activated by a transient interaction of the INO80 complex with the proteasome via ADRM1. Belongs to the peptidase C12 family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Ubiquitin-specific protease; EC 3.4.19.12; Ubiquitin conjugating system; Protease
Chromosomal Location of Human Ortholog: 1q32
Cellular Component: proteasome complex; cytosol; nucleus
Molecular Function: omega peptidase activity; protein binding; endopeptidase inhibitor activity; ubiquitin-specific protease activity
Biological Process: ubiquitin-dependent protein catabolic process; protein deubiquitination; regulation of transcription, DNA-dependent; transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; midbrain development; negative regulation of transforming growth factor beta receptor signaling pathway; forebrain morphogenesis; DNA repair; lateral ventricle development; DNA recombination
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TOP
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| Research Articles on UCHL5
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1. UCH37 over-expression is associated with hepatocellular carcinoma recurrence.
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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| Products associated with anti-UCHL5 antibody | Pathways associated with anti-UCHL5 antibody |
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| Diseases associated with anti-UCHL5 antibody | Organs/Tissues associated with anti-UCHL5 antibody |
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Antibody 
