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anti-UBC9 antibody :: Rabbit Ubc9 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS616215 anti-UBC9 antibody
Unit / Price
0.1 mg  /  $620 +1 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Ubc9, Polyclonal Antibody

 Also Known As   

Ubc9 (Ubiquitin-Conjugating Enzyme 9)

 Product Synonym Names    Anti -Ubc9 (Ubiquitin-Conjugating Enzyme 9)
 Product Gene Name   

anti-UBC9 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 4; NC_003075.7 (13915820..13917420, complement)
 3D Structure    ModBase 3D Structure for P35132
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human, Mouse, Rat
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 Specificity    Reacts with human, mouse, rat and golden hamster ubiquitin-conjugating enzyme 9 (Ubc9) protein. On Western blots, it identifies the target band at ~18kD. A band at ~13kD is observed in mouse spleen homogenates. Reactivity has been confirmed HEK293, HepG2, HL-60 cell lysates and mouse spleen homogenates.
 Purity/Purification    Affinity Purified
Epitope-affinity purified from rabbit antiserum.
 Form/Format    Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
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 Immunogen    Synthetic peptide derived from an internal region of the human, mouse, rat and golden hamster Ubc9 proteins.
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 Preparation and Storage    May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
 Other Notes    Small volumes of anti-UBC9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-UBC9 antibody

   Ubiquitin-conjugating enzyme 9 (Ubc9)1-3 is a component of the ubiquitin-mediated proteolytic SUMO (sentrin) pathway that targets proteins for degradation by the 26S proteasome, mediates endocytosis and directs protein subcellular localization.4,5 Ubc9 primarily mediates SUMOlation but not ubiquitinylation.6,7 An E3 ubiquitin ligase systematically transfers Ub and Ublike molecules from E2 conjugating enzymes to the targeted substrate.8 Ubc9, like other E2 family members, contains a conserved cysteine residue that is required for the thio ester formation between Ub-like proteins, and the E2 member, and it shares a conserved Ubc domain of approximately 16kD.9 More than 30 different vertebrate proteins are potential candidates for SUMOlation by Ubc9.10 Many Ubc9-interacting proteins contain a PEST sequence, a stretch of at least 12 amino acids rich in P, E, D, S, and T amino acid residues.10-13 Substrates for Ubc9 include transcription factors E12 and E47 and mitotic regulators RanBP2 and RanGAP1, indicating that Ubc9 may regulate various cellular processes including cell cycle progression and differentiation.14,15 Many Ubc9-interacting proteins have been implicated in the maintenance of vertebrate genome integrity, including the tumor suppressor protein p53,16 the promyelocytic leukemia protein (Pml),17 the poly(ADP-ribose) polymerase PARP18 and the homologous recombination proteins Rad51/52.13,16,19 Ubc9 is predominantly present in the nucleus, and at the nuclear pore complex.10, 20-22 Ubc9's distribution across the nuclear membrane, nuclear versus cytoplasmic, is important in controlling the subcellular localization of SUMOs and/or SUMOlated proteins, the assembly of Pml bodies, and the homologous recombination process mediated by Rad51.13
 Product Categories/Family for anti-UBC9 antibody    Antibodies; Abs to Ubiquitin
 Applications Tested/Suitable for anti-UBC9 antibody   

Western Blot (WB)

 Application Notes for anti-UBC9 antibody    Suitable for use in Western Blot.
Dilution: Western Blotting: 1-3ug/ml
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NCBI/Uniprot data below describe general gene information for UBC9. It may not necessarily be applicable to this product.
 NCBI GI #    30687803
 NCBI GeneID    828909
 NCBI Accession #    NP_849462.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_179131.2 [Other Products]
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 UniProt Primary Accession #    P35132 [Other Products]
 UniProt Secondary Accession #    Q42014; Q4TZ01; Q8RXQ0; Q9FPH9 [Other Products]
 UniProt Related Accession #    S32674 [Other Products]
 Molecular Weight    16,553 Da [Similar Products]
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 NCBI Official Full Name    SUMO-conjugating enzyme UBC9
 NCBI Official Symbol    UBC9 [Similar Products]
 NCBI Official Synonym Symbols   
T13J8.70; T13J8_70; UBC9; ubiquitin conjugating enzyme 9; UBIQUITIN-PROTEIN LIGASE
[Similar Products]
 NCBI Protein Information    SUMO-conjugating enzyme UBC9
 UniProt Protein Name    SUMO-conjugating enzyme UBC9
 UniProt Synonym Protein Names   
UBCAT4B; Ubiquitin carrier protein 9; Ubiquitin-conjugating enzyme E2 9; Ubiquitin-conjugating enzyme E2-17 kDa; Ubiquitin-protein ligase 9
 UniProt Gene Name    UBC9 [Similar Products]
 UniProt Synonym Gene Names    UBC4B [Similar Products]
 UniProt Entry Name    UBC9_ARATH
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 NCBI Summary for UBC9    ubiquitin conjugating enzyme
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 UniProt Comments for UBC9    Function: Accepts the ubiquitin-like protein SUMO/SMT3 from the E1 complex and catalyzes its covalent attachment to other proteins. Mediates the selective degradation of short-lived and abnormal proteins.

Catalytic activity: ATP + SUMO + protein lysine = AMP + diphosphate + protein N-SUMOyllysine.

Pathway: Protein modification; protein sumoylation.

Subunit structure: Interacts with CHIP. Ref.8

Tissue specificity: Highest expression in young stems and old leaves. Lowest levels in floral buds, anthers and young leaves. Ref.3

Developmental stage: Up-regulated during senescence, but not during the G0 to S phase transition. Ref.2

Induction: Not induced by heat shock or wounding. Ref.2

Sequence similarities: Belongs to the ubiquitin-conjugating enzyme family.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-UBC9 antibodyOrgans/Tissues associated with anti-UBC9 antibody
 Products by Pathway  Pathway Diagram
 APC/C-mediated Degradation Of Cell Cycle Proteins Pathway antibodies  APC/C-mediated Degradation Of Cell Cycle Proteins Pathway Diagram
 APC/C:Cdc20 Mediated Degradation Of Securin Pathway antibodies  APC/C:Cdc20 Mediated Degradation Of Securin Pathway Diagram
 APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies  APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
 APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway antibodies  APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway Diagram
 Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies  Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
 Autodegradation Of Cdh1 By Cdh1:APC/C Pathway antibodies  Autodegradation Of Cdh1 By Cdh1:APC/C Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Conversion From APC/C:Cdc20 To APC/C:Cdh1 In Late Anaphase Pathway antibodies  Conversion From APC/C:Cdc20 To APC/C:Cdh1 In Late Anaphase Pathway Diagram
 Regulation Of APC/C Activators Between G1/S And Early Anaphase Pathway antibodies  Regulation Of APC/C Activators Between G1/S And Early Anaphase Pathway Diagram
 Regulation Of Mitotic Cell Cycle Pathway antibodies  Regulation Of Mitotic Cell Cycle Pathway Diagram
 Organ/Tissue Name  Pubmed Publications
 Cell Culture Antibodies  >9 publications with UBC9 and Cell Culture
 Bud Antibodies  >3 publications with UBC9 and Bud
 Whole Plant Antibodies  >1 publications with UBC9 and Whole Plant
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