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anti-VHL antibody :: Rabbit VHL Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS9206245
Unit / Price
0.08 mL  /  $155 +1 FREE 8GB USB
0.4 mL  /  $340 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

VHL, Polyclonal Antibody

 Also Known As   

VHL Antibody (N-term)

 Product Synonym Names    Von Hippel-Lindau disease tumor suppressor; Protein G7; pVHL; VHL
 Product Gene Name   

anti-VHL antibody

[Similar Products]
 Antibody/Peptide Pairs    VHL peptide (MBS9226147) is used for blocking the activity of VHL antibody (MBS9206245)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence Positions    43-71
 OMIM    144700
 3D Structure    ModBase 3D Structure for P40337
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 Clonality    Polyclonal
 Isotype    Rabbit Ig
 Host    Rabbit
 Species Reactivity    Human, mouse (Predicted Reactivity: Rat)
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 Specificity    This VHL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 43-71 amino acids from the N-terminal region of human VHL.
 Purity/Purification    Purified Rabbit Polyclonal Antibody (Pab)
 Form/Format    Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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 Function    Involved in the ubiquitination and subsequent proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Seems to act as a target recruitment subunit in the E3 ubiquitin ligase complex and recruits hydroxylated hypoxia- inducible factor (HIF) under normoxic conditions. Involved in transcriptional repression through interaction with HIF1A, HIF1AN and histone deacetylases. Ubiquitinates, in an oxygen-responsive manner, ADRB2.
 Cellular Location    Isoform 1: Cytoplasm. Membrane; Peripheral membrane protein. Nucleus. Note=Found predominantly in the cytoplasm and with less amounts nuclear or membrane-associated Colocalizes with ADRB2 at the cell membrane
 Tissue Location    Expressed in the adult and fetal brain and kidney
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 Preparation and Storage    Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
 Other Notes    Small volumes of anti-VHL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-VHL antibody

   Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of VHL gene is the basis of familial inheritance of VHL syndrome. The protein is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein.
 Product Categories/Family for anti-VHL antibody    Cancer; Cell Biology; Metabolism
 Applications Tested/Suitable for anti-VHL antibody   

Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC-P), Immunofluorescence (IF), Flow Cytometry (FC/FACS)

 Application Notes for anti-VHL antibody    WB~~1:1000
IHC-P~~1:10~50
IF~~1:10~50
FC~~1:10~50
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 Western Blot of anti-VHL antibody    Anti-VHL Antibody (N-term) at 1:2000 dilution + F9 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG,(H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 24 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
anti-VHL antibody Western Blot (WB) image
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 Western Blot (WB) of anti-VHL antibody    Western blot analysis of VHL antibody (N-term) in HepG2 cell line lysates (35ug/lane). VHL (arrow) was detected using the purified Pab.
anti-VHL antibody Western Blot (WB) (WB) image
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 Immunohistochemistry (IHC) of anti-VHL antibody    VHL Antibody (N-term) (RB18667) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the VHL Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
anti-VHL antibody Immunohistochemistry (IHC) (IHC) image
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 Immunofluorescence (IF) of anti-VHL antibody    Confocal immunofluorescent analysis of VHL Antibody (N-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
anti-VHL antibody Immunofluorescence (IF) image
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 Flow Cytometry (FC/FACS) of anti-VHL antibody    VHL Antibody (N-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
anti-VHL antibody Flow Cytometry (FC/FACS) (FC/FACS) image
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NCBI/Uniprot data below describe general gene information for VHL. It may not necessarily be applicable to this product.
 NCBI GI #    4507891
 NCBI GeneID    7428
 NCBI Accession #    NP_000542.1 [Other Products]
 NCBI Related Accession #    NP_937799.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000551.3 [Other Products]
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 UniProt Primary Accession #    P40337 [Other Products]
 UniProt Secondary Accession #    Q13599; Q6PDA9; B2RE45 [Other Products]
 UniProt Related Accession #    P40337 [Other Products]
 Molecular Weight    24153
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 NCBI Official Full Name    von Hippel-Lindau disease tumor suppressor isoform 1
 NCBI Official Synonym Full Names    von Hippel-Lindau tumor suppressor, E3 ubiquitin protein ligase
 NCBI Official Symbol    VHL [Similar Products]
 NCBI Official Synonym Symbols   
RCA1; VHL1; pVHL; HRCA1
[Similar Products]
 NCBI Protein Information    von Hippel-Lindau disease tumor suppressor
 UniProt Protein Name    Von Hippel-Lindau disease tumor suppressor
 UniProt Synonym Protein Names   
Protein G7; pVHL
 Protein Family    Von Hippel-Lindau disease tumor suppressor
 UniProt Gene Name    VHL [Similar Products]
 UniProt Entry Name    VHL_HUMAN
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 NCBI Summary for VHL    Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of this gene is the basis of familial inheritance of VHL syndrome. The protein encoded by this gene is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
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 UniProt Comments for VHL    VHL: Involved in the ubiquitination and subsequent proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Seems to act as target recruitment subunit in the E3 ubiquitin ligase complex and recruits hydroxylated hypoxia- inducible factor (HIF) under normoxic conditions. Involved in transcriptional repression through interaction with HIF1A, HIF1AN and histone deacetylases. Ubiquitinates, in an oxygen-responsive manner, ADRB2. Component of the VCB (VHL-Elongin BC-CUL2) complex; this complex acts as a ubiquitin-ligase E3 and directs proteasome- dependent degradation of targeted proteins. Interacts with CUL2; this interaction is dependent on the integrity of the trimeric VBC complex. Interacts (via the beta domain) with HIF1A (via the NTAD domain); this interaction mediates degradation of HIF1A in normoxia and, in hypoxia, prevents ubiqitination and degradation of HIF1A by mediating hypoxia-induced translocation to the nucleus, a process which requires a hypoxia-dependent regulatory signal. Interacts with ADRB2; the interaction, in normoxia, is dependent on hydroxylation of ADRB2 and the subsequent VCB- mediated ubiquitination and degradation of ADRB2. Under hypoxia, hydroxylation, interaction with VHL, ubiquitination and subsequent degradation of ADRB2 are dramatically decreased. Interacts with RNF139, USP33 and PHF17. Found in a complex composed of LIMD1, VHL, EGLN1/PHD2, TCEB2 AND CUL2. Isoform 1 and isoform 3 interact with LIMD1 (via LIM zinc-binding 2), AJUBA (via LIM domains) and WTIP (via LIM domains). Interacts with EPAS1. Expressed in the adult and fetal brain and kidney. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Tumor suppressor; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 3p25.3

Cellular Component: nucleoplasm; intermediate filament cytoskeleton; mitochondrion; membrane; endoplasmic reticulum; cytosol; nucleus

Molecular Function: protein binding; enzyme binding; ubiquitin-protein ligase activity; transcription factor binding

Biological Process: negative regulation of cell proliferation; regulation of transcription, DNA-dependent; protein stabilization; positive regulation of transcription, DNA-dependent; cell morphogenesis; protein ubiquitination; negative regulation of transcription from RNA polymerase II promoter; proteolysis; positive regulation of cell differentiation; negative regulation of apoptosis

Disease: Erythrocytosis, Familial, 2; Von Hippel-lindau Syndrome; Renal Cell Carcinoma, Nonpapillary; Pheochromocytoma
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Product References and Citations for anti-VHL antibody

   Olmos,G., Cell. Mol. Life Sci. 66 (13), 2167-2180 (2009) Hatzimichael,E., Clin Lymphoma Myeloma 9 (3), 239-242 (2009) Luu,V.D., Clin. Cancer Res. 15 (10), 3297-3304 (2009)
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 Research Articles on VHL    1. VHL can inhibit SARS-CoV replication by regulating nsp16 ubiquitination and promoting its degradation.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-VHL antibodyDiseases associated with anti-VHL antibody
 Reference Product  PubMed Publications
 HIF1A antibody  >545 publications with VHL and HIF1A
 CUL2 antibody  >58 publications with VHL and CUL2
 EGLN1 antibody  >46 publications with VHL and EGLN1
 RBX1 antibody  >30 publications with VHL and RBX1
 EPAS1 antibody  >19 publications with VHL and EPAS1
 TCEB1 antibody  >7 publications with VHL and TCEB1
 HIF3A antibody  >6 publications with VHL and HIF3A
 TCEB2 antibody  >3 publications with VHL and TCEB2
 UBC antibody  >2 publications with VHL and UBC
 Disease Name  Pubmed Publications
 von Hippel-Lindau Disease Antibodies  >1647 publications with VHL and von Hippel-Lindau Disease
 Carcinoma Antibodies  >1452 publications with VHL and Carcinoma
 Carcinoma, Renal Cell Antibodies  >1250 publications with VHL and Carcinoma, Renal Cell
 Kidney Diseases Antibodies  >1102 publications with VHL and Kidney Diseases
 Kidney Neoplasms Antibodies  >1098 publications with VHL and Kidney Neoplasms
 Cardiovascular Diseases Antibodies  >1067 publications with VHL and Cardiovascular Diseases
 Pheochromocytoma Antibodies  >512 publications with VHL and Pheochromocytoma
 Brain Diseases Antibodies  >199 publications with VHL and Brain Diseases
 Chromosome Aberrations Antibodies  >157 publications with VHL and Chromosome Aberrations
 Death Antibodies  >127 publications with VHL and Death
Organs/Tissues associated with anti-VHL antibody
 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >1175 publications with VHL and Kidney
 Vascular Antibodies  >556 publications with VHL and Vascular
 Blood Antibodies  >383 publications with VHL and Blood
 Brain Antibodies  >172 publications with VHL and Brain
 Pancreas Antibodies  >141 publications with VHL and Pancreas
 Liver Antibodies  >112 publications with VHL and Liver
 Eye Antibodies  >106 publications with VHL and Eye
 Lung Antibodies  >104 publications with VHL and Lung
 Bone Antibodies  >102 publications with VHL and Bone
 Ear Antibodies  >62 publications with VHL and Ear
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