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anti-VIM antibody :: Sheep Vimentin Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS512168 anti-VIM antibody
Unit / Price
10 mg  /  $285 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Vimentin, Polyclonal Antibody

★Popular Item★
 Also Known As   

Sheep anti-human Vimentin, Whole IgG from antiserum

 Product Synonym Names    Vimentin, human
 Product Gene Name   

anti-VIM antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 10; NC_000010.10 (17270258..17279592). Location: 10p13
 OMIM    193060
 3D Structure    ModBase 3D Structure for P08670
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 Clonality    Polyclonal
 Host    Sheep
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 Specificity    This antibody is specific for vimentin as demonstrated by immunoelectrophoresis and ELISA.
 Purity/Purification    Purified IgG.
Vial containing ml of whole IgG representing approximately 1 ml of antiserum. Total protein is 10 mg.
 Form/Format    Whole IgG, clear liquid.
 Concentration    IgG concentration is mg/ml, determined by absorbance using an extinction coefficient (E1%280) of 13.4. (lot specific)
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 Immunogen    Purified recombinant human vimentin produced in bacteria. This protein constitutes a soluble 20 kDa fragment from the amino-terminus of vimentin.
 Buffer    10 mM HEPES, pH 7.4, 150 mM NaCl, 50% (v/v) glycerol.
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 Preparation and Storage    Store between -10 and -20 degree C. Product will become viscous but will not freeze. Avoid storage in frost-free freezers. Keep vial tightly capped. Allow product to warm to room temperature and gently mix before use.
 Other Notes    Small volumes of anti-VIM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-VIM antibody

   Intermediate filaments (IFs), along with microfilaments and microtubules, are the three major filament systems that form the architectural basis of most eukaryotic cells. Vimentin, with a molecular weight of 58 kDa, is a Type III intermediate filament found in cells of mesenchymal origin including endothelial cells, megakaryocytes and platelets as well as in most cultured cell lines. As with other IFs, vimentin provides a physical linkage between the plasma membrane and the nuclear envelope. The carboxy-terminus of vimentin associates with the lamin B of the nuclear envelope whereas the amino-terminus associates with the plasma membrane either directly or indirectly. In general, it is believed that IFs, including vimentin, provide a network onto which various enzyme systems, intracellular structures and organelles are spatially arranged within the cytoplasm. IFs may also play an active role in the re-organization of intracellular components in response to extracellular signals via their disassembly and reassembly. In normal vascular endothelium, vimentin, along with other cytoskeletal proteins, are typically not exposed to extracellular plasma proteins. However, with damage to endothelial cells, exposure of these cytoskeletal components may play a role in thrombogenesis or inflammatory responses. For example, it has been demonstrated that vimentin can bind complement components as wells as immunoglobulins. Vimetin has also been shown to bind vitronectin and vitronectin/PAI-1 complexes in LPS-damaged endothelial cells.1-3
 Application Notes for anti-VIM antibody    Suitable for use as a source of antibodies to vimentin.
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NCBI/Uniprot data below describe general gene information for VIM. It may not necessarily be applicable to this product.
 NCBI GI #    62414289
 NCBI GeneID    7431
 NCBI Accession #    NP_003371.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_003380.2 [Other Products]
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 UniProt Primary Accession #    P08670 [Other Products]
 UniProt Secondary Accession #    Q15867; Q15868; Q15869; Q548L2; Q6LER9; Q8N850; Q96ML2; Q9NTM3; B0YJC2; D3DRU4 [Other Products]
 UniProt Related Accession #    P08670; Q53HU8 [Other Products]
 Molecular Weight    53521 Da [Similar Products]
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 NCBI Official Full Name    vimentin
 NCBI Official Synonym Full Names    vimentin
 NCBI Official Symbol    VIM [Similar Products]
 NCBI Official Synonym Symbols   
FLJ36605
[Similar Products]
 NCBI Protein Information    vimentin; OTTHUMP00000019224; OTTHUMP00000019225
 UniProt Protein Name    Vimentin
 Protein Family    Vimentin
 UniProt Gene Name    VIM [Similar Products]
 UniProt Entry Name    VIME_HUMAN
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 NCBI Summary for VIM    This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.
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 UniProt Comments for VIM    Function: Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells.

Subunit structure: Homopolymer. Interacts with HCV core protein. Interacts with LGSN and SYNM. Interacts (via rod region) with PLEC (via CH 1 domain)

By similarity. Interacts with SLC6A4. Ref.22 Ref.35

Tissue specificity: Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines. Ref.12 Ref.17

Post-translational modification: Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin

By similarity. One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments.

Sequence similarities: Belongs to the intermediate filament family.

Sequence caution: The sequence BAB71275.1 differs from that shown. Reason: Intron retention.
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Product References and Citations for anti-VIM antibody

   1. Steinert, P.M. and Roop, D.R. Molecular and cellular biology of intermediate filaments. Ann. Rev. Biochem. 57:593-625, 1988.
2. Albrect, D.L., Mills, J.W. and Noelle, R.J. Membrane Ig-Cytoskeletal interatctions: receptor cross-linking results in the formation of extensive filamentous arrays of vimentin. J. Immunol. 144:3251-3256, 1990.
3. Podor, T.J. and Loskutoff, D.J. Binding of PAI-1/vitronectin complexes to the intermediate filament cytoskeleton of endotoxin injured endothelial cells. Fibrinolysis (suppl.), 4:263a, 1990.
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 Research Articles on VIM    1. eEF1gamma, in addition to its role in translation elongation complex, is involved in regulating Vimentin gene by contacting both pol II and the Vimentin promoter region and shuttling/nursing the Vimentin mRNA
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-VIM antibodyPathways associated with anti-VIM antibody
 Reference Product  PubMed Publications
 GFAP antibody  >113 publications with VIM and GFAP
 CASP3 antibody  >6 publications with VIM and CASP3
 DSP antibody  >4 publications with VIM and DSP
 NEFL antibody  >2 publications with VIM and NEFL
 KRT20 antibody  >2 publications with VIM and KRT20
 CDH5 antibody  >2 publications with VIM and CDH5
 YWHAZ antibody  >2 publications with VIM and YWHAZ
 PLA2G4A antibody  >1 publications with VIM and PLA2G4A
 PDLIM1 antibody  >1 publications with VIM and PDLIM1
 FABP4 antibody  >1 publications with VIM and FABP4
 Products by Pathway  Pathway Diagram
 Alpha6-Beta4 Integrin Signaling Pathway antibodies  Alpha6-Beta4 Integrin Signaling Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptotic Cleavage Of Cellular Proteins Pathway antibodies  Apoptotic Cleavage Of Cellular Proteins Pathway Diagram
 Apoptotic Execution Phase Pathway antibodies  Apoptotic Execution Phase Pathway Diagram
 Aurora B Signaling Pathway antibodies  Aurora B Signaling Pathway Diagram
 Caspase Cascade In Apoptosis Pathway antibodies  Caspase Cascade In Apoptosis Pathway Diagram
 Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway antibodies  Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway Diagram
 Muscle Contraction Pathway antibodies  Muscle Contraction Pathway Diagram
 Striated Muscle Contraction Pathway antibodies  Striated Muscle Contraction Pathway Diagram
 Striated Muscle Contraction Pathway antibodies  Striated Muscle Contraction Pathway Diagram
Diseases associated with anti-VIM antibodyOrgans/Tissues associated with anti-VIM antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >246 publications with VIM and Neoplasms
 Movement Disorders Antibodies  >215 publications with VIM and Movement Disorders
 Neurodegenerative Diseases Antibodies  >156 publications with VIM and Neurodegenerative Diseases
 Carcinoma Antibodies  >88 publications with VIM and Carcinoma
 Immune System Diseases Antibodies  >85 publications with VIM and Immune System Diseases
 Leukemia Antibodies  >48 publications with VIM and Leukemia
 Leukemia, Myeloid Antibodies  >43 publications with VIM and Leukemia, Myeloid
 Adenocarcinoma Antibodies  >43 publications with VIM and Adenocarcinoma
 Inflammation Antibodies  >37 publications with VIM and Inflammation
 Lymphoma Antibodies  >37 publications with VIM and Lymphoma
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >408 publications with VIM and Brain
 Blood Antibodies  >169 publications with VIM and Blood
 Muscle Antibodies  >93 publications with VIM and Muscle
 Nerve Antibodies  >65 publications with VIM and Nerve
 Bone Antibodies  >64 publications with VIM and Bone
 Kidney Antibodies  >48 publications with VIM and Kidney
 Bone Marrow Antibodies  >46 publications with VIM and Bone Marrow
 Liver Antibodies  >42 publications with VIM and Liver
 Vascular Antibodies  >35 publications with VIM and Vascular
 Eye Antibodies  >32 publications with VIM and Eye
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