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anti-WASF1 antibody :: Rabbit WAVE1 (N-terminal region) Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS472069
Testing Data
Unit / Price
0.1 mL  /  $280 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

WAVE1 (N-terminal region) (WASF1), Polyclonal Antibody

 Also Known As   

WAVE1 (N-terminal region)

 Product Synonym Names    Wiskott-Aldrich syndrome verproline-homologous protein 1; Scar1; WASF1
 Product Gene Name   

anti-WASF1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 6; NC_000006.11 (110421022..110501207, complement). Location: 6q21
 OMIM    605035
 3D Structure    ModBase 3D Structure for Q92558
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 Clonality    Polyclonal
 Host    Rabbit
 Species Reactivity    Human, Rat, Mouse
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 Specificity    This antibody was affinity purified using WAVE1 (Nterminal region) peptide (without carrier). The antibody detects an 80 kDa* protein corresponding to the molecular mass of WAVE1 on SDS-PAGE immunoblots of human SYF cSrc-transformed, HUVEC, and K562 cells, as well as rat PC12 cells and mouse C2C12 cells.
 Purity/Purification    Affinity Purified
 Form/Format    Rabbit polyclonal, affinity-purified antibody is supplied in 100 ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
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 Immunogen    A synthetic peptide (coupled to KLH) corresponding to amino acid residues in the N-terminal half of human WAVE1. This sequence has high homology with similar regions in rat and mouse WAVE1, and has less than 50% homology to similar regions in human WAVE2 and WAVE3.
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 Preparation and Storage    Store at -20 degree C. Do not aliquot. Stable for 1 year.
 Other Notes    Small volumes of anti-WASF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-WASF1 antibody

   The Wiskott-Aldrich syndrome protein (WASP) family is involved in various pathways that regulate actin cytoskeletal organization. This family includes WASP, N-WASP, and three WAVE/SCAR isoforms, WAVE1, 2, and 3. WAVE proteins play key roles in actin-mediated cell events, such as membrane ruffling and lamellipodia formation. WAVEs contain an Nterminal WAVE homology domain, a basic domain, a Proline-rich region, and carboxy terminal verprolin, cofilin, and acidic (VCA) region. WAVEs are thought to act downstream of the Rac GTPase, connecting Rac activation to induction of Arp 2/3-mediated actin polymerization. Regulation of WAVE activity can occur through tyrosine phosphorylation. Src phosphorylation of WAVE1 at Tyr-125 enhances binding to the Arp2/3 complex, and is required for WAVE inhibition of Arp2/3-mediated stress fiber formation. By contrast, WAVE2 phosphorylation of Tyr-150 by Abl may enhance Arp2/3 complex actin nucleation and microspike formation in fibroblasts. Thus, site-specific tyrosine phosphorylation may be important for controlling specific activities of WAVE proteins.
 Applications Tested/Suitable for anti-WASF1 antibody   

ELISA, Western Blot

 Application Notes for anti-WASF1 antibody    ELISA: 1:2000
Western Blot: 1:1000
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 Testing Data of anti-WASF1 antibody    anti-WASF1 antibody Testing Data image
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NCBI/Uniprot data below describe general gene information for WASF1. It may not necessarily be applicable to this product.
 NCBI GI #    68161504
 NCBI GeneID    8936
 NCBI Accession #    NP_001020107.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001024936.1 [Other Products]
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 UniProt Primary Accession #    Q92558 [Other Products]
 UniProt Secondary Accession #    Q5SZK7; E1P5F2 [Other Products]
 UniProt Related Accession #    Q5SZK3; Q5SZK4; Q5SZK5; Q92558 [Other Products]
 Molecular Weight    61,652 Da [Similar Products]
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 NCBI Official Full Name    wiskott-Aldrich syndrome protein family member 1
 NCBI Official Synonym Full Names    WAS protein family, member 1
 NCBI Official Symbol    WASF1 [Similar Products]
 NCBI Official Synonym Symbols   
WAVE; SCAR1; WAVE1; FLJ31482; KIAA0269
[Similar Products]
 NCBI Protein Information    wiskott-Aldrich syndrome protein family member 1; protein WAVE-1; OTTHUMP00000016990; OTTHUMP00000016992; WASP family protein member 1; homology of dictyostelium scar 1; verprolin homology domain-containing protein 1
 UniProt Protein Name    Wiskott-Aldrich syndrome protein family member 1
 UniProt Synonym Protein Names   
Protein WAVE-1; Verprolin homology domain-containing protein 1
 Protein Family    Wiskott-Aldrich syndrome protein family
 UniProt Gene Name    WASF1 [Similar Products]
 UniProt Synonym Gene Names    KIAA0269; SCAR1; WAVE1 [Similar Products]
 UniProt Entry Name    WASF1_HUMAN
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 NCBI Summary for WASF1    The protein encoded by this gene, a member of the Wiskott-Aldrich syndrome protein (WASP)-family, plays a critical role downstream of Rac, a Rho-family small GTPase, in regulating the actin cytoskeleton required for membrane ruffling. It has been shown to associate with an actin nucleation core Arp2/3 complex while enhancing actin polymerization in vitro. Wiskott-Aldrich syndrome is a disease of the immune system, likely due to defects in regulation of actin cytoskeleton. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
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 UniProt Comments for WASF1    Function: Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex.

Subunit structure: Component of the WAVE1 complex composed of ABI2, CYFIP1 or CYFIP2, BRK1, NCKAP1 and WASF1/WAVE1. Within the complex, a heterdimer containing NCKAP1 and CYFIP1 interacts with a heterotrimer formed by WAVE1, ABI2 and BRK1. CYFIP2 binds to activated RAC1 which causes the complex to dissociate, releasing activated WASF1. The complex can also be activated by NCK1

By similarity. Binds actin and the Arp2/3 complex. Interacts with BAIAP2. Ref.7 Ref.8

Subcellular location: Cytoplasm › cytoskeleton. Note: Dot-like pattern in the cytoplasm. Concentrated in Rac-regulated membrane-ruffling areas.

Tissue specificity: Highly expressed in brain. Lowly expressed in testis, ovary, colon, kidney, pancreas, thymus, small intestine and peripheral blood.

Domain: Binds the Arp2/3 complex through the C-terminal region and actin through verprolin homology (VPH) domain.

Sequence similarities: Belongs to the SCAR/WAVE family.Contains 1 WH2 domain.

Sequence caution: The sequence BAA13399.2 differs from that shown. Reason: Erroneous initiation.
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Product References and Citations for anti-WASF1 antibody

   • Ardern, H. et al. (2006) Cell Motil. Cytosk. 63:6.
• Leng, Y. et al. (2005) PNAS 102(4):1098.
• Suetsugu, S. et al. (1999) Bioch. Biophs. Res. Comm. 260:296.
• Miki, H. et al. (1999) J Biol. Chem. 274(39):27605.
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 Research Articles on WASF1    1. WAVE1 might be involved in the migration and invasion of K562 cells through regulation of the expression level of MMP-2.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-WASF1 antibodyPathways associated with anti-WASF1 antibody
 Reference Product  PubMed Publications
 ACTR2 antibody  >13 publications with WASF1 and ACTR2
 CDC42 antibody  >12 publications with WASF1 and CDC42
 RAC1 antibody  >6 publications with WASF1 and RAC1
 ABI1 antibody  >5 publications with WASF1 and ABI1
 PFN1 antibody  >2 publications with WASF1 and PFN1
 CDK5 antibody  >2 publications with WASF1 and CDK5
 Products by Pathway  Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 E2F Transcription Factor Network Pathway antibodies  E2F Transcription Factor Network Pathway Diagram
 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram
 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram
 RAC1 Signaling Pathway antibodies  RAC1 Signaling Pathway Diagram
 Regulation Of Actin Cytoskeleton Pathway antibodies  Regulation Of Actin Cytoskeleton Pathway Diagram
 Regulation Of Actin Cytoskeleton Pathway antibodies  Regulation Of Actin Cytoskeleton Pathway Diagram
Diseases associated with anti-WASF1 antibodyOrgans/Tissues associated with anti-WASF1 antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >12 publications with WASF1 and Neoplasms
 Blood Coagulation Disorders Antibodies  >6 publications with WASF1 and Blood Coagulation Disorders
 Neurodegenerative Diseases Antibodies  >2 publications with WASF1 and Neurodegenerative Diseases
 Muscular Diseases Antibodies  >1 publications with WASF1 and Muscular Diseases
 Melanoma, Experimental Antibodies  >1 publications with WASF1 and Melanoma, Experimental
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >14 publications with WASF1 and Brain
 Blood Antibodies  >7 publications with WASF1 and Blood
 Nerve Antibodies  >6 publications with WASF1 and Nerve
 Muscle Antibodies  >4 publications with WASF1 and Muscle
 Bone Antibodies  >2 publications with WASF1 and Bone
 Stomach Antibodies  >1 publications with WASF1 and Stomach
 Intestine Antibodies  >1 publications with WASF1 and Intestine
 Skin Antibodies  >1 publications with WASF1 and Skin
 Lung Antibodies  >1 publications with WASF1 and Lung
 Kidney Antibodies  >1 publications with WASF1 and Kidney
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