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anti-WEE1 antibody :: Rabbit WEE1 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS9407093
Western Blot (WB)
Unit / Price
0.05 mL  /  $200 +1 FREE 8GB USB
0.1 mL  /  $255 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

WEE1, Polyclonal Antibody

 Also Known As   

WEE1 (Phospho-Ser642) Antibody

 Product Synonym Names    WEE1A; WEE1hu; kinase WEE1
 Product Gene Name   

anti-WEE1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    193525
 3D Structure    ModBase 3D Structure for P30291
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 Clonality    Polyclonal
 Host    Rabbit
 Species Reactivity    Human, Mouse, Rat
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 Specificity    The antibody detects endogenous levels of WEE1 only when phosphorylated at serine 642.
 Purity/Purification    Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
 Form/Format    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
 Concentration    1.0 mg/ml (lot specific)
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 Immunogen Type    Peptide-KLH
 Immunogen Description    Peptide sequence around phosphorylation site of Serine 642(S-V-S(p)-L-T) derived from Human WEE1.
 Modification    Phospho-Ser642
 Target Name    WEE1
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 Preparation and Storage    Store at -20 degree C/1 year
 Other Notes    Small volumes of anti-WEE1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-WEE1 antibody

   Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.Watanabe N., EMBO J. 14:1878-1891(1995).Cichutek A., Cytogenet. Cell Genet. 93:277-283(2001).Igarashi M., Nature 353:80-83(1991)
 Product Categories/Family for anti-WEE1 antibody    Phospho-specific Ab
 Applications Tested/Suitable for anti-WEE1 antibody   

Western Blot (WB)

 Application Notes for anti-WEE1 antibody    Western blotting: 1:500~1:1000
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 Western Blot (WB) of anti-WEE1 antibody    Western blot analysis of extracts from 293 cells treated with etoposide using WEE1 (Phospho-Ser642) Antibody.The lane on the right is treated with the antigen-specific peptide.
anti-WEE1 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for WEE1. It may not necessarily be applicable to this product.
 NCBI GI #    221307502
 NCBI GeneID    7465
 NCBI Accession #    NP_001137448.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001143976.1 [Other Products]
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 UniProt Primary Accession #    P30291 [Other Products]
 UniProt Secondary Accession #    B3KVE1; D3DQV0 [Other Products]
 UniProt Related Accession #    P30291 [Other Products]
 Molecular Weight    49,072 Da
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 NCBI Official Full Name    wee1-like protein kinase isoform 2
 NCBI Official Synonym Full Names    WEE1 G2 checkpoint kinase
 NCBI Official Symbol    WEE1 [Similar Products]
 NCBI Official Synonym Symbols   
WEE1A; WEE1hu
[Similar Products]
 NCBI Protein Information    wee1-like protein kinase
 UniProt Protein Name    Wee1-like protein kinase
 UniProt Synonym Protein Names   
Wee1A kinase
 Protein Family    Wee1-like protein kinase
 UniProt Gene Name    WEE1 [Similar Products]
 UniProt Synonym Gene Names    WEE1hu [Similar Products]
 UniProt Entry Name    WEE1_HUMAN
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 NCBI Summary for WEE1    This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. [provided by RefSeq, Jul 2008]
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 UniProt Comments for WEE1    Wee1: a protein kinase of the WEE family. A nuclear protein which catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase.

Protein type: Protein kinase, Other; EC 2.7.10.2; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Other group; WEE family

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; magnesium ion binding; ATP binding

Biological Process: mitosis; peptidyl-tyrosine phosphorylation; establishment of cell polarity; regulation of cell cycle; cell division; microtubule cytoskeleton organization and biogenesis; mitotic cell cycle; blood coagulation; G2/M transition of mitotic cell cycle; neurite morphogenesis; G1/S transition of mitotic cell cycle
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 Research Articles on WEE1    1. WEE1 is a valid target of the miR-17-92 cluster in leukemia.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-WEE1 antibodyPathways associated with anti-WEE1 antibody
 Reference Product  PubMed Publications
 CDK1 antibody  >166 publications with WEE1 and CDK1
 CCNB1 antibody  >86 publications with WEE1 and CCNB1
 CDC25C antibody  >69 publications with WEE1 and CDC25C
 CDK2 antibody  >47 publications with WEE1 and CDK2
 PLK1 antibody  >26 publications with WEE1 and PLK1
 CDC25A antibody  >19 publications with WEE1 and CDC25A
 CDC25B antibody  >17 publications with WEE1 and CDC25B
 HSP90AA1 antibody  >1 publications with WEE1 and HSP90AA1
 Products by Pathway  Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Checkpoints Pathway antibodies  Cell Cycle Checkpoints Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle - G2/M Transition Pathway antibodies  Cell Cycle - G2/M Transition Pathway Diagram
 Cell Cycle - G2/M Transition Pathway antibodies  Cell Cycle - G2/M Transition Pathway Diagram
 Chk1/Chk2(Cds1) Mediated Inactivation Of Cyclin B:Cdk1 Complex Pathway antibodies  Chk1/Chk2(Cds1) Mediated Inactivation Of Cyclin B:Cdk1 Complex Pathway Diagram
 Cyclin A/B1 Associated Events During G2/M Transition Pathway antibodies  Cyclin A/B1 Associated Events During G2/M Transition Pathway Diagram
Diseases associated with anti-WEE1 antibodyOrgans/Tissues associated with anti-WEE1 antibody
 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >24 publications with WEE1 and Breast Neoplasms
 Neoplasms, Experimental Antibodies  >16 publications with WEE1 and Neoplasms, Experimental
 Liver Diseases Antibodies  >15 publications with WEE1 and Liver Diseases
 Brain Diseases Antibodies  >13 publications with WEE1 and Brain Diseases
 Carcinoma, Squamous Cell Antibodies  >13 publications with WEE1 and Carcinoma, Squamous Cell
 Liver Neoplasms Antibodies  >10 publications with WEE1 and Liver Neoplasms
 Lung Neoplasms Antibodies  >10 publications with WEE1 and Lung Neoplasms
 Carcinoma, Hepatocellular Antibodies  >9 publications with WEE1 and Carcinoma, Hepatocellular
 Cell Transformation, Neoplastic Antibodies  >8 publications with WEE1 and Cell Transformation, Neoplastic
 Pancreatic Neoplasms Antibodies  >6 publications with WEE1 and Pancreatic Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >26 publications with WEE1 and Liver
 Brain Antibodies  >24 publications with WEE1 and Brain
 Blood Antibodies  >22 publications with WEE1 and Blood
 Lung Antibodies  >17 publications with WEE1 and Lung
 Prostate Antibodies  >16 publications with WEE1 and Prostate
 Kidney Antibodies  >14 publications with WEE1 and Kidney
 Bone Antibodies  >11 publications with WEE1 and Bone
 Ovary Antibodies  >9 publications with WEE1 and Ovary
 Embryonic Tissue Antibodies  >8 publications with WEE1 and Embryonic Tissue
 Vascular Antibodies  >8 publications with WEE1 and Vascular
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