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anti-WNT3 antibody :: Goat WNT3 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS421042
Unit / Price
0.1 mg  /  $300 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

WNT3, Polyclonal Antibody

 Also Known As   

Goat anti-WNT3 Antibody

 Product Synonym Names    WNT3; wingless-type MMTV integration site family, member 3; INT4; MGC131950; MGC138321; MGC138323; WNT-3 proto-oncogene protein precursor; WNT3 antibody; wingless-type MMTV integration site family; member 3 antibody; INT4 antibody; MGC131950 antibody; MGC138321 antibody; MGC138323 antibody; WNT-3 proto-oncogene protein precursor antibody
 Product Gene Name   

anti-WNT3 antibody

[Similar Products]
 Antibody/Peptide Pairs    WNT3 peptide (MBS425179) is used for blocking the activity of WNT3 antibody (MBS421042)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence    CGRGHNTRTE KRKEK
 OMIM    165330
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 Clonality    Polyclonal
 Host    Goat
 Species Reactivity    Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog
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 Purity/Purification    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
 Form/Format    Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
 Concentration    100ug specific antibody in 200ul (lot specific)
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 Immunogen    Peptide with sequence CGRGHNTRTEKRKEK, from the internal region of the protein sequence according to NP_110380.1.
 Epitope    Internal region
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 Preparation and Storage    Aliquot and store at -20 degree C. Minimize freezing and thawing.
 ISO Certification    Manufactured in an ISO 9001:2015 Certified Laboratory.
 Other Notes    Small volumes of anti-WNT3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-WNT3 antibody   

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

 Application Notes for anti-WNT3 antibody    Peptide ELISA: Antibody detection limit dilution 1: 16000.
Immunohistochemistry: In paraffin embedded Human Breast shows cytoplasm staining in some luminal epithelial cells. Recommended concentration, 2-4ug/ml.
Western Blot: Approx 40kDa band observed in lysates of cell line HEK293 (calculated MW of 39.6kDa according to NP_110380.1). Recommended concentration: 1-3ug/ml.
Immunocytochemsitry/Immunofluorescence This antibody has been successfully used in ICC/IF for mouse hippocampal primary astrocytes as described in the following paper: Okamoto et al, FASEB J.2011 Jul 11; PMID: 21746862.
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 Western Blot (WB) of anti-WNT3 antibody    (1ug/ml) staining of lysate of cell line HEK293 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-WNT3 antibody Western Blot (WB) (WB) image
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 Immunohistochemistry (IHC) of anti-WNT3 antibody    (2ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
anti-WNT3 antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for WNT3. It may not necessarily be applicable to this product.
 NCBI GI #    13540477
 NCBI GeneID    7473
 NCBI Accession #    NP_110380.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_030753.4 [Other Products]
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 UniProt Secondary Accession #    Q2M237; Q9H1J9 [Other Products]
 UniProt Related Accession #    P56703 [Other Products]
 Molecular Weight    39,645 Da
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 NCBI Official Full Name    proto-oncogene Wnt-3
 NCBI Official Synonym Full Names    Wnt family member 3
 NCBI Official Symbol    WNT3 [Similar Products]
 NCBI Official Synonym Symbols   
INT4; TETAMS
[Similar Products]
 NCBI Protein Information    proto-oncogene Wnt-3
 UniProt Protein Name    Proto-oncogene Wnt-3
 UniProt Synonym Protein Names   
Proto-oncogene Int-4 homolog
 Protein Family    Proto-oncogene
 UniProt Gene Name    WNT3 [Similar Products]
 UniProt Synonym Gene Names    INT4 [Similar Products]
 UniProt Entry Name    WNT3_HUMAN
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 NCBI Summary for WNT3    The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98% amino acid identity to mouse Wnt3 protein, and 84% to human WNT3A protein, another WNT gene product. The mouse studies show the requirement of Wnt3 in primary axis formation in the mouse. Studies of the gene expression suggest that this gene may play a key role in some cases of human breast, rectal, lung, and gastric cancer through activation of the WNT-beta-catenin-TCF signaling pathway. This gene is clustered with WNT15, another family member, in the chromosome 17q21 region. [provided by RefSeq, Jul 2008]
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 UniProt Comments for WNT3    WNT3: Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. Defects in WNT3 are the cause of autosomal recessive tetra-amelia (ARTTRA). Tetra-amelia is a rare human genetic disorder characterized by complete absence of all four limbs and other anomalies such as craniofacial, nervous system, pulmonary, skeletal and urogenital defects. Belongs to the Wnt family.

Protein type: Secreted; Oncoprotein; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: endoplasmic reticulum lumen; extracellular region; extracellular space; Golgi lumen; plasma membrane; proteinaceous extracellular matrix

Molecular Function: frizzled binding; protein binding; protein domain specific binding; receptor agonist activity

Biological Process: anterior/posterior axis specification; axon guidance; cell fate commitment; cell morphogenesis; dorsal/ventral axis specification; embryonic forelimb morphogenesis; embryonic hindlimb morphogenesis; gamete generation; limb bud formation; mesoderm formation; negative regulation of axon extension involved in axon guidance; neuron differentiation; positive regulation of collateral sprouting in the absence of injury; positive regulation of Wnt receptor signaling pathway; Spemann organizer formation at the anterior end of the primitive streak; Wnt receptor signaling pathway; Wnt receptor signaling pathway through beta-catenin

Disease: Tetraamelia, Autosomal Recessive
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 Research Articles on WNT3    1. Genome association study shows a highly conserved 32 kb intergenic region containing regulatory elements between WNT3 and WNT9B in patients with classic bladder exstrophy.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-WNT3 antibodyPathways associated with anti-WNT3 antibody
 Reference Product  PubMed Publications
 LRP6 antibody  >41 publications with WNT3 and LRP6
 BMP4 antibody  >37 publications with WNT3 and BMP4
 FZD1 antibody  >21 publications with WNT3 and FZD1
 FZD7 antibody  >19 publications with WNT3 and FZD7
 FZD4 antibody  >12 publications with WNT3 and FZD4
 SFRP2 antibody  >9 publications with WNT3 and SFRP2
 WLS antibody  >9 publications with WNT3 and WLS
 FZD5 antibody  >9 publications with WNT3 and FZD5
 RYK antibody  >9 publications with WNT3 and RYK
 FZD8 antibody  >6 publications with WNT3 and FZD8
 Products by Pathway  Pathway Diagram
 Basal Cell Carcinoma Pathway antibodies  Basal Cell Carcinoma Pathway Diagram
 Basal Cell Carcinoma Pathway antibodies  Basal Cell Carcinoma Pathway Diagram
 Class B/2 (Secretin Family Receptors) Pathway antibodies  Class B/2 (Secretin Family Receptors) Pathway Diagram
 DNA Damage Response (only ATM Dependent) Pathway antibodies  DNA Damage Response (only ATM Dependent) Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 Hedgehog Signaling Pathway antibodies  Hedgehog Signaling Pathway Diagram
 Hedgehog Signaling Pathway antibodies  Hedgehog Signaling Pathway Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram
Diseases associated with anti-WNT3 antibodyOrgans/Tissues associated with anti-WNT3 antibody
 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >38 publications with WNT3 and Disease Models, Animal
 Breast Neoplasms Antibodies  >26 publications with WNT3 and Breast Neoplasms
 Nervous System Diseases Antibodies  >26 publications with WNT3 and Nervous System Diseases
 Embryo Loss Antibodies  >23 publications with WNT3 and Embryo Loss
 Adenocarcinoma Antibodies  >20 publications with WNT3 and Adenocarcinoma
 Brain Diseases Antibodies  >16 publications with WNT3 and Brain Diseases
 Neoplasms, Experimental Antibodies  >14 publications with WNT3 and Neoplasms, Experimental
 Necrosis Antibodies  >14 publications with WNT3 and Necrosis
 Fibrosis Antibodies  >11 publications with WNT3 and Fibrosis
 Inflammation Antibodies  >10 publications with WNT3 and Inflammation
 Organ/Tissue Name  Pubmed Publications
 Embryonic Tissue Antibodies  >114 publications with WNT3 and Embryonic Tissue
 Brain Antibodies  >97 publications with WNT3 and Brain
 Heart Antibodies  >42 publications with WNT3 and Heart
 Skin Antibodies  >36 publications with WNT3 and Skin
 Kidney Antibodies  >26 publications with WNT3 and Kidney
 Mammary Gland Antibodies  >16 publications with WNT3 and Mammary Gland
 Stomach Antibodies  >7 publications with WNT3 and Stomach
 Testis Antibodies  >6 publications with WNT3 and Testis
 Parathyroid Antibodies  >4 publications with WNT3 and Parathyroid
 Uncharacterized Tissue Antibodies  >2 publications with WNT3 and Uncharacterized Tissue
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