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anti-WRNIP1 antibody :: Rabbit WRNIP1 / WHIP Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS240498
Unit / Price
0.05 mg  /  $495 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

WRNIP1 / WHIP, Polyclonal Antibody

 Also Known As   

Rabbit Polyclonal to Human WRNIP1 / WHIP

 Product Synonym Names    Anti-WRNIP1 / WHIP Antibody (Internal) IHC-plus; WRNIP1; Putative helicase RUVBL; WHIP; RP11-420G6.2; ATPase WRNIP1; BA420G6.2; Human WRNIP1; WHIP
 Product Gene Name   

anti-WRNIP1 antibody

[Similar Products]
 Product Synonym Gene Name    WHIP [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    608196
 3D Structure    ModBase 3D Structure for Q96S55
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 Clonality    Polyclonal
 Host    Rabbit
 Species Reactivity    Human, Monkey, Mouse, Rat
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 Specificity    An internal region of the WHIP1 protein. The immunogen sequence shows 100% homology to human WHIP1 (isoform 1) and WHIP2 (isoform 2) with predicted molecular weights of 72.2 kD and 69.5 kD, respectively. The immunogen sequence also shows 100% homology to WHIP1 from mouse, rat and monkey sequences. Reactivity with WHIP proteins from other sources is not known, but is likely due to reported homologies.
 Purity/Purification    Immunoaffinity Purified
 Form/Format    0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
 Concentration    1 mg/ml (lot specific)
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 Target Species    Human
 Immunogen Description    Synthetic peptide from human WRNIP1 / WHIP.
 Immunogen Type    Synthetic peptide
 Immunogen    WRNIP1 / WHIP antibody was raised against synthetic peptide from human WRNIP1 / WHIP.
 Antigen Modification    Internal
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 Preparation and Storage    +4 degree C or -20 degree C, Avoid repeated freezing and thawing.
 Other Notes    Small volumes of anti-WRNIP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-WRNIP1 antibody   

Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)

 Application Notes for anti-WRNIP1 antibody    ELISA (1:10000 - 1:40000), IHC-P (2.5 ug/ml), WB (1:500 - 1:2000)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...
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 Immunohistochemistry (IHC) of anti-WRNIP1 antibody    Anti-WRNIP1 / WHIP antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
anti-WRNIP1 antibody Immunohistochemistry (IHC) (IHC) image
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 Western Blot (WB) of anti-WRNIP1 antibody    Anti-Human WHIP Antibody - Western Blot. Western blot analysis is shown using Affinity Purified anti-Human WHIP antibody to detect Human WHIP present in a HEK293 whole cell lysate. ~30 ug of lysate was loaded per lane for 4-20% gradient SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a primary band of ~96.0 kD is detected. The identity of the minor band migrating at a slightly higher molecular weight is unknown, but may represent an alternate isoform of WHIP or post translational modification of the WHIP protein. See Figure 2 for the results of peptide competition experiments. The blot was incubated with a 1:200 dilution of the antibody at room temperature for 2 h followed by detection using IRDye 800 labeled Goat-a-Rabbit IgG [H&L] MX10 (diluted 1:5000 for 45 min. IRDye 800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
anti-WRNIP1 antibody Western Blot (WB) (WB) image
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 Western Blot (WB) of anti-WRNIP1 antibody    Anti-Human WHIP Antibody - Western Blot. Western blot analysis is shown using anti-Human WHIP antibody with and without pre-incubation with blocking peptide. Testing was performed on antiserum prior to affinity purification. Peptide competition (left) blocks the specific staining, whereas the control (right) shows staining of a strong dominant band corresponding to human WHIP1. ~30 ug of HEK293 lysate was loaded per lane for 4-20% gradient SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a band of ~96.0 kD is detected. The blot was incubated with a 1:1000 dilution of the antibody at room temperature for 2 h followed by detection using IRDye 800 labeled Goat-a-Rabbit IgG [H&L] MX10 (diluted 1:5000 for 45 min. IRDye 800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other systems will yield similar results.
anti-WRNIP1 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for WRNIP1. It may not necessarily be applicable to this product.
 NCBI GI #    18426902
 NCBI GeneID    56897
 NCBI Accession #    NP_064520.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_020135.2 [Other Products]
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 UniProt Primary Accession #    Q96S55 [Other Products]
 UniProt Secondary Accession #    Q53EP6; Q59ET8; Q5W0E2; Q5W0E4; Q8WV26; Q9H681; Q9NRJ6; B2RDB0 [Other Products]
 UniProt Related Accession #    Q96S55 [Other Products]
 Molecular Weight    31,339 Da
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 NCBI Official Full Name    ATPase WRNIP1 isoform 1
 NCBI Official Synonym Full Names    Werner helicase interacting protein 1
 NCBI Official Symbol    WRNIP1 [Similar Products]
 NCBI Official Synonym Symbols   
WHIP; bA420G6.2
[Similar Products]
 NCBI Protein Information    ATPase WRNIP1; putative helicase RUVBL; Werner helicase-interacting protein 1
 UniProt Protein Name    ATPase WRNIP1
 UniProt Synonym Protein Names   
Werner helicase-interacting protein 1
 Protein Family    ATPase
 UniProt Gene Name    WRNIP1 [Similar Products]
 UniProt Entry Name    WRIP1_HUMAN
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 NCBI Summary for WRNIP1    Werner's syndrome is a rare autosomal recessive disorder characterized by accelerated aging that is caused by defects in the Werner syndrome ATP-dependent helicase gene (WRN). The protein encoded by this gene interacts with the exonuclease-containing N-terminal portion of the Werner protein. This protein has a ubiquitin-binding zinc-finger domain in the N-terminus, an ATPase domain, and two leucine zipper motifs in the C-terminus. It has sequence similarity to replication factor C family proteins and is conserved from E. coli to human. This protein likely accumulates at sites of DNA damage by interacting with polyubiquinated proteins and also binds to DNA polymerase delta and increases the initiation frequency of DNA polymerase delta-mediated DNA synthesis. This protein also interacts with nucleoporins at nuclear pore complexes. Two transcript variants encoding different isoforms have been isolated for this gene. [provided by RefSeq, Jul 2012]
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 UniProt Comments for WRNIP1    WRNIP1: a modulator for initiation or restart of DNA polymerase delta-mediated DNA synthesis. Its ATPase activity may sense DNA ends and regulate the extent of polymerase stimulation.

Protein type: Helicase; EC 3.6.1.3

Chromosomal Location of Human Ortholog: 6p25.2

Cellular Component: membrane; perinuclear region of cytoplasm; nucleus

Molecular Function: identical protein binding; protein binding; DNA binding; metal ion binding; ATPase activity; ATP binding

Biological Process: regulation of DNA replication initiation; DNA synthesis during DNA repair; DNA replication
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 Research Articles on WRNIP1    1. results suggest that WRNIP1 functions upstream of Poleta in the response to UV irradiation
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products related to Rabbit Polyclonal to Human WRNIP1 / WHIP
 Catalog #  Product Name
 MBS240498  Rabbit Polyclonal to Human WRNIP1 / WHIP
Products associated with anti-WRNIP1 antibodyDiseases associated with anti-WRNIP1 antibody
 Reference Product  PubMed Publications
 WRN antibody  >10 publications with WRNIP1 and WRN
 RAD18 antibody  >3 publications with WRNIP1 and RAD18
 PCNA antibody  >3 publications with WRNIP1 and PCNA
 Disease Name  Pubmed Publications
 Neurologic Manifestations Antibodies  >1 publications with WRNIP1 and Neurologic Manifestations
 Urogenital Abnormalities Antibodies  >1 publications with WRNIP1 and Urogenital Abnormalities
 Neurobehavioral Manifestations Antibodies  >1 publications with WRNIP1 and Neurobehavioral Manifestations
 Inflammation Antibodies  >1 publications with WRNIP1 and Inflammation
Organs/Tissues associated with anti-WRNIP1 antibody
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >2 publications with WRNIP1 and Blood
 Larynx Antibodies  >1 publications with WRNIP1 and Larynx
 Lung Antibodies  >1 publications with WRNIP1 and Lung
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