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| Product Name
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ZFP281 (ZNF281), Polyclonal Antibody
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| Also Known As
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ZFP281 Antibody
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| Product Synonym Names
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ZFP281; ZBP-99; ZNP-99; GZP1; ZBP99; Zinc finger protein 281; GC-box-binding zinc finger protein 1; zinc finger protein 281
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| Product Gene Name
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[Similar Products]
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| Antibody/Peptide Pairs
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ZFP281 peptide (MBS151960) is used for blocking the activity of ZFP281 antibody (MBS151071)
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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 TOP
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| 3D Structure
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ModBase 3D Structure for Q9Y2X9
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| Clonality
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Polyclonal
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| Isotype
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IgG
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| Host
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Rabbit
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| Species Reactivity
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Human, Mouse
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| Purity/Purification
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ZFP281 Antibody is affinity chromatography purified via peptide column.
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| Form/Format
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Liquid
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| Concentration
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1 mg/mL (lot specific)
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| Conjugate
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Unconjugated
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| Immunogen
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ZFP281 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human ZFP281.
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| Buffer
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ZFP281 Antibody is supplied in PBS containing 0.02% sodium azide.
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| Preparation and Storage
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ZFP281 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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| Other Notes
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Small volumes of anti-ZNF281 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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TOP
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ZFP281 Antibody: ZFP281, also known as ZBP-99, is a member of a conserved family of transcription factors that interact with GC-rich promoters. It contains four Kruppel-like zinc fingers and is highly homologous to ZBP-89, another regulator of ornithine decarboxylase gene expression. ZFP281 also interacts with many other other transcription factors, such as Sp1 to repress vimentin gene transcription. ZFP281 has also been shown to control embryonic stem cell pluripotency by direct regulating specific target genes. ZFP281 phyically interacts with the transcription factos POU5F1, Sox2, and NANOG. Gene expression microarray anlysis indicated that some ZFP281 target genes were activated, while others were repressed upon knockdown of ZFP281 expression, showing that ZFP281 plays bifuctinal roles in regulating gene expression in stem cells.
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| Applications Tested/Suitable for anti-ZNF281 antibody
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ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
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| Application Notes for anti-ZNF281 antibody
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ZFP281 antibody can be used for detection of ZFP281 by Western blot at 0.25 - 0.5 mug/mL. Antibody can also be used for immunofluorescence starting at 20 mug/mL. For immunofluorescence start at 20 mug/mL.
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| Western Blot (WB) of anti-ZNF281 antibody
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Western blot analysis of ZFP281 in A-20 cell lysate with ZFP281 antibody at (A) 0.25 and (B) 0.5 μg/mL.
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| Immunofluorescence (IF) of anti-ZNF281 antibody
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Immunofluorescence of ZFP291 in A20 cells with ZFP291 antibody at 20 μg/mL.
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TOP
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| NCBI/Uniprot data below describe general gene information for ZNF281. It may not necessarily be applicable to this product. |
| NCBI GI #
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6912752
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| NCBI GeneID
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23528
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| NCBI Accession #
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AAH51905
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[Other Products]
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| NCBI GenBank Nucleotide #
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NM_012482.4
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[Other Products]
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| UniProt Primary Accession #
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Q9Y2X9
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[Other Products]
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| UniProt Secondary Accession #
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Q5RKW5; Q9NY92; A6NF48; B3KMX2
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[Other Products]
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| UniProt Related Accession #
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Q9Y2X9
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[Other Products]
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| Molecular Weight
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93,232 Da
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TOP
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| NCBI Official Full Name
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zinc finger protein 281 isoform 1
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| NCBI Official Synonym Full Names
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zinc finger protein 281
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| NCBI Official Symbol
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ZNF281
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[Similar Products]
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| NCBI Official Synonym Symbols
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ZBP-99; ZNP-99
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[Similar Products]
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| NCBI Protein Information
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zinc finger protein 281; ZNP-99 transcription factor; transcription factor ZBP-99; zinc finger DNA-binding protein 99; GC-box-binding zinc finger protein 1
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| UniProt Protein Name
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Zinc finger protein 281
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| UniProt Synonym Protein Names
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GC-box-binding zinc finger protein 1; Transcription factor ZBP-99; Zinc finger DNA-binding protein 99
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| Protein Family
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Zinc finger protein
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| UniProt Gene Name
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ZNF281
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[Similar Products]
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| UniProt Synonym Gene Names
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GZP1; ZBP99
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[Similar Products]
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| UniProt Entry Name
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ZN281_HUMAN
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| UniProt Comments for ZNF281
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ZNF281: Involved in transcriptional regulation. Represses the transcription of a number of genes including gastrin and ornithine decarboxylase. Binds to the G-rich box in the enhancer region of these genes. Belongs to the krueppel C2H2-type zinc-finger protein family.
Protein type: DNA-binding; C2H2-type zinc finger protein; Transcription factor
Chromosomal Location of Human Ortholog: 1q32.1
Cellular Component: nucleoplasm; nucleus
Molecular Function: protein binding; sequence-specific DNA binding; metal ion binding; transcription factor activity; transcription corepressor activity
Biological Process: embryonic body morphogenesis; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; stem cell differentiation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent
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TOP
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| Research Articles on ZNF281
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1. High ZNF281 expression is associated with colon and breast cancer.
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TOP
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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| Products associated with anti-ZNF281 antibody | Diseases associated with anti-ZNF281 antibody |
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| Organs/Tissues associated with anti-ZNF281 antibody |
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Antibody 
