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anti-ZNF148 antibody :: Rabbit ZNF148 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS9600566
Unit / Price
0.1 mL  /  $255 +1 FREE 8GB USB
0.2 mL  /  $295 +1 FREE 8GB USB
 
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 Product Name   

ZNF148, Polyclonal Antibody

★Popular Item★
 Also Known As   

ZNF148 Antibody

 Product Synonym Names    Transcription factor ZBP-89; Transcription factor ZBP89; ZBP89; Zinc finger DNA binding protein 89; Zinc finger DNA-binding protein 89; Zinc finger protein 148; ZN148_HUMAN; ZNF 148; ZNF148
 Product Gene Name   

anti-ZNF148 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 3D Structure    ModBase 3D Structure for Q9UQR1
 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human, Mouse, Rat
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 Specificity    ZNF148 antibody detects endogenous levels of ZNF148
 Purity/Purification    The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
 Form/Format    Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
 Concentration    1mg/ml (lot specific)
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 Immunogen    A synthesized peptide derived from human ZNF148
 Subcellular Location    Nucleus.
 Conjugation    Unconjugated
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 Preparation and Storage    Store at -20 degree C. Stable for 12 months from date of receipt.
 Other Notes    Small volumes of anti-ZNF148 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-ZNF148 antibody

   Description: ZNF148 a transcription factor. Represses the transcription of a number of genes including gastrin, stromelysin and enolase. Binds to the G-rich box in the enhancer region of these genes. Belongs to the krueppel C2H2-type zinc-finger protein family.
Function: Involved in transcriptional regulation. Represses the transcription of a number of genes including gastrin, stromelysin and enolase. Binds to the G-rich box in the enhancer region of these genes.
Subunit Structure: Interacts with HNRNPDL (PubMed:15190078). Interacts with the 5FMC complex; the interaction requires association with CHTOP. Interacts with CAVIN1 (By similarity).
Post-translational Modifications: Sumoylated with SUMO2. Desumoylated by SENP3, resulting in the stimulation of transcription of its target genes (By similarity).
Similarity: Belongs to the krueppel C2H2-type zinc-finger protein family.
 Applications Tested/Suitable for anti-ZNF148 antibody   

Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)

 Application Notes for anti-ZNF148 antibody    WB: 1:500-1:2000
IHC: 1:50-1:200
IF/ICC: 1:100-1:500
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 Immunofluorescence (IF) of anti-ZNF148 antibody    MBS9600566 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
anti-ZNF148 antibody Immunofluorescence (IF) image
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 Western Blot (WB) of anti-ZNF148 antibody    Western blot analysis on HepG2 cell lysate using ZNF148 Antibody. The lane on the left is treated with the antigen-specific peptide.
anti-ZNF148 antibody Western Blot (WB) (WB) image
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 Western Blot (WB) of anti-ZNF148 antibody    Western blot analysis of extracts from various samples, using ZNF148 Antibody. Lane 1: rat brain treated with blocking peptide. Lane 2: rat brain; Lane 3: mouse brain;
anti-ZNF148 antibody Western Blot (WB) (WB) image
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 Immunohistochemistry (IHC) of anti-ZNF148 antibody    MBS9600566 at 1/100 staining human Kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
anti-ZNF148 antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for ZNF148. It may not necessarily be applicable to this product.
 NCBI GI #    1143076908
 NCBI GeneID    7707
 NCBI Accession #    NP_001335353.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001348424.1 [Other Products]
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 UniProt Primary Accession #    Q9UQR1 [Other Products]
 UniProt Secondary Accession #    O00389; O43591; Q58EY5; Q6PJ98; D3DN27 [Other Products]
 UniProt Related Accession #    Q9UQR1 [Other Products]
 Molecular Weight    Observed: 89 kDa
Predicted: 89 kDa
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 NCBI Official Full Name    zinc finger protein 148 isoform a
 NCBI Official Synonym Full Names    zinc finger protein 148
 NCBI Official Symbol    ZNF148 [Similar Products]
 NCBI Official Synonym Symbols   
BERF-1; BFCOL1; GDACCF; ZBP-89; ZFP148; pHZ-52; HT-BETA
[Similar Products]
 NCBI Protein Information    zinc finger protein 148
 UniProt Protein Name    Zinc finger protein 148
 UniProt Synonym Protein Names   
Transcription factor ZBP-89; Zinc finger DNA-binding protein 89
 Protein Family    Zinc finger protein
 UniProt Gene Name    ZNF148 [Similar Products]
 UniProt Synonym Gene Names    ZBP89 [Similar Products]
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 NCBI Summary for ZNF148    The protein encoded by this gene is a member of the Kruppel family of zinc finger DNA binding proteins. The encoded protein activates transcription of the T-cell receptor and intestinal alkaline phosphatase genes but represses transcription of the ornithine decarboxylase, vimentin, gastrin, stomelysin, and enolase genes. Increased expression of this gene results in decreased patient survival rates from colorectal cancer, while mutations in this gene have been associated with global developmental delay, hypoplastic corpus callosum, and dysmorphic facies. [provided by RefSeq, Feb 2017]
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 UniProt Comments for ZNF148    Involved in transcriptional regulation. Represses the transcription of a number of genes including gastrin, stromelysin and enolase. Binds to the G-rich box in the enhancer region of these genes.
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 Research Articles on ZNF148    1. ZNF148 as a gene involved in a newly described intellectual disability syndrome with a recurrent phenotype and postulate that the ZNF148 is a hitherto unrecognized but crucial transcription factor in the development of the corpus callosum.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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