View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List Rabbit anti-Human ZO1 Polyclonal Antibody Catalog # MBS645406 Unit / Price 0.2 mL / $770 +1 FREE 8GB USB 5x0.2 mL / $3,295 +4 FREE 8GB USB Previous Next Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name ZO1, Polyclonal Antibody Full Product Name ZO1, CT (TJP1, ZO1, Tight junction protein ZO-1, Tight junction protein 1, Zona occludens protein 1, Zonula occludens protein 1) Product Synonym Names Anti -ZO1, CT (TJP1, ZO1, Tight junction protein ZO-1, Tight junction protein 1, Zona occludens protein 1, Zonula occludens protein 1) Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Clonality Polyclonal Isotype IgG Host Rabbit Species Reactivity Human Specificity Human Purity/Purification PurifiedPurified by saturated ammonium sulfate precipitation followed by dialysis against PBS. Form/Format Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Immunogen ZO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1578~1607 amino acids from the C-terminal region of human ZO1. Preparation and Storage May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Other Notes Small volumes of anti-ZO1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for anti-ZO1 antibody ZO1 encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Product Categories/Family for anti-ZO1 antibody Antibodies; Abs to Connexin; Junction Proteins Applications Tested/Suitable for anti-ZO1 antibody ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS) Application Notes for anti-ZO1 antibody Suitable for use in Western Blot, Immunohistochemistry, Flow CytometryDilution: ELISA: 1:1,000Western Blot: 1:50-100Immunohistochemistry: 1:50-100Flow Cytometry: 1:10-50 Western Blot (WB) of anti-ZO1 antibody Western blot analysis of ZO1 Antibody (C-term) in K562 cell line lysates (35ug/lane). ZO1 (arrow) was detected using the purified Pab. Immunohistochemistry (IHC) of anti-ZO1 antibody ZO1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ZO1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. Flow Cytometry (FC/FACS) of anti-ZO1 antibody ZO1 Antibody (C-term) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP