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anti-ACTN4 antibody :: Rabbit anti-Human alpha Actinin 4 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS9402613
Unit / Price
0.1 mL  /  $320 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

alpha Actinin 4 (ACTN4), Polyclonal Antibody

 Also Known As   

alpha Actinin 4 antibody

 Product Gene Name   

anti-ACTN4 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    603278
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 Clonality    Polyclonal
 Host    Rabbit
 Species Reactivity    Human
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 Purity/Purification    Purified by antigen-affinity chromatography.
 Form/Format    Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
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 Immunogen Type    Recombinant protein
 Immunogen Description    Recombinant protein fragment contain a sequence corresponding to a region within amino acids 206 and 512 of alpha Actinin 4
 Target Name    alpha Actinin 4
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 Preparation and Storage    Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.
 Other Notes    Small volumes of anti-ACTN4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-ACTN4 antibody

   Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq]
 Product Categories/Family for anti-ACTN4 antibody    Total protein Ab
 Applications Tested/Suitable for anti-ACTN4 antibody   

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

 Application Notes for anti-ACTN4 antibody    Western blotting: 1:500-1:3000
Immunohistochemistry: 1:100-1:500
Immunofluorescence: 1:100-1:200
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 Testing Data of anti-ACTN4 antibody    Sample (30 ug of whole cell lysate) A: A431 B: Hep G2 7.5% SDS PAGE Primary antibody diluted at 1: 1000
anti-ACTN4 antibody Testing Data image
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 Immunohistochemistry (IHC) of anti-ACTN4 antibody    Immunohistochemical analysis of paraffin-embedded A549 xenograft, using alpha Actinin 4 antibody at 1: 500 dilution.
anti-ACTN4 antibody Immunohistochemistry (IHC) (IHC) image
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 Immunofluorescence (IF) of anti-ACTN4 antibody    Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using alpha Actinin 4 antibody at 1: 200 dilution.
anti-ACTN4 antibody Immunofluorescence (IF) image
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NCBI/Uniprot data below describe general gene information for ACTN4. It may not necessarily be applicable to this product.
 NCBI GI #    13477151
 NCBI GeneID    81
 NCBI Accession #    AAH05033 [Other Products]
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 UniProt Secondary Accession #    O76048; A4K467; D6PXK4 [Other Products]
 UniProt Related Accession #    O43707 [Other Products]
 Molecular Weight    59,579 Da [Similar Products]
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 NCBI Official Full Name    Actinin, alpha 4
 NCBI Official Synonym Full Names    actinin, alpha 4
 NCBI Official Symbol    ACTN4 [Similar Products]
 NCBI Official Synonym Symbols   
FSGS; FSGS1; ACTININ-4
[Similar Products]
 NCBI Protein Information    alpha-actinin-4
 UniProt Protein Name    Alpha-actinin-4
 UniProt Synonym Protein Names   
Non-muscle alpha-actinin 4
 Protein Family    Alpha-actinin
 UniProt Gene Name    ACTN4 [Similar Products]
 UniProt Entry Name    ACTN4_HUMAN
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 NCBI Summary for ACTN4    Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq, Jul 2008]
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 UniProt Comments for ACTN4    ACTN4: F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Defects in ACTN4 are the cause of focal segmental glomerulosclerosis type 1 (FSGS1). A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation. Belongs to the alpha-actinin family.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 19q13

Cellular Component: cortical cytoskeleton; extracellular space; neuron projection; protein complex; focal adhesion; extracellular region; intercellular junction; pseudopodium; Z disc; ribonucleoprotein complex; perinuclear region of cytoplasm; cytoplasm; stress fiber; nucleus

Molecular Function: integrin binding; actin filament binding; protein binding; protein homodimerization activity; ligand-dependent nuclear receptor transcription coactivator activity; retinoic acid receptor binding; nucleoside binding; protein N-terminus binding; calcium ion binding; actin binding; nuclear hormone receptor binding

Biological Process: regulation of apoptosis; retinoic acid receptor signaling pathway; platelet activation; actin filament bundle formation; protein transport; platelet degranulation; response to hypoxia; negative regulation of cell motility; positive regulation of sodium:hydrogen antiporter activity; positive regulation of cell motility; blood coagulation; positive regulation of pinocytosis

Disease: Focal Segmental Glomerulosclerosis 1
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 Research Articles on ACTN4    1. Using fluorescence recovery after photobleaching, we show that an ACTN4 mutation that causes human kidney disease roughly triples the wild-type binding affinity of ACTN4 to F-actin in cells, increasing the dissociation time from 29 +/- 13 to 86 +/- 29 s.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-ACTN4 antibodyPathways associated with anti-ACTN4 antibody
 Reference Product  PubMed Publications
 ACTN1 antibody  >14 publications with ACTN4 and ACTN1
 SERPINE1 antibody  >2 publications with ACTN4 and SERPINE1
 PXN antibody  >2 publications with ACTN4 and PXN
 ACTN2 antibody  >2 publications with ACTN4 and ACTN2
 ITGB1 antibody  >1 publications with ACTN4 and ITGB1
 Products by Pathway  Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
 Cell-Cell Communication Pathway antibodies  Cell-Cell Communication Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
Diseases associated with anti-ACTN4 antibodyOrgans/Tissues associated with anti-ACTN4 antibody
 Disease Name  Pubmed Publications
 Kidney Diseases Antibodies  >97 publications with ACTN4 and Kidney Diseases
 Glomerulosclerosis, Focal Segmental Antibodies  >67 publications with ACTN4 and Glomerulosclerosis, Focal Segmental
 Proteinuria Antibodies  >39 publications with ACTN4 and Proteinuria
 Carcinoma Antibodies  >28 publications with ACTN4 and Carcinoma
 Neoplasm Metastasis Antibodies  >20 publications with ACTN4 and Neoplasm Metastasis
 Disease Models, Animal Antibodies  >16 publications with ACTN4 and Disease Models, Animal
 Adenocarcinoma Antibodies  >13 publications with ACTN4 and Adenocarcinoma
 Ovarian Diseases Antibodies  >7 publications with ACTN4 and Ovarian Diseases
 Hypertension Antibodies  >7 publications with ACTN4 and Hypertension
 Inflammation Antibodies  >6 publications with ACTN4 and Inflammation
 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >95 publications with ACTN4 and Kidney
 Blood Antibodies  >22 publications with ACTN4 and Blood
 Muscle Antibodies  >20 publications with ACTN4 and Muscle
 Lung Antibodies  >17 publications with ACTN4 and Lung
 Brain Antibodies  >10 publications with ACTN4 and Brain
 Vascular Antibodies  >10 publications with ACTN4 and Vascular
 Lymph Antibodies  >7 publications with ACTN4 and Lymph
 Lymph Node Antibodies  >7 publications with ACTN4 and Lymph Node
 Bone Antibodies  >6 publications with ACTN4 and Bone
 Heart Antibodies  >5 publications with ACTN4 and Heart
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